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1. HIV感染患者におけるCD4細胞数の測定手技および解釈 techniques and interpretation of measurement of the cd4 cell count in hiv infected patients
2. 世界的なヒト免疫不全ウイルスの大流行 the global human immunodeficiency virus pandemic
3. 自動血液分析装置 automated hematology instrumentation

English Journal

Direct grafting of poly(pentafluorophenyl acrylate) onto oxides: versatile substrates for reactive microcapillary printing and self-sorting modification.

Arnold RM1, McNitt CD, Popik VV, Locklin J.Author information 1Department of Chemistry, College of Engineering, and the Center for Nanoscale Science and Engineering, University of Georgia, Athens, GA 30602, USA. jlocklin@uga.edu.AbstractPoly(pentafluorophenyl acrylate) was covalently attached to silicon oxide through the direct coupling of the reactive ester to surface silanol groups. Subsequently, reactive microcapillary printing (R-μCaP) and a one-pot, self-sorting postpolymerization modification reaction were used to generate patterns of spatially resolved chemical functionality.
Chemical communications (Cambridge, England).Chem Commun (Camb).2014 May 25;50(40):5307-9. doi: 10.1039/c3cc47597b. Epub 2013 Nov 25.
Poly(pentafluorophenyl acrylate) was covalently attached to silicon oxide through the direct coupling of the reactive ester to surface silanol groups. Subsequently, reactive microcapillary printing (R-μCaP) and a one-pot, self-sorting postpolymerization modification reaction were used to generate p
PMID 24270591

Prevascularization of biofunctional calcium phosphate cement for dental and craniofacial repairs.

Chen W1, Thein-Han W2, Weir MD2, Chen Q3, Xu HH4.Author information 1State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China; Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA.2Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA.3State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China.4Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA; Center for Stem Cell Biology and Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA; University of Maryland Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD 21201, USA; Department of Mechanical Engineering, University of Maryland, Baltimore County, MD 21250, USA. Electronic address: hxu@umaryland.edu.AbstractOBJECTIVES: Calcium phosphate cement (CPC) is promising for dental and craniofacial repairs. Vascularization in bone tissue engineering constructs is currently a major challenge. The objectives of this study were to investigate the prevascularization of macroporous CPC via coculturing human umbilical vein endothelial cells (HUVEC) and human osteoblasts (HOB), and determine the effect of RGD in CPC on microcapillary formation for the first time.
Dental materials : official publication of the Academy of Dental Materials.Dent Mater.2014 May;30(5):535-44. doi: 10.1016/j.dental.2014.02.007.
OBJECTIVES: Calcium phosphate cement (CPC) is promising for dental and craniofacial repairs. Vascularization in bone tissue engineering constructs is currently a major challenge. The objectives of this study were to investigate the prevascularization of macroporous CPC via coculturing human umbilica
PMID 24731858

Localized Drug Application and Sub-Second Voltammetric Dopamine Release Measurements in a Brain Slice Perfusion Device.

Sun M1, Kaplan SV, Gehringer RC, Limbocker RA, Johnson MA.Author information 1Department of Chemistry and R. N. Adams Institute for Bioanalytical Chemistry, University of Kansas , Lawrence, Kansas 66045 United States.AbstractThe use of fast scan cyclic voltammetry (FSCV) to measure the release and uptake of dopamine (DA) as well as other biogenic molecules in viable brain tissue slices has gained popularity over the last 2 decades. Brain slices have the advantage of maintaining the functional three-dimensional architecture of the neuronal network while also allowing researchers to obtain multiple sets of measurements from a single animal. In this work, we describe a simple, easy-to-fabricate perfusion device designed to focally deliver pharmacological agents to brain slices. The device incorporates a microfluidic channel that runs under the perfusion bath and a microcapillary that supplies fluid from this channel up to the slice. We measured electrically evoked DA release in brain slices before and after the administration of two dopaminergic stimulants, cocaine and GBR-12909. Measurements were collected at two locations, one directly over and the other 500 μm away from the capillary opening. Using this approach, the controlled delivery of drugs to a confined region of the brain slice and the application of this chamber to FSCV measurements, were demonstrated. Moreover, the consumption of drugs was reduced to tens of microliters, which is thousands of times less than traditional perfusion methods. We expect that this simply fabricated device will be useful in providing spatially resolved delivery of drugs with minimum consumption for voltammetric and electrophysiological studies of a variety of biological tissues both in vitro and ex vivo.
Analytical chemistry.Anal Chem.2014 Apr 28. [Epub ahead of print]
The use of fast scan cyclic voltammetry (FSCV) to measure the release and uptake of dopamine (DA) as well as other biogenic molecules in viable brain tissue slices has gained popularity over the last 2 decades. Brain slices have the advantage of maintaining the functional three-dimensional architect
PMID 24734992

Japanese Journal

毛細血管の画像解析による血管状態の評価(研究発表,第38回生命情報科学シンポジウム)

永山勝也,三浦一郎,河越景史
Journal of International Society of Life Information Science 32(2), 199-204, 2014-09-01
指先血管の画像処理による評価法についての研究を行った.指先毛細血管の臨床画像を用いて,特徴点抽出により指先血管の輪郭の抽出を試みた.また,臨床画像より指先血管の状態について数値化を行い,自動的にパラメータを算出するプログラムを作成したことで処理の簡易化を実現した.さらに,画像処理により血管形状のパラメータを測定しその測定結果をサプリメントの性能評価に役立てることができた.
NAID 110009840856

Evaluation of desialylation effect on zeta potential of extracellular vesicles secreted from human prostate cancer cells by on-chip microcapillary electrophoresis

Akagi Takanori,Kato Kei,Hanamura Nami,Kobayashi Masashi,Ichiki Takanori
Jpn. J. Appl. Phys. 53(6S), 06JL01, 2014-05-12
NAID 150000109419

Nailfold microcapillary findings reveal early stage of congestion of right ventricle of the heart.

Miura Ichiro,Matsuo Masato,Konta Tsuyoshi,Nagayama Katsuya,Miyazaki Masami
Microvascular Reviews and Communications 7(1), 39a-39a, 2014
… Since the BNP rise and this finding had a significant difference statistically, we considered nailfold microcapillary findings can reveal early stage of congestion of right ventricle of the heart. …
NAID 130004678495