WordNet

measuring instrument consisting of a graduated glass tube used to measure or transfer precise volumes of a liquid by drawing the liquid up into the tube (同)pipette

PrepTutorEJDIC

全文を閲覧するには購読必要です。 To read the full text you will need to subscribe.

Biophysical implications of lipid bilayer rheometry for mechanosensitive channels.

Bavi N1, Nakayama Y2, Bavi O3, Cox CD2, Qin QH4, Martinac B5.
Proceedings of the National Academy of Sciences of the United States of America.Proc Natl Acad Sci U S A.2014 Sep 23;111(38):13864-9. doi: 10.1073/pnas.1409011111. Epub 2014 Sep 8.
The lipid bilayer plays a crucial role in gating of mechanosensitive (MS) channels. Hence it is imperative to elucidate the rheological properties of lipid membranes. Herein we introduce a framework to characterize the mechanical properties of lipid bilayers by combining micropipette aspiration (MA)
PMID 25201991

Make it spin: individual trapping of sperm for analysis and recovery using micro-contact printing.

Frimat JP1, Bronkhorst M, de Wagenaar B, Bomer JG, van der Heijden F, van den Berg A, Segerink LI.
Lab on a chip.Lab Chip.2014 Aug 7;14(15):2635-41. doi: 10.1039/c4lc00050a. Epub 2014 Mar 11.
In this article, we describe the development of a high throughput platform to spatially manipulate viable sperm for motility measurements and recovery of the best single sperm for fertilization purposes. Micro-contact printing was used to pattern islands of adhesive proteins (fibronectin) separated
PMID 24615285

Alansary D, Kilch T, Holzmann C, Peinelt C, Hoth M, Lis A.
Cold Spring Harbor protocols.Cold Spring Harb Protoc.2014 Jun 2;2014(6):630-7. doi: 10.1101/pdb.prot073254.
Although ICRAC and other store-operated currents are often analyzed by Ca(2+) imaging, whole-cell patch clamp, described here, is the preferred technique to analyze ICRAC whenever possible. The whole-cell patch-clamp protocol can even be used to record endogenous ICRAC in primary cells. The small en
PMID 24890203

大野豪,佐々木智基,中村隆文 [他],原口大,浦崎貴美子,小濱継雄
日本応用動物昆虫学会誌 50(1), 51-56, 2006-02-25
イモゾウムシの卵数の推定法は,本種の人工飼育における,採卵効率の評価と飼料への卵接種密度の制御のために不可欠である.そこで,本種の卵数推定法を,卵体積に対する卵数の回帰分析によって作った.まず,2種類のメスピペット(1mlと2ml)を用いて卵を複数回異なる体積で量りとり,含まれる卵数を数えた.共分散分析により,卵体積の増加に対する卵数の増加パターンがピペットの種類間で異なることが示されたため,ピペ …
NAID 110004659451

, , , , ,
THE TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE 191(1), 1-5, 2000-05-01
… In all of the described techniques, epididymal material was drawn into a micro-pipette before counting procedure. … We aimed to proceed this stage without using any pipette because of the difficulty in aspirating very viscous epididymal material into a micro-pipette. … For measuring epididymal material we used a 2 μl reservoir which we named as a micro-spoon. …
NAID 10013014929