WordNet

convert into a sulfate
a salt or ester of sulphuric acid (同)sulphate

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2014/09/12 17:00:19」(JST)

wiki en

Keratan sulfate (KS), also called keratosulfate, is any of several sulfated glycosaminoglycans (structural carbohydrates) that have been found especially in the cornea, cartilage, and bone. It is also synthesized in the central nervous system where it participates both in development^[1] and in the glial scar formation following an injury.^[2] Keratan sulfates are large, highly hydrated molecules which in joints can act as a cushion to absorb mechanical shock.

Keratan sulfate structure

Like other glycosaminoglycans keratan sulfate is a linear polymer that consists of a repeating disaccharide unit. Keratan sulfate occurs as a proteoglycan (PG) in which KS chains are attached to cell-surface or extracellular matrix proteins, termed core proteins. KS core proteins include Lumican, Keratocan, Mimecan, Fibromodulin, PRELP, Osteoadherin and Aggrecan.

The basic repeating disaccharide unit within keratan sulfate is -3Galβ1-4GlcNAcβ1-. This can be sulfated at carbon position 6 (C6) of either or both the Gal or GlcNAc monosaccharides. However, the detailed primary structure of specific KS types are best considered to be composed of three regions:^[3]

A linkage region, at one end of which the KS chain is linked to the core protein.
A repeat region, composed of the -3Galβ1-4GlcNAcβ1- repeating disaccharide unit and
A chain capping region, occurring at the opposite end of the KS chain to the protein linkage region.

The monosaccharide mannose is found within the linkage region of keratan sulfate type I (KSI). Disaccharides within the repeating region of KSII may be fucosylated and N-Acetylneuraminic acid caps the end of all keratan sulfate type II (KSII) chains and up to 70% of KSI type chains.^[4]

KS classes

The designations KSI and KSII were originally assigned on the basis of the tissue type from which the keratan sulfate was isolated. KSI was isolated from corneal tissue and KSII from skeletal tissue.^[5]^[6] Minor monosaccharide compositional differences exist between KS extracted from both sources and even KS extracted from the same source. However, major differences occur in the way each KS type is joined to its core protein.^[7] The designations KSI and KSII are now based upon these protein linkage differences. KSI is N-linked to specific asparagine amino acids via N-acetylglucosamine and KSII is O-linked to specific Serine or Threonine amino acids via N-acetyl galactosamine.^[8] The tissue based classification of KS no longer exists as KS types have been shown to be non tissue specific.^[4] A third type of KS (KSIII) has also been isolated from brain tissue that is O-linked to specific serine or threonine amino acids via mannose.^[9]

Corneal KSI

The amount of KS found in the cornea is 10 fold higher than it is in cartilage and 2-4 times higher than it is in other tissues.^[10] It is produced by corneal keratocytes ^[11] and is thought to play a role of a dynamic buffer of corneal hydration. In a rare progressive disorder called macular corneal dystrophy (MCDC), the synthesis of keratan sulfate is either absent (MCDC type I) or abnormal (MCDC type II).^[12]

Non-corneal KSI

Osteoadherin, Fibromodulin and PRELP are core proteins found in bone and cartilage, that are modified by N-linked KS chains.

