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1. 拡張型心筋症の遺伝学 genetics of dilated cardiomyopathy
2. GISTを含む消化管間葉系腫瘍の疫学、分類、臨床像、予後の特徴、および診断方法 epidemiology classification clinical presentation prognostic features and diagnostic work up of gastrointestinal mesenchymal neoplasms including gist

English Journal

Glial fibrillary acidic protein isoform expression in plaque related astrogliosis in Alzheimer's disease.

Kamphuis W, Middeldorp J, Kooijman L, Sluijs JA, Kooi EJ, Moeton M, Freriks M, Mizee MR, Hol EM.Author information Netherlands Institute for Neuroscience-an Institute of the Royal Netherlands Academy of Arts and Sciences (KNAW), Department of Astrocyte Bology and Neurodegeneration, Amsterdam, the Netherlands. Electronic address: w.kamphuis@nin.knaw.nl.AbstractIn Alzheimer's disease (AD), amyloid plaques are surrounded by reactive astrocytes with an increased expression of intermediate filaments including glial fibrillary acidic protein (GFAP). Different GFAP isoforms have been identified that are differentially expressed by specific subpopulations of astrocytes and that impose different properties to the intermediate filament network. We studied transcript levels and protein expression patterns of all known GFAP isoforms in human hippocampal AD tissue at different stages of the disease. Ten different transcripts for GFAP isoforms were detected at different abundancies. Transcript levels of most isoforms increased with AD progression. GFAPδ-immunopositive astrocytes were observed in subgranular zone, hilus, and stratum-lacunosum-moleculare. GFAPδ-positive cells also stained for GFAPα. In AD donors, astrocytes near plaques displayed increased staining of both GFAPα and GFAPδ. The reading-frame-shifted isoform, GFAP(+1), staining was confined to a subset of astrocytes with long processes, and their number increased in the course of AD. In conclusion, the various GFAP isoforms show differential transcript levels and are upregulated in a concerted manner in AD. The GFAP(+1) isoform defines a unique subset of astrocytes, with numbers increasing with AD progression. These data indicate the need for future exploration of underlying mechanisms concerning the functions of GFAPδ and GFAP(+1) isoforms in astrocytes and their possible role in AD pathology.
Neurobiology of aging.Neurobiol Aging.2014 Mar;35(3):492-510. doi: 10.1016/j.neurobiolaging.2013.09.035. Epub 2013 Oct 23.
In Alzheimer's disease (AD), amyloid plaques are surrounded by reactive astrocytes with an increased expression of intermediate filaments including glial fibrillary acidic protein (GFAP). Different GFAP isoforms have been identified that are differentially expressed by specific subpopulations of ast
PMID 24269023

Characterization of secretagogin-immunoreactive amacrine cells in marmoset retina.

Weltzien F, Dimarco S, Protti DA, Daraio T, Martin PR, Grünert U.Author information Department of Ophthalmology and Save Sight Institute, University of Sydney, Australia; Australian Research Council Centre of Excellence in Vision Science, University of Sydney, Australia.AbstractThe retina contains at least 30 different types of amacrine cells but not many are well characterized. In the present study the calcium-binding protein secretagogin was localized in a population of regular and displaced amacrine cells in the retina of the common marmoset Callithrix jacchus. Irrespective of their soma location, the dendrites of secretagogin amacrine cells occupy strata 2, 3, and 4 of the inner plexiform layer, between the two bands formed by cholinergic amacrine cells. Segretagogin amacrine cells are also immunopositive to antibodies against glutamic acid decarboxylase, suggesting that they use γ-aminobutyric acid (GABA) as their neurotransmitter. The spatial density of secretagogin amacrine cells decreases from a peak of about 400 cells/mm(2) near 1 mm eccentricity to less than 100 cells/mm(2) in peripheral retina; these densities account for about 1% of amacrine cells in the inner nuclear layer and for up to 27% of displaced amacrine cells. The cell bodies form a regular mosaic, suggesting that they constitute a single amacrine cell population. Secretagogin cells have varicose dendrites, which are decorated with small spines. Intracellular injection of DiI into secretagogin cells revealed an average dendritic field diameter of 170 μm and an average coverage factor of 3.2. In summary, secretagogin cells in marmoset retina are medium-field amacrine cells that share their stratification pattern with narrow-field amacrine cells and their neurotransmitter with wide-field amacrine cells. They may mediate spatial inhibition spanning the centralmost (on and off) bands of the inner plexiform layer. J. Comp. Neurol. 522:435-455, 2014. © 2013 Wiley Periodicals, Inc.
The Journal of comparative neurology.J Comp Neurol.2014 Feb;522(2):435-55. doi: 10.1002/cne.23420.
The retina contains at least 30 different types of amacrine cells but not many are well characterized. In the present study the calcium-binding protein secretagogin was localized in a population of regular and displaced amacrine cells in the retina of the common marmoset Callithrix jacchus. Irrespec
PMID 23852983

