亜硫酸水素還元酵素、亜硫酸水素レダクターゼ、重亜硫酸還元酵素、重亜硫酸レダクターゼ

WordNet

an enzyme that catalyses the biochemical reduction of some specified substance

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/02/02 10:03:45」(JST)

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In enzymology, a hydrogensulfite reductase (EC 1.8.99.3) is an enzyme that catalyzes the chemical reaction

trithionate + acceptor + 2 H₂O + OH- 3 bisulfite + reduced acceptor

The 4 substrates of this enzyme are trithionate, acceptor, H₂O, and OH-, whereas its two products are bisulfite and reduced acceptor.

This enzyme belongs to the family of oxidoreductases, specifically those acting on a sulfur group of donors with other acceptors. The systematic name of this enzyme class is trithionate:acceptor oxidoreductase. Other names in common use include bisulfite reductase, dissimilatory sulfite reductase, desulfoviridin, desulforubidin, desulfofuscidin, dissimilatory-type sulfite reductase, and trithionate:(acceptor) oxidoreductase. It has 4 cofactors: iron, sulfur, siroheme, and iron-sulfur.

References

Hatchikian EC, Zeikus JG (1983). "Characterization of a new type of dissimilatory sulfite reductase present in Thermodesulfobacterium commune". J. Bacteriol. 153 (3): 1211–20. PMC 221765. PMID 6826522.
Lee JP, Yi CS, LeGall J, Peck HD Jr (1973). "Isolation of a new pigment, desulforubidin, from Desulfovibrio desulfuricans (Norway strain) and its role in sulfite reduction". J. Bacteriol. 115 (1): 453–5. PMC 246260. PMID 4717523.
Trudinger PA (1970). "Carbon monoxide-reacting pigment from Desulfotomaculum nigrificans and its possible relevance to sulfite reduction". J. Bacteriol. 104 (1): 158–70. PMC 248196. PMID 5473884.

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English Journal

Application of denaturing high-performance liquid chromatography for monitoring sulfate-reducing bacteria in oil fields.

Priha O1, Nyyssönen M, Bomberg M, Laitila A, Simell J, Kapanen A, Juvonen R.Author information 1VTT Technical Research Centre of Finland, Espoo, Finland. outi.priha@vtt.fiAbstractSulfate-reducing bacteria (SRB) participate in microbially induced corrosion (MIC) of equipment and H2S-driven reservoir souring in oil field sites. Successful management of industrial processes requires methods that allow robust monitoring of microbial communities. This study investigated the applicability of denaturing high-performance liquid chromatography (DHPLC) targeting the dissimilatory sulfite reductase ß-subunit (dsrB) gene for monitoring SRB communities in oil field samples from the North Sea, the United States, and Brazil. Fifteen of the 28 screened samples gave a positive result in real-time PCR assays, containing 9 × 10(1) to 6 × 10(5) dsrB gene copies ml(-1). DHPLC and denaturing gradient gel electrophoresis (DGGE) community profiles of the PCR-positive samples shared an overall similarity; both methods revealed the same samples to have the lowest and highest diversity. The SRB communities were diverse, and different dsrB compositions were detected at different geographical locations. The identified dsrB gene sequences belonged to several phylogenetic groups, such as Desulfovibrio, Desulfococcus, Desulfomicrobium, Desulfobulbus, Desulfotignum, Desulfonatronovibrio, and Desulfonauticus. DHPLC showed an advantage over DGGE in that the community profiles were very reproducible from run to run, and the resolved gene fragments could be collected using an automated fraction collector and sequenced without a further purification step. DGGE, on the other hand, included casting of gradient gels, and several rounds of rerunning, excising, and reamplification of bands were needed for successful sequencing. In summary, DHPLC proved to be a suitable tool for routine monitoring of the diversity of SRB communities in oil field samples.
Applied and environmental microbiology.Appl Environ Microbiol.2013 Sep;79(17):5186-96. doi: 10.1128/AEM.01015-13. Epub 2013 Jun 21.
Sulfate-reducing bacteria (SRB) participate in microbially induced corrosion (MIC) of equipment and H2S-driven reservoir souring in oil field sites. Successful management of industrial processes requires methods that allow robust monitoring of microbial communities. This study investigated the appli
PMID 23793633

Genome-wide transcriptional profiling of the purple sulfur bacterium Allochromatium vinosum DSM 180T during growth on different reduced sulfur compounds.

