English Journal

Sulphation pattern analysis of chemically sulphated polysaccharide LbGp1 from Lycium barbarum by GC-MS.

Sun Y1, Sun W1, Guo J1, Hu X1, Gong G1, Huang L1, Cao H2, Wang Z3.
Food chemistry.Food Chem.2015 Mar 1;170:22-9. doi: 10.1016/j.foodchem.2014.08.024. Epub 2014 Aug 17.
The polysaccharide LbGp1 from Lycium barbarum L. was sulphated with sulphur trioxide-pyridine complex in DMF, yielding two sulphated polysaccharides, which were LbGp1-OL-SL with 13.7% sulphate content, and LbGp1-OL-SH with 27.4% sulphate content. The sulphation patterns were analysed using a GC-MS s
PMID 25306313

Piirainen MA1, de Ruijter JC1, Koskela EV1, Frey AD2.
New biotechnology.N Biotechnol.2014 Dec 25;31(6):532-7. doi: 10.1016/j.nbt.2014.03.001. Epub 2014 Mar 13.
N-linked glycosylation of proteins is one of the most common posttranslational modifications. N-glycan structures and N-glycosylation efficiency are crucial parameters in the production of N-glycosylated proteins. Yeast cells can be seen as an attractive production host for therapeutic glycoproteins
PMID 24632452

Proteolytic and N-Glycan Processing of Human Alpha-1 Antitrypsin Expressed in Nicotiana benthamiana.

Castilho A1, Windwarder M2, Gattinger P3, Mach L4, Strasser R4, Altmann F5, Steinkellner H6.
Plant physiology.Plant Physiol.2014 Oct 29. pii: pp.114.250720. [Epub ahead of print]
Plants are increasingly being used as an expression system for complex recombinant proteins. However, our limited knowledge of the intrinsic factors that act along the secretory pathway, which may compromise product integrity, renders process design difficult in some cases. Here, we pursued the reco
PMID 25355867

Japanese Journal

N-terminal vacuolar sorting signal at the mouse antibody alters the N-linked glycosylation pattern in suspension-cultured tobacco BY2 cells(PLANT BIOTECHNOLOGY)

Misaki Ryo,Sakai Yohei,Omasa Takeshi [他],Fujiyama Kazuhito,Seki Tatsuji
Journal of bioscience and bioengineering 112(5), 476-484, 2011-11
… However, because glycosylation in plants is different from that in human cells, the development of glycoengineering is required. …
NAID 110008761512