Serotonin-immunoreactive sensory neurons in the antenna of the cockroach Periplaneta americana.

Watanabe H, Shimohigashi M, Yokohari F.Author information Division of Biology, Department of Earth System Science, Fukuoka University, Fukuoka, Japan.AbstractThe antennae of insects contain a vast array of sensory neurons that process olfactory, gustatory, mechanosensory, hygrosensory, and thermosensory information. Except those with multimodal functions, most sensory neurons use acetylcholine as a neurotransmitter. Using immunohistochemistry combined with retrograde staining of antennal sensory neurons in the cockroach Periplaneta americana, we found serotonin-immunoreactive sensory neurons in the antenna. These were selectively distributed in chaetic and scolopidial sensilla and in the scape, the pedicel, and first 15 segments of the flagellum. In a chaetic sensillum, A single serotonin-immunoreactive sensory neuron cohabited with up to four serotonin-negative sensory neurons. Based on their morphological features, serotonin-immunopositive and -negative sensory neurons might process mechanosensory and contact chemosensory modalities, respectively. Scolopidial sensilla constitute the chordotonal and Johnston's organs within the pedicel and process antennal vibrations. Immunoelectron microscopy clearly revealed that serotonin-immunoreactivities selectively localize to a specific type of mechanosensory neuron, called type 1 sensory neuron. In a chordotonal scolopidial sensillum, a serotonin-immunoreactive type 1 neuron always paired with a serotonin-negative type 1 neuron. Conversely, serotonin-immunopositive and -negative type 1 neurons were randomly distributed in Johnston's organ. In the deutocerebrum, serotonin-immunoreactive sensory neuron axons formed three different sensory tracts and those from distinct types of sensilla terminated in distinct brain regions. Our findings indicate that a biogenic amine, serotonin, may act as a neurotransmitter in peripheral mechanosensory neurons. J. Comp. Neurol. 522:414-434, 2014. © 2013 Wiley Periodicals, Inc.
The Journal of comparative neurology.J Comp Neurol.2014 Feb;522(2):414-34. doi: 10.1002/cne.23419.
The antennae of insects contain a vast array of sensory neurons that process olfactory, gustatory, mechanosensory, hygrosensory, and thermosensory information. Except those with multimodal functions, most sensory neurons use acetylcholine as a neurotransmitter. Using immunohistochemistry combined wi
PMID 23852943

Japanese Journal

ラット島皮質におけるparvalbumin陽性細胞の分布特性

別府祐次
明海歯科医学 43(1), 52-62, 2014
NAID 40019994861

Immunohistochemical Detection of Aurora A and ERK Pathway in Oral Leukoplakia and Oral Squamous Cell Carcinoma

Dong Yan,Li Zhiyong,Gao Mingyang,Liu Caiyun,Liu Han,Chen Zhuwen,Xiao Jing
Journal of Hard Tissue Biology 23(1), 71-76, 2014
… In addition, more than 50% pERK1/2 immunopositive cells appears to be a good cut off value to predict the malignant transformation of OLK. …
NAID 130004480640

Pathophysiology and treatment of subclinical Cushing's disease and pituitary silent corticotroph adenomas [Review]

Kageyama Kazunori,Oki Yutaka,Nigawara Takeshi,Suda Toshihiro,Daimon Makoto
Endocrine Journal, 2014
… Silent corticotroph adenomas (SCAs) are defined by normal cortisol secretion and ACTH-immunopositive staining without autonomous ACTH secretion. …
NAID 130004443986