Plasmodium gallinaceum

English Journal

Loop-mediated isothermal amplification assay for detection of Histomonas meleagridis infection in chickens targeting the 18S rRNA sequences.

Xu J, Qu C, Tao J.Author information a Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou , PR China.AbstractHistomonas meleagridis is the causative agent of histomonosis, a disease of gallinaceous fowl characterized by necrotic typhlitis, hepatitis, and high mortality. To develop a rapid and sensitive method for specific detection of H. meleagridis, an assay based on loop-mediated isothermal amplification (LAMP) targeting the 18S rRNA gene was established. The detection limit of the LAMP assay was 10 copies for standard plasmids containing an 18S rRNA gene fragment, which was superior to that of a classical PCR method. Specificity tests revealed that there was no cross-reaction with other protozoa such as Trichomonas gallinae, Blastocytis sp, Tetratrichomonas gallinarum, Plasmodium gallinaceum, Toxoplasma gondii, Eimeria tenella, Leucocytozoon caulleryi and Leucocytozoon sabrazesi. The assay was evaluated for its diagnostic utility using liver and caeca samples collected from suspected field cases, the detection rate was 100 and 97.92%, respectively. These results indicate that the LAMP assay may be a useful tool for rapid detection and identification of H. meleagridis in poultry.
Avian pathology : journal of the W.V.P.A.Avian Pathol.2014 Jan 23. [Epub ahead of print]
Histomonas meleagridis is the causative agent of histomonosis, a disease of gallinaceous fowl characterized by necrotic typhlitis, hepatitis, and high mortality. To develop a rapid and sensitive method for specific detection of H. meleagridis, an assay based on loop-mediated isothermal amplification
PMID 24320623

Loop-mediated isothermal amplification assay for detection of Histomonas meleagridis infection in chickens targeting the 18S rRNA sequences.

Xu J, Qu C, Tao J.Author information a Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou , PR China.AbstractHistomonas meleagridis is the causative agent of histomonosis, a disease of gallinaceous fowl characterized by necrotic typhlitis, hepatitis, and high mortality. To develop a rapid and sensitive method for specific detection of H. meleagridis, an assay based on loop-mediated isothermal amplification (LAMP) targeting the 18S rRNA gene was established. The detection limit of the LAMP assay was 10 copies for standard plasmids containing an 18S rRNA gene fragment, which was superior to that of a classical PCR method. Specificity tests revealed that there was no cross-reaction with other protozoa such as Trichomonas gallinae, Blastocytis sp, Tetratrichomonas gallinarum, Plasmodium gallinaceum, Toxoplasma gondii, Eimeria tenella, Leucocytozoon caulleryi, and Leucocytozoon sabrazesi. The assay was evaluated for its diagnostic utility using field liver and caeca samples collected from suspected cases, the detection rate was 100 and 97.92%, respectively. These results indicate that the LAMP assay may be a useful tool for rapid detection and identification of H. meleagridis in poultry.
Avian pathology : journal of the W.V.P.A.Avian Pathol.2013 Dec 11. [Epub ahead of print]
Histomonas meleagridis is the causative agent of histomonosis, a disease of gallinaceous fowl characterized by necrotic typhlitis, hepatitis, and high mortality. To develop a rapid and sensitive method for specific detection of H. meleagridis, an assay based on loop-mediated isothermal amplification
PMID 24325302

In vivo transmission blocking activities of artesunate on the avian malaria parasite Plasmodium gallinaceum.

Kumnuan R, Pattaradilokrat S, Chumpolbanchorn K, Pimnon S, Narkpinit S, Harnyuttanakorn P, Saiwichai T.Author information Department of Parasitology and Entomology, Faculty of Public Health, Mahidol University, Bangkok 10400, Thailand.AbstractInfection and transmission of the avian malaria parasite Plasmodium gallinaceum in domestic chickens is associated with high economic burden and presents a major challenge to poultry industry in South East Asia. Development of drugs targeting both asexual blood stage parasites and sexual stages of the avian malarias will be beneficial for malaria treatment and eradication. However, current drugs recommended for treatment of the avian malaria parasites target specifically the asexual blood stage parasites, but have little or no impact to the gametocytes, the major target for development of transmission-blocking strategies. In the present work, we established a simple procedure to evaluate gametocytocidal and transmission blocking activities in a P. gallinaceum-avian model. The assays involved administration of seven consecutive daily doses of test compounds into P. gallinaceum-infected chickens with 10% parasitaemia and 1% gametocytaemia. Our studies indicated that intramuscular injection with seven daily low doses (the minimum effective dose of 10mg/kg) of artesunate blocked the gametocyte production and transmission to the mosquito vector Aedes aegypti. This assay can be further applicable for testing new compounds against P. gallinaceum and for other parasitic protozoa infecting birds.
Veterinary parasitology.Vet Parasitol.2013 Nov 8;197(3-4):447-54. doi: 10.1016/j.vetpar.2013.07.024. Epub 2013 Jul 22.
Infection and transmission of the avian malaria parasite Plasmodium gallinaceum in domestic chickens is associated with high economic burden and presents a major challenge to poultry industry in South East Asia. Development of drugs targeting both asexual blood stage parasites and sexual stages of t
PMID 23937960

Japanese Journal

南大東島で採集された蚊における鳥マラリア原虫遺伝子の検出(寄生虫病学)

江尻寛子,佐藤雪太,佐々木絵美,炭山大輔,津田良夫,沢辺京子,松井晋,堀江明香,赤谷加奈,高木昌興,大森澄枝,村田浩一,湯川眞嘉
The journal of veterinary medical science 70(11), 1205-1210, 2008-11-25
沖縄県南大東島に生息している鳥類に鳥マラリア原虫感染が見られたため,ベクター探索を目的として蚊を採集して原虫保有の有無について調べた.2006年3月から2007年2月までの間,南大東島各所で1264個体,5属9種の蚊が採集された.蚊の種類,採集時期および捕獲地点ごとに1〜5個体をプールしてDNA抽出を行い,鳥マラリア原虫のcyt b遺伝子を標的としたneseted PCRを行った.ネッタイイエカ( …
NAID 110006987056

鶏マラリア

磯部尚
動物の原虫病 = Journal of animal protozoosis 22(1), 22-30, 2007-12-15
… juxtanucleareとP.gallinaceumの2種である。 … 近年わが国で分離されたP.juxtanucleareおよび保存されていたP.gallinaceumについての病原性試験などを行う機会があったので、その成績を中心に鶏マラリアについて紹介したい。 …
NAID 10025608767