immunophenotype

免疫表現型

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2014/10/17 15:54:29」(JST)

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Immunophenotyping is a technique used to study the protein expressed by cells. This technique is commonly used in basic science research and laboratory diagnostic purpose. This can be done on tissue section (fresh or fixed tissue), cell suspension, etc. An example is the detection of tumor marker, such as in the diagnosis of leukemia. It involves the labelling of white blood cells with antibodies directed against surface proteins on their membrane. By choosing appropriate antibodies, the differentiation of leukemic cells can be accurately determined. The labelled cells are processed in a flow cytometer, a laser-based instrument capable of analyzing thousands of cells per second. The whole procedure can be performed on cells from the blood, bone marrow or spinal fluid in a matter of a few hours.

An example of information provided through Immunophenotyping: "The flow cytometric immunophenotyping report indicated the malignant cells were positive for CD19, CD10, dimCD20, CD45, HLA-DR, and λ immunoglobulin light chain. There was no coexpression of CD5 or CD23 by the monoclonal B-cell population."

External links

• British Society for Haematology guidelines accessed July 31, 2006
• Immunophenotyping Lymphomas, University of Medicine & Dentistry of New Jersey
• Immunophenotyping at the US National Library of Medicine Medical Subject Headings (MeSH)

UpToDate Contents

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• 1. 造血腫瘍の分類 classification of the hematopoietic neoplasms
• 2. 非ホジキンリンパ腫の臨床症状および診断 clinical presentation and diagnosis of non hodgkin lymphoma
• 3. リンパ球増加症またはリンパ球減少症を有する患者へのアプローチ approach to the patient with lymphocytosis or lymphocytopenia
• 4. 脾辺縁帯リンパ腫の臨床症状、病理学的特徴、および診断 clinical manifestations pathologic features and diagnosis of splenic marginal zone lymphoma
• 5. Sézary症候群の臨床症状、病理学的特徴、および診断 clinical presentation pathologic features and diagnosis of sezary syndrome

English Journal

• Treatment of childhood acute lymphoblastic leukemia in central America: A lower-middle income countries experience.

• Navarrete M1, Rossi E, Brivio E, Carrillo JM, Bonilla M, Vasquez R, Peña A, Fu L, Martinez R, Espinoza CM, Lacayo LF, Rodriguez H, Batista R, Barr R, Howard SC, Ribeiro RC, Masera G, Biondi A, Conter V, Valsecchi MG.Author information 1Department of Hematology, Hospital Nacional de Niños "Dr. Carlos Sáenz Herrera,", San José, Costa Rica.AbstractBACKGROUND: Five Asociación de Hemato-Oncología de Centroamérica (AHOPCA) countries have used an adapted BFM-based protocol for childhood acute lymphoblastic leukemia (ALL).
• Pediatric blood & cancer.Pediatr Blood Cancer.2014 May;61(5):803-9. doi: 10.1002/pbc.24911. Epub 2013 Dec 24.
• BACKGROUND: Five Asociación de Hemato-Oncología de Centroamérica (AHOPCA) countries have used an adapted BFM-based protocol for childhood acute lymphoblastic leukemia (ALL).PROCEDURE: In the AHOPCA-ALL 2008 protocol, patients were stratified by age, white blood cell count, immunophenotype, centra
• PMID 24376115

• Epstein-Barr virus encoded RNA detected by in situ hybridization using cytological preparations.

• Garady C1, Saieg MA, Ko HM, Geddie WR, Boerner SL, da Cunha Santos G.Author information 1Laboratory Medicine Program, University Health Network, Toronto, ON, Canada.AbstractOBJECTIVE: Detection of Epstein-Barr virus (EBV) status might help in the diagnosis of EBV-related neoplasms. The rate of successful assays for the detection of EBV-infected cells in cytological preparations has not been fully explored. Our aims were to examine the rate of successful in situ hybridization (ISH) assays for EBV-encoded RNA (EBER) in cytological specimens and to explore reasons for failure.
• Cytopathology : official journal of the British Society for Clinical Cytology.Cytopathology.2014 Apr;25(2):101-7. doi: 10.1111/cyt.12073. Epub 2013 Jun 3.
• OBJECTIVE: Detection of Epstein-Barr virus (EBV) status might help in the diagnosis of EBV-related neoplasms. The rate of successful assays for the detection of EBV-infected cells in cytological preparations has not been fully explored. Our aims were to examine the rate of successful in situ hybridi
• PMID 23725487

• CD4(+)and CD8(+)T-cell reactions against leukemia-associated- or minor-histocompatibility-antigens in AML-patients after allogeneic SCT.

• Steger B1, Milosevic S2, Doessinger G3, Reuther S4, Liepert A5, Braeu M2, Schick J5, Vogt V5, Schuster F4, Kroell T5, Busch DH6, Borkhardt A4, Kolb HJ1, Tischer J5, Buhmann R1, Schmetzer H7.Author information 1Helmholtz Center Munich (German Research Center for Environmental Health and Clinical Cooperative Group Hematopoetic Cell-Transplantation), 81377 Munich, Germany; University Hospital of Munich, Department for Hematopoetic Cell Transplantation, Med. Dept. 3, 81377 Munich, Germany.2Helmholtz Center Munich (German Research Center for Environmental Health and Clinical Cooperative Group Hematopoetic Cell-Transplantation), 81377 Munich, Germany.3Institute for Medical Microbiology, Immunology and Hygiene, and Focus Group'Clinical Cell Processing and Purification', Institute for Advanced Study, Technical University Munich, 81675 Munich, Germany.4Department of Pediatric Oncology, Hematology and Immunology, University Hospital Duesseldorf, 40225 Duesseldorf, Germany.5University Hospital of Munich, Department for Hematopoetic Cell Transplantation, Med. Dept. 3, 81377 Munich, Germany.6Institute for Medical Microbiology, Immunology and Hygiene, and Focus Group'Clinical Cell Processing and Purification', Institute for Advanced Study, Technical University Munich, 81675 Munich, Germany; Clinical Cooperation Groups "Antigen-specific Immunotherapy" and "Immune Monitoring", Helmholtz Center Munich and Technical University Munich, 81675 Munich, Germany.7Helmholtz Center Munich (German Research Center for Environmental Health and Clinical Cooperative Group Hematopoetic Cell-Transplantation), 81377 Munich, Germany; University Hospital of Munich, Department for Hematopoetic Cell Transplantation, Med. Dept. 3, 81377 Munich, Germany. Electronic address: Helga.Schmetzer@med.uni-muenchen.de.AbstractT-cells play an important role in the remission-maintenance in AML-patients (pts) after SCT, however the role of LAA- (WT1, PR1, PRAME) or minor-histocompatibility (mHag, HA1) antigen-specific CD4(+) and CD8(+)T-cells is not defined. A LAA/HA1-peptide/protein stimulation, cloning and monitoring strategy for specific CD8(+)/CD4(+)T-cells in AML-pts after SCT is given. Our results show that (1) LAA-peptide-specific CD8+T-cells are detectable in every AML-pt after SCT. CD8(+)T-cells, recognizing two different antigens detectable in 5 of 7 cases correlate with long-lasting remissions. Clonal TCR-Vβ-restriction exemplarily proven by spectratyping in PRAME-specific CD8(+)T-cells; high PRAME-peptide-reactivity was CD4(+)-associated, as shown by IFN-γ-release. (2) Two types of antigen-presenting cells (APCs) were tested for presentation of LAA/HA1-proteins to CD4(+)T-cells: miniEBV-transduced lymphoblastoid cells (B-cell-source) and CD4-depleted MNC (source for B-cell/monocyte/DC). We provide a refined cloning-system for proliferating, CD40L(+)CD4(+)T-cells after LAA/HA1-stimulation. CD4(+)T-cells produced cytokines (GM-CSF, IFN-γ) upon exposure to LAA/HA1-stimulation until after at least 7 restimulations and demonstrated cytotoxic activity against naive blasts, but not fibroblasts. Antileukemic activity of unstimulated, stimulated or cloned CD4(+)T-cells correlated with defined T-cell-subtypes and the clinical course of the disease. In conclusion we provide immunological tools to enrich and monitor LAA/HA1-CD4(+)- and CD8(+)T-cells in AML-pts after SCT and generate data with relevant prognostic value. We were able to demonstrate the presence of LAA-peptide-specific CD8(+)T-cell clones in AML-pts after SCT. In addition, we were also able to enrich specific antileukemic reactive CD4(+)T-cells without GvH-reactivity upon repeated LAA/HA1-protein stimulation and limiting dilution cloning.
• Immunobiology.Immunobiology.2014 Apr;219(4):247-60. doi: 10.1016/j.imbio.2013.10.008. Epub 2013 Oct 27.
• T-cells play an important role in the remission-maintenance in AML-patients (pts) after SCT, however the role of LAA- (WT1, PR1, PRAME) or minor-histocompatibility (mHag, HA1) antigen-specific CD4(+) and CD8(+)T-cells is not defined. A LAA/HA1-peptide/protein stimulation, cloning and monitoring stra
• PMID 24315637

Japanese Journal

• フローサイトメトリー (特集 リンパ腫 : 検査・診断と治療の最新動向) -- (リンパ腫の検査・診断)

• 中峯 寛和,倉本 智津子,山口 直子
• 日本臨床 72(3), 412-417, 2014-03
• NAID 40019986337

• 成人ALLの予後因子 (特集 急性リンパ性白血病(ALL))

• 八田 善弘
• 血液内科 68(2), 147-153, 2014-02
• NAID 40020006762

• 小腸に発症したt(11;18)(q21;q21)/BIRC3-MALT1陽性節外性マージナルゾーンリンパ腫／MALT リンパ腫の1 例

• 福島 正大,飯岡 大,宮島 真治,本田 浩太郎,中川 美穂,奥村 敦子,本庄 原,大野 仁嗣
• 天理医学紀要 17(2), 81-89, 2014
• 症例: 78歳女性．多量下血をきたし緊急入院．CTで近位回腸に全周性の壁肥厚と周囲リンパ節の腫大を認めた．シングルバルーン小腸内視鏡で近位回腸に出血を伴う不整な潰瘍病変を認めた．開腹小腸切除術及び腸間膜リンパ節切除術を実施した．切除した小腸の病理標本では, 中型のセントロサイト様細胞や単球様細胞が反応性濾胞の辺縁に増生し, これらのリンパ腫細胞は小腸壁全層および周囲脂肪織に浸潤していた．フローサイ …
• NAID 130004940439

Related Links

• FAB typeとImmunophenotype - 生物学 - 教えて！goo

ヒトの体にはL1、L2、L3といった種類があるらしいのですが、FAB typeとはいったいなんなんでしょうか？血液型みたいなものなのでしょうか？またImmunophenotype（免疫表現型)というものに、「B lineage」と「T li...

• immunophenotype - definition of immunophenotype in the ...

forum Join the Word of the Day Mailing List For webmasters TheFreeDictionary Google Bing? Word / Article Starts with Ends with Text ... immunophenotype /im·mu·no·phe·no·type/ (-fe´no-tīp) a phenotype of cells of hematopoietic ...

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