ピロリン酸 PPi

WordNet

relating or belonging to the class of compounds not having a carbon basis; "hydrochloric and sulfuric acids are called inorganic substances"
lacking the properties characteristic of living organisms
a salt or ester of pyrophosphoric acid

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無生物の / 無機の

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1. ピロリン酸カルシウム結晶沈着症の臨床症状および診断 clinical manifestations and diagnosis of calcium pyrophosphate crystal deposition disease
2. ピロリン酸カルシウム結晶沈着症の治療 treatment of calcium pyrophosphate crystal deposition disease
3. 変形性関節症の病因 pathogenesis of osteoarthritis
4. ピロリン酸カルシウム結晶沈着症の発症および病因 pathogenesis and etiology of calcium pyrophosphate crystal deposition disease
5. 骨軟化症の臨床症状、診断、および治療 clinical manifestations diagnosis and treatment of osteomalacia

English Journal

Metabolic flux analysis of recombinant Pichia pastoris growing on different glycerol/methanol mixtures by iterative fitting of NMR-derived (13)C-labelling data from proteinogenic amino acids.

Jordà J, de Jesus SS, Peltier S, Ferrer P, Albiol J.Author information Departament d'Enginyeria Química, Escola d'Enginyeria, Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Spain.AbstractThe yeast Pichia pastoris has emerged as one of the most promising yeast cell factories for the production of heterologous proteins. The readily available genetic tools and the ease of high-cell density cultivations using methanol or glycerol/methanol mixtures are among the key factors for this development. Previous studies have shown that the use of mixed feeds of glycerol and methanol seem to alleviate the metabolic burden derived from protein production, allowing for higher specific and volumetric process productivities. However, initial studies of glycerol/methanol co-metabolism in P. pastoris by classical metabolic flux analyses using (13)C-derived Metabolic Flux Ratio (METAFoR) constraints were hampered by the reduced labelling information obtained when using C3:C1 substrate mixtures in relation to the conventional C6 substrate, that is, glucose. In this study, carbon flux distributions through the central metabolic pathways in glycerol/methanol co-assimilation conditions have been further characterised using biosynthetically directed fractional (13)C labelling. In particular, metabolic flux distributions were obtained under 3 different glycerol/methanol ratios and growth rates by iterative fitting of NMR-derived (13)C-labelling data from proteinogenic amino acids using the software tool (13)CFlux2. Specifically, cells were grown aerobically in chemostat cultures fed with 80:20, 60:40 and 40:60 (w:w) glycerol/methanol mixtures at two dilutions rates (0.05hour(-1) and 0.16hour(-1)), allowing to obtain additional data (biomass composition and extracellular fluxes) to complement pre-existing datasets. The performed (13)C-MFA reveals a significant redistribution of carbon fluxes in the central carbon metabolism as a result of the shift in the dilution rate, while the ratio of carbon sources has a lower impact on carbon flux distribution in cells growing at the same dilution rate. At low growth rate, the percentage of methanol directly dissimilated to CO2 ranges between 50% and 70%. At high growth rate the methanol is completely dissimilated to CO2 by the direct pathway, in the two conditions of highest methanol content.
New biotechnology.N Biotechnol.2014 Jan 25;31(1):120-32. doi: 10.1016/j.nbt.2013.06.007. Epub 2013 Jul 8.
The yeast Pichia pastoris has emerged as one of the most promising yeast cell factories for the production of heterologous proteins. The readily available genetic tools and the ease of high-cell density cultivations using methanol or glycerol/methanol mixtures are among the key factors for this deve
PMID 23845285

Transcriptome analysis of Capsicum annuum varieties Mandarin and Blackcluster: Assembly, annotation and molecular marker discovery.

Ahn YK, Tripathi S, Kim JH, Cho YI, Lee HE, Kim DS, Woo JG, Cho MC.Author information Vegetable Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Suwon 440-706, Republic of Korea. Electronic address: aykyun@korea.kr.AbstractNext generation sequencing technologies have proven to be a rapid and cost-effective means to assemble and characterize gene content and identify molecular markers in various organisms. Pepper (Capsicum annuum L., Solanaceae) is a major staple vegetable crop, which is economically important and has worldwide distribution. High-throughput transcriptome profiling of two pepper cultivars, Mandarin and Blackcluster, using 454 GS-FLX pyrosequencing yielded 279,221 and 316,357 sequenced reads with a total 120.44 and 142.54Mb of sequence data (average read length of 431 and 450 nucleotides). These reads resulted from 17,525 and 16,341 'isogroups' and were assembled into 19,388 and 18,057 isotigs, and 22,217 and 13,153 singletons for both the cultivars, respectively. Assembled sequences were annotated functionally based on homology to genes in multiple public databases. Detailed sequence variant analysis identified a total of 9701 and 12,741 potential SNPs which eventually resulted in 1025 and 1059 genotype specific SNPs, for both the varieties, respectively, after examining SNP frequency distribution for each mapped unigenes. These markers for pepper will be highly valuable for marker-assisted breeding and other genetic studies.
Gene.Gene.2014 Jan 10;533(2):494-9. doi: 10.1016/j.gene.2013.09.095. Epub 2013 Oct 11.
Next generation sequencing technologies have proven to be a rapid and cost-effective means to assemble and characterize gene content and identify molecular markers in various organisms. Pepper (Capsicum annuum L., Solanaceae) is a major staple vegetable crop, which is economically important and has
PMID 24125952

