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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2012/11/23 22:50:34」(JST)

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A metalloproteinase, or metalloprotease, is any protease enzyme whose catalytic mechanism involves a metal.

Most metalloproteases require zinc, but some use cobalt. The metal ion is coordinated to the protein via three ligands. The ligands co-ordinating the metal ion can vary with histidine, glutamate, aspartate, lysine, and arginine.^{[clarification needed]} The fourth coordination position is taken up by a labile water molecule.

Treatment with chelating agents such as EDTA leads to complete inactivation. EDTA is a metal chelator that removes zinc, which is essential for activity. They are also inhibited by the chelator orthophenanthroline.

Classification

There are two subgroups of metalloproteinases:

Exopeptidases, metalloexopeptidases (EC number: 3.4.17).
Endopeptidases, metalloendopeptidases (3.4.24). Well known metalloendopeptidases include ADAM proteins and matrix metalloproteinases.

In the MEROPS database peptidase families are grouped by their catalytic type, the first character representing the catalytic type: A, aspartic; C, cysteine; G, glutamic acid; M, metallo; S, serine; T, threonine; and U, unknown. The serine, threonine and cysteine peptidases utilise the amino acid as a nucleophile and form an acyl intermediate - these peptidases can also readily act as transferases. In the case of aspartic, glutamic and metallopeptidases, the nucleophile is an activated water molecule. In many instances the structural protein fold that characterises the clan or family may have lost its catalytic activity, yet retain its function in protein recognition and binding.

Metalloproteases are the most diverse of the four main protease types, with more than 50 families classified to date. In these enzymes, a divalent cation, usually zinc, activates the water molecule. The metal ion is held in place by amino acid ligands, usually three in number. The known metal ligands are His, Glu, Asp or Lys and at least one other residue is required for catalysis, which may play an electrophillic role. Of the known metalloproteases, around half contain an HEXXH motif, which has been shown in crystallographic studies to form part of the metal-binding site.^[1] The HEXXH motif is relatively common, but can be more stringently defined for metalloproteases as 'abXHEbbHbc', where 'a' is most often valine or threonine and forms part of the S1' subsite in thermolysin and neprilysin, 'b' is an uncharged residue, and 'c' a hydrophobic residue^[2] . Proline is never found in this site, possibly because it would break the helical structure adopted by this motif in metalloproteases.^[1]

Metallopeptidases from family M48 are integral membrane proteins associated with the endoplasmic reticulum and Golgi, binding one zinc ion per subunit. These endopeptidases include CAAX prenyl protease 1, which proteolytically removes the C-terminal three residues of farnesylated proteins.

Members of the M50 metallopeptidase family include: mammalian sterol-regulatory element binding protein (SREBP) site 2 protease and Escherichia coli protease EcfE, stage IV sporulation protein FB.

References

^ ^a ^b Rawlings ND, Barrett AJ (1995). "Evolutionary families of metallopeptidases". Meth. Enzymol. 248: 183-228. doi:10.1016/0076-6879(95)48015-3. PMID 7674922.
^ . http://www.plosone.org/article/fetchObjectAttachment.action;jsessionid=CE17B6912F77B4069E4969431710B8A7?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0046147&representation=PDF.

External links

The MEROPS online database for peptidases and their inhibitors: Metallo Peptidases
Metalloproteases at the US National Library of Medicine Medical Subject Headings (MeSH)
Metalloproteinase at eMedicine Dictionary
Proteopedia: Metalloproteases

This hydrolase article is a stub. You can help Wikipedia by expanding it.

This article incorporates text from the public domain Pfam and InterPro IPR008915

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Peptidase_M50
Identifiers
Symbol	Peptidase_M50
Pfam	PF02163
Pfam clan	CL0126
InterPro	IPR008915
MEROPS	M50
OPM superfamily	205
OPM protein	3b4r
Available protein structures:
Pfam	structures
PDB	RCSB PDB; PDBe
PDBsum	structure summary

Peptidase_M48
Identifiers
Symbol	Peptidase_M48
Pfam	PF01435
Pfam clan	CL0126
InterPro	IPR001915
MEROPS	M48
OPM superfamily	460
OPM protein	4aw6
Available protein structures:
Pfam	structures
PDB	RCSB PDB; PDBe
PDBsum	structure summary

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[pmid7674922-1] Rawlings ND, Barrett AJ (1995). "Evolutionary families of metallopeptidases". Meth. Enzymol. 248: 183-228. doi:10.1016/0076-6879(95)48015-3. PMID 7674922.

[2] . http://www.plosone.org/article/fetchObjectAttachment.action;jsessionid=CE17B6912F77B4069E4969431710B8A7?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0046147&representation=PDF.

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metalloproteinases