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Impedimetric microbial sensor for real-time monitoring of phage infection of Escherichia coli.

Cheng MS, Ho JS, Lau SH, Chow VT, Toh CS.SourceDivision of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore.
Biosensors & bioelectronics.Biosens Bioelectron.2013 Sep 15;47:340-4. doi: 10.1016/j.bios.2013.03.050. Epub 2013 Mar 30.
We describe an impedimetric microbial sensor for real-time monitoring of the non-lytic M13 bacteriophage infection of Escherichia coli cells using a gold electrode covalently grafted with a monolayer of lipopolysaccharide specific antibody. After infection, damage to the lipopolysaccharide layer on
PMID 23603131

Murata VM, Schmidt MC, Kalil J, Tsuruta LR, Moro AM.SourceLaboratorio de Biofarmacos em Celulas Animais, Instituto Butantan, São Paulo, Brazil.
Molecular biotechnology.Mol Biotechnol.2013 Jun;54(2):269-77. doi: 10.1007/s12033-012-9564-1.
Digoxin is a pharmaceutical used in the control of cardiac dysfunction. Its therapeutic window is narrow, with effect dosage very close to the toxic dosage. To counteract the toxic effect, polyclonal Fab fragments are commercially available. Our study is based on a monoclonal anti-digoxin antibody,
PMID 23359127

Orthogonal Labeling of M13 Minor Capsid Proteins with DNA to Self-Assemble End-to-End Multiphage Structures.

Hess GT, Guimaraes CP, Spooner E, Ploegh HL, Belcher AM.SourceThe David H. Koch Institute for Integrative Cancer Research, ‡Department of Materials Science and Engineering, ∥Department of Biology, and ⊥Department of Biological Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.
ACS synthetic biology.ACS Synth Biol.2013 May 28. [Epub ahead of print]
M13 bacteriophage has been used as a scaffold to organize materials for various applications. Building more complex multiphage devices requires precise control of interactions between the M13 capsid proteins. Toward this end, we engineered a loop structure onto the pIII capsid protein of M13 bacteri
PMID 23713956

Host-parasite relations of bacteria and phages can be unveiled by Oligostickiness, a measure of relaxed sequence similarity

西垣功一
BIOINFORMATICS 25(5), 563-570, 2009-03
… Interestingly, according to SOSS values, lysogenic phages such as lambda phage (host: Escherichia coli) or SPP1 (host: Bacillus subtilis) have distinctively higher similarity with its host than its non-lysogenic (excretive or virulent) ones such as fd and T4 (host: E. …
NAID 120001371424

Extracting Characteristic Properties of Fitness Landscape from In Vitro Molecular Evolution: A Case Study on Infectivity of fd Phage to E.coli.

相田拓洋,伏見譲
JOURNAL OF THEORETICAL BIOLOGY 246(3), 538-550, 2007-06
… Applying our methodology, we analyzed experimental data on the in vitro evolution of a random polypeptide (139 amino acids) toward acquiring infectivity (= ability to infect) of fd phage. …
NAID 120001371225

相田拓洋,伏見譲
PLoS ONE 1(e96), 1-8, 2006
… To plot the fitness landscape of protein function, we carried out in vitro molecular evolution beginning with a defective fd phage carrying a random polypeptide of 139 amino acids in place of the g3p minor coat protein D2 domain, which is essential for phage infection. …
NAID 120001371221