ABCC6 prevents ectopic mineralization seen in pseudoxanthoma elasticum by inducing cellular nucleotide release.

Jansen RS, Küçükosmanoglu A, de Haas M, Sapthu S, Otero JA, Hegman IE, Bergen AA, Gorgels TG, Borst P, van de Wetering K.Author information Divisions of Molecular Oncology and Gene Regulation, Netherlands Cancer Institute, 1066 CX, Amsterdam, The Netherlands.AbstractPseudoxanthoma elasticum (PXE) is an autosomal recessive disease characterized by progressive ectopic mineralization of the skin, eyes, and arteries, for which no effective treatment exists. PXE is caused by inactivating mutations in the gene encoding ATP-binding cassette sub-family C member 6 (ABCC6), an ATP-dependent efflux transporter present mainly in the liver. Abcc6(-/-) mice have been instrumental in demonstrating that PXE is a metabolic disease caused by the absence of an unknown factor in the circulation, the presence of which depends on ABCC6 in the liver. Why absence of this factor results in PXE has remained a mystery. Here we report that medium from HEK293 cells overexpressing either human or rat ABCC6 potently inhibits mineralization in vitro, whereas medium from HEK293 control cells does not. Untargeted metabolomics revealed that cells expressing ABCC6 excrete large amounts of nucleoside triphosphates, even though ABCC6 itself does not transport nucleoside triphosphates. Extracellularly, ectonucleotidases hydrolyze the excreted nucleoside triphosphates to nucleoside monophosphates and inorganic pyrophosphate (PPi), a strong inhibitor of mineralization that plays a pivotal role in several mineralization disorders similar to PXE. The in vivo relevance of our data are demonstrated in Abcc6(-/-) mice, which had plasma PPi levels <40% of those found in WT mice. This study provides insight into how ABCC6 affects PXE. Our data indicate that the factor that normally prevents PXE is PPi, which is provided to the circulation in the form of nucleoside triphosphates via an as-yet unidentified but ABCC6-dependent mechanism.
Proceedings of the National Academy of Sciences of the United States of America.Proc Natl Acad Sci U S A.2013 Dec 10;110(50):20206-11. doi: 10.1073/pnas.1319582110. Epub 2013 Nov 25.
Pseudoxanthoma elasticum (PXE) is an autosomal recessive disease characterized by progressive ectopic mineralization of the skin, eyes, and arteries, for which no effective treatment exists. PXE is caused by inactivating mutations in the gene encoding ATP-binding cassette sub-family C member 6 (ABCC
PMID 24277820

Japanese Journal

Roles of the vacuolar H+-PPase in seed storage oil mobilization and plant development

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PLANT MORPHOLOGY 26(1), 45-51, 2014
… In the case of Arabidopsis thaliana, an oilseed species, we characterized the importance of cytosolic inorganic pyrophosphate hydrolysis for the success of the above transition and appropriate execution of postembryonic developmental programs. …
NAID 130005066549

PHOSPHATE GLASSES AND GLASS-CERAMICS FOR BIOMEDICAL APPLICATIONS

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Phosphorus Research Bulletin 26(0), 8-15, 2012
… The glasses include orthophosphate and pyrophosphate groups. …
NAID 130004502151

Label-free Fluorescent Detection of Loop-mediated Isothermal Amplification of Nucleic Acid Using Pyrophosphate-selective Xanthene-based Zn(II)-coordination Chemosensor

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Chemistry Letters 41(12), 1666-1668, 2012
… A label-free fluorescent detection method for the loop-mediated isothermal amplification (LAMP) of nucleic acids was first developed by using the pyrophosphate-selective xanthene-based Zn(II)-coordination chemosensor 1–2Zn(II). … In this method, the production of inorganic pyrophosphate (PPi), a by-product of the LAMP reaction, was detected by 1–2Zn(II) with a large fluorescence enhancement. …
NAID 130004426481

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