リ・フェクション、リ・フェクション法

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/06/26 21:43:55」(JST)

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Lipofection (or liposome transfection) is a technique used to inject genetic material into a cell by means of liposomes, which are vesicles that can easily merge with the cell membrane since they are both made of a phospholipid bilayer. Lipofection generally uses a positively charged (cationic) lipid to form an aggregate with the negatively charged (anionic) genetic material.^[1] A net positive charge on this aggregrate has been assumed to increase the effectiveness of transfection through the negatively charged phospholipid bilayer.^[1] This transfection technology performs the same tasks as other biochemical procedures utilizing polymers, DEAE dextran, calcium phosphate, and electroporation. The main advantages of lipofection are its high efficiency, its ability to transfect all types of nucleic acids in a wide range of cell types, its ease of use, reproducibility, and low toxicity. In addition, this method is suitable for all transfection applications (transient, stable, co-transfection, reverse, sequential or multiple transfections). High throughput screening assay has also shown good efficiency in some in vivo models.

References

Felgner PL, Gadek TR, Holm M et al. (November 1987). "Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure". Proc. Natl. Acad. Sci. U.S.A. 84 (21): 7413–7. doi:10.1073/pnas.84.21.7413. PMC 299306. PMID 2823261.
Felgner JH, Kumar R, Sridhar CN et al. (January 1994). "Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations". J. Biol. Chem. 269 (4): 2550–61. PMID 8300583.

Notes

^ ^a ^b http://www.freepatentsonline.com/EP1129064.pdf

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English Journal

MIC3, a novel cross-protective antigen expressed in Toxoplasma gondii and Neospora caninum.

Yang D1, Liu J, Hao P, Wang J, Lei T, Shan D, Liu Q.
Parasitology research.Parasitol Res.2015 Oct;114(10):3791-9. doi: 10.1007/s00436-015-4609-6. Epub 2015 Jul 5.
Microneme protein 3 (MIC3) is an important adhesion molecule expressed by Toxoplasma gondii and Neospora caninum that plays a crucial role in invasion. In our study, we found that recombinant TgMIC3 (rTgMIC3) was recognized by both T. gondii-reactive sera and hyper-immune serum against N. caninum. P
PMID 26141436

Characterization of exogenous DNA mobility in live cells through fluctuation correlation spectroscopy.

Mieruszynski S1, Digman MA2,3,4, Gratton E2,3,4, Jones MR1.
Scientific reports.Sci Rep.2015 Sep 10;5:13848. doi: 10.1038/srep13848.
The spatial-temporal dynamics of delivered DNA is a critical aspect influencing successful gene delivery. A comprehensive model of DNA lipoplex trafficking through live cells has yet to be demonstrated. Here the bioimaging approaches Raster Image Correlation Spectroscopy (RICS) and image-Means Squar
PMID 26354725

Electroporation Knows No Boundaries: The Use of Electrostimulation for siRNA Delivery in Cells and Tissues.

Luft C1, Ketteler R2.
Journal of biomolecular screening.J Biomol Screen.2015 Sep;20(8):932-42. doi: 10.1177/1087057115579638. Epub 2015 Apr 7.
The discovery of RNA interference (RNAi) has enabled several breakthrough discoveries in the area of functional genomics. The RNAi technology has emerged as one of the major tools for drug target identification and has been steadily improved to allow gene manipulation in cell lines, tissues, and who
PMID 25851034

Japanese Journal

CREB-binding protein transcription activation domain for enhanced transgene expression by a positive feedback system

Kanda Genki,Ochiai Hiroshi,Harashima Hideyoshi,Kamiya Hiroyuki
Journal of Biotechnology 157(1), 7-11, 2012-01
… Plasmid DNAs encoding the transcription factors were introduced into mouse Hepa 1-6 cells by electroporation and lipofection. …
NAID 120003811532

オステオポンチンの多機能性と臨床応用への戦略 (第59回日本泌尿器科学会中部総会(金沢) シンポジウム尿路結石の概念を変える,治療・予防法を変える)

辻秀憲,梅川徹,植村天受
泌尿器科紀要 57(1), 49-54, 2011-01
… Hydrodynamic intravenous and renal subcapsular injections with lipofection were performed on days 1 and 8. …
NAID 120002770957