KSII

KSIII

References

^ Miller B, Sheppard AM, Pearlman AL (April 1997). "Developmental expression of keratan sulfate-like immunoreactivity distinguishes thalamic nuclei and cortical domains". J. Comp. Neurol. 380 (4): 533–52. doi:10.1002/(SICI)1096-9861(19970421)380:4<533::AID-CNE9>3.0.CO;2-2. PMID 9087531.
^ Zhang H, Uchimura K, Kadomatsu K (November 2006). "Brain keratan sulfate and glial scar formation". Ann. N. Y. Acad. Sci. 1086: 81–90. Bibcode:2006NYASA1086...81Z. doi:10.1196/annals.1377.014. PMID 17185507.
^ Tai GH, Huckerby TN and Nieduszynski IA. (1996). "Multiple non-reducing chain termini isolated from bovine corneal keratan sulfates". J. Biol. Chem. 271 (38): 23535–23546. doi:10.1074/jbc.271.38.23535. PMID 8798563.
^ ^a ^b Funderburgh JL. (2000). "Keratan sulfate: structure, biosynthesis, and function". Glycobiology 10 (10): 951–958. doi:10.1093/glycob/10.10.951. PMID 11030741.
^ Meyer K, Linker A. et al. (1 December 1953). "The mucopolysaccharides of bovine cornea". J. Biol. Chem. 205 (2): 611–616. PMID 13129238.
^ Meyer K, Hoffman P. and Linker A. (1958). "Mucopolysaccharides of Costal Cartilage". Science 128 (3329): 896. Bibcode:1958Sci...128..896M. doi:10.1126/science.128.3329.896. PMID 13592269.
^ Seno N, Meyer K. et al. (1 March 1965). "Variations in Keratosulfates". J. Biol. Chem. 240 (3): 1005–1019. PMID 14284693.
^ Nieduszynski IA, Huckerby TN. et al. (1990). "There are two major types of skeletal keratan sulphates". Biochem. J. 271 (1): 243–245. PMC 1149539. PMID 2222415.
^ Krusius T, Finne J. et al. (25 June 1986). "Identification of an O-glycosidic mannose-linked sialylated tetrasaccharide and keratan sulfate oligosaccharides in the chondroitin sulfate proteoglycan of brain". J. Biol. Chem. 261 (18): 8237–8242. PMID 2941416.
^ Funderburgh JL, Caterson B. and Conrad GW. (1987). "Distribution of proteoglycans antigenically related to corneal keratan sulfate proteoglycan". J. Biol. Chem. 262 (24): 11634–11640. PMID 2957372.
^ Funderburgh JL, Mann MM, Funderburgh ML (November 2003). "Keratocyte phenotype mediates proteoglycan structure: a role for fibroblasts in corneal fibrosis". J. Biol. Chem. 278 (46): 45629–37. doi:10.1074/jbc.M303292200. PMC 2877919. PMID 12933807.
^ Macular dystrophy, corneal, 1 - OMIM

External links

Chondroitin_and_keratin_sulfate at the Duke University Health System's Orthopedics program
Keratan sulfate at the US National Library of Medicine Medical Subject Headings (MeSH)

UpToDate Contents

全文を閲覧するには購読必要です。 To read the full text you will need to subscribe.

1. ムコ多糖症：臨床的特徴および診断 mucopolysaccharidoses clinical features and diagnosis
2. 硫酸マグネシウムへの子宮内曝露の神経保護効果 neuroprotective effects of in utero exposure to magnesium sulfate
3. 成人におけるS状結腸鏡検査および大腸内視鏡検査のための前処置 bowel preparation for colonoscopy and flexible sigmoidoscopy in adults
4. 関節リウマチの病因 pathogenesis of rheumatoid arthritis
5. デヒドロエピアンドロステロンおよびその硫酸塩 dehydroepiandrosterone and its sulfate

English Journal

Dual immunofluorescence staining of proteoglycans in human temporal bones.

Markaryan A, Nelson EG, Kohut RI, Hinojosa R.SourceSection of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of Chicago, Chicago, Illinois, U.S.A.; House Ear Institute, Los Angeles, California, U.S.A.
The Laryngoscope.Laryngoscope.2011 Jul;121(7):1525-31. doi: 10.1002/lary.21772. Epub 2011 Jun 6.
OBJECTIVES/HYPOTHESIS: Immunofluorescence staining methods have been developed to study the distribution of macromolecules in archival formalin-fixed celloidin-embedded human temporal bone tissues. The aim of this study was to investigate the feasibility of utilizing this approach to evaluate the co
PMID 21647895

Japanese Journal

Evaluation of a new enzyme-linked immunosorbent assay to detect keratan sulfate in equine serum

Lettry Vivien,Kawasaki Hirofumi,Sugaya Kiyofumi,Hosoya Kenji,Takagi Satoshi,Okumura Masahiro
Japanese Journal of Veterinary Research 57(4), 207-212, 2010-02
… NoteThis study aimed to evaluate a system that identifies cartilage turn over and/or degradation through measurement of a new keratan sulfate (KS) epitope concentration in equine sera. …
NAID 120001944336