WordNet

a red fluorescent dye resulting from the action of bromine on fluorescein; used in cosmetics and as a biological stain for studying cell structures (同)bromeosin
of or relating to eosinophil

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2018/09/10 13:39:58」(JST)

Eosin is the name of several fluorescent acidic compounds which bind to and form salts with basic, or eosinophilic, compounds like proteins containing amino acid residues such as arginine and lysine, and stains them dark red or pink as a result of the actions of bromine on fluorescein. In addition to staining proteins in the cytoplasm, it can be used to stain collagen and muscle fibers for examination under the microscope. Structures that stain readily with eosin are termed eosinophilic.

Etymology

The name Eosin comes from Eos, the Ancient Greek word for 'dawn' and the name of the Ancient Greek goddess of the dawn.

Variants

There are actually two very closely related compounds commonly referred to as eosin. Most often used is Eosin Y (also known as eosin Y ws, eosin yellowish, Acid Red 87, C.I. 45380, bromoeosine, bromofluoresceic acid, D&C Red No. 22); it has a very slightly yellowish cast. The other eosin compound is eosin B (eosin bluish, Acