免疫表現型検査
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出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2015/12/03 15:59:10」(JST)
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Immunophenotyping is a technique used to study the protein expressed by cells. This technique is commonly used in basic science research and laboratory diagnostic purpose. This can be done on tissue section (fresh or fixed tissue), cell suspension, etc. An example is the detection of tumor marker, such as in the diagnosis of leukemia. It involves the labelling of white blood cells with antibodies directed against surface proteins on their membrane. By choosing appropriate antibodies, the differentiation of leukemic cells can be accurately determined. The labelled cells are processed in a flow cytometer, a laser-based instrument capable of analyzing thousands of cells per second. The whole procedure can be performed on cells from the blood, bone marrow or spinal fluid in a matter of a few hours.
An example of information provided through Immunophenotyping: "The flow cytometric immunophenotyping report indicated the malignant cells were positive for CD19, CD10, dimCD20, CD45, HLA-DR, and λ immunoglobulin light chain. There was no coexpression of CD5 or CD23 by the monoclonal B-cell population."
External links
- British Society for Haematology guidelines accessed July 31, 2006
- Immunophenotyping Lymphomas, University of Medicine & Dentistry of New Jersey
- Immunophenotyping at the US National Library of Medicine Medical Subject Headings (MeSH)
UpToDate Contents
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English Journal
- A retrospective study to assess the relative value of peripheral blood, bone marrow aspirate and biopsy morphology, immunohistochemical stains, and flow cytometric analysis in the diagnosis of chronic B cell lymphoproliferative neoplasms.
- Zhang QY1, Chabot-Richards D, Evans M, Spengel K, Andrews J, Kang H, Foucar K.
- International journal of laboratory hematology.Int J Lab Hematol.2015 Jun;37(3):390-402. doi: 10.1111/ijlh.12299. Epub 2014 Oct 13.
- INTRODUCTION: The successful diagnosis of chronic B cell lymphoproliferative neoplasms (B-CLPN) requires the integration of multiple parameters, beginning with clinical information, CBC data, and morphology review. Immunophenotyping is essential and genetic testing may also be necessary. However, th
- PMID 25307888
- Absence of CD9 expression in acute myeloid leukemia: possible correlation with t(8;21).
- Gupta SK1, Chopra A, Singh S, Kumar R, Bakhshi S, Kumar L, Sharma A.
- International journal of laboratory hematology.Int J Lab Hematol.2015 Jun;37(3):e56-8. doi: 10.1111/ijlh.12296. Epub 2014 Sep 26.
- PMID 25263263
- Detection of central nervous system involvement in childhood acute lymphoblastic leukemia by cytomorphology and flow cytometry of the cerebrospinal fluid.
- Ranta S1, Nilsson F, Harila-Saari A, Saft L, Tani E, Söderhäll S, Porwit A, Hultdin M, Noren-Nyström U, Heyman M.
- Pediatric blood & cancer.Pediatr Blood Cancer.2015 Jun;62(6):951-6. doi: 10.1002/pbc.25363. Epub 2014 Dec 24.
- BACKGROUND: Therapy directed at the central nervous system (CNS) is an essential part of the treatment for childhood acute lymphoblastic leukemia (ALL). The current evaluation of CNS involvement based on cytomorphological examination of the cerebrospinal fluid (CSF) alone is not as sensitive with lo
- PMID 25545289
Japanese Journal
- MDSのimmunophenotyping (MDS(骨髄異形成症候群))
- Wistar Hannoverラットにおける免疫毒性試験に関するデータ
- YAMATOYA Hideyuki,KAWAGUCHI Hiroaki,FUKUDA Takeshi,KADOKURA Hidetomi,YAMASHITA Rie,YOSHIKAWA Tsuyoshi,SHIRAISHI Mitsuya,MIYAMOTO Atsushi,MIYOSHI Noriaki
- Experimental animals 61(2), 171-175, 2012-04-01
- … The immunological data noted for leukocyte parameters, the serum globulin concentration, and immunophenotyping (peripheral blood, spleen, and thymus) relating to chronological changes and sex differences may be useful in assessing drug-related immunotoxicity in this strain. …
- NAID 10030556000
- A case of enteropathy-associated T-cell lymphoma : diagnosis by flow cytometric immunophenotyping and genome analysis using ascitic fluid
- NISHIMURA Masako,TOMO Kenjiro
- International journal of clinical oncology 16(6), 778-782, 2011-12-01
- NAID 10030247807
Related Links
- "In this golden age of biology, we look forward to supporting you on your quest for discovery. Good luck with your research." JONATHAN MILNER, CEO Find out more ... Immunophenotyping Immunophenotyping is the analysis of ...
- Immunophenotyping: Lymphoid cell maturation pathway card Download a PDF of our Lymphoid cell maturation pathway card or request a hard copy today! Download a PDF version or email us to receive your free hard copy today. . ...
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