Weissgerber T1, Dobler N, Polen T, Latus J, Stockdreher Y, Dahl C.Author information 1Institut für Mikrobiologie und Biotechnologie, Rheinische Friedrich-Wilhelms-Universität Bonn, Bonn, Germany.AbstractThe purple sulfur bacterium Allochromatium vinosum DSM 180(T) is one of the best-studied sulfur-oxidizing anoxygenic phototrophic bacteria, and it has been developed into a model organism for laboratory-based studies of oxidative sulfur metabolism. Here, we took advantage of the organism's high metabolic versatility and performed whole-genome transcriptional profiling to investigate the response of A. vinosum cells upon exposure to sulfide, thiosulfate, elemental sulfur, or sulfite compared to photoorganoheterotrophic growth on malate. Differential expression of 1,178 genes was observed, corresponding to 30% of the A. vinosum genome. Relative transcription of 551 genes increased significantly during growth on one of the different sulfur sources, while the relative transcript abundance of 627 genes decreased. A significant number of genes that revealed strongly enhanced relative transcription levels have documented sulfur metabolism-related functions. Among these are the dsr genes, including dsrAB for dissimilatory sulfite reductase, and the sgp genes for the proteins of the sulfur globule envelope, thus confirming former results. In addition, we identified new genes encoding proteins with appropriate subcellular localization and properties to participate in oxidative dissimilatory sulfur metabolism. Those four genes for hypothetical proteins that exhibited the strongest increases of mRNA levels on sulfide and elemental sulfur, respectively, were chosen for inactivation and phenotypic analyses of the respective mutant strains. This approach verified the importance of the encoded proteins for sulfur globule formation during the oxidation of sulfide and thiosulfate and thereby also documented the suitability of comparative transcriptomics for the identification of new sulfur-related genes in anoxygenic phototrophic sulfur bacteria.
Journal of bacteriology.J Bacteriol.2013 Sep;195(18):4231-45. doi: 10.1128/JB.00154-13. Epub 2013 Jul 19.
The purple sulfur bacterium Allochromatium vinosum DSM 180(T) is one of the best-studied sulfur-oxidizing anoxygenic phototrophic bacteria, and it has been developed into a model organism for laboratory-based studies of oxidative sulfur metabolism. Here, we took advantage of the organism's high meta
PMID 23873913

Dominance of green sulfur bacteria in the chemocline of the meromictic Lake Suigetsu, Japan, as revealed by dissimilatory sulfite reductase gene analysis.

Mori Y1, Kataoka T, Okamura T, Kondo R.Author information 1Department of Marine Bioscience, Fukui Prefectural University, Obama, Fukui 917-0003, Japan. s1094001@s.fpu.ac.jpAbstractThis study investigated the spatiotemporal abundance and diversity of the α-subunit of the dissimilatory sulfite reductase gene (dsrA) in the meromictic Lake Suigetsu for assessing the sulfur-oxidizing bacterial community. The density of dsrA in the chemocline reached up to 3.1 × 10(6) copies ml(-1) in summer by means of quantitative real-time PCR and it was generally higher than deeper layers. Most of the dsrA clones sequenced were related to green sulfur bacteria such as Chlorobium phaeovibrioides, C. limicola, and C. luteolum. Below the chemocline of the lake, we also detected other dsrA clones related to the purple sulfur bacterium Halochromatium salexigens and some branching lineages of diverse sequences that were related to chemotrophic sulfur bacterial species such as Magnetospirillum gryphiswaldense, Candidatus Ruthia magnifica, and Candidatus Thiobios zoothamnicoli. The abundance and community compositions of sulfur-oxidizing bacteria changed depending on the water depth and season. This study indicated that the green sulfur bacteria dominated among sulfur-oxidizing bacterial population in the chemocline of Lake Suigetsu and that certain abiotic environmental variables were important factors that determined sulfur bacterial abundance and community structure.
Archives of microbiology.Arch Microbiol.2013 May;195(5):303-12. doi: 10.1007/s00203-013-0879-5. Epub 2013 Mar 3.
This study investigated the spatiotemporal abundance and diversity of the α-subunit of the dissimilatory sulfite reductase gene (dsrA) in the meromictic Lake Suigetsu for assessing the sulfur-oxidizing bacterial community. The density of dsrA in the chemocline reached up to 3.1 × 10(6) copies ml(-
PMID 23455488

★リンクテーブル★

リンク元	「重亜硫酸還元酵素」「亜硫酸水素還元酵素」「重亜硫酸レダクターゼ」「亜硫酸水素レダクターゼ」
関連記事	「reductase」「hydrogensulfite」

「重亜硫酸還元酵素」

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hydrogensulfite reductase
Identifiers
EC number	1.8.99.3
CAS number	9059-42-1
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
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NCBI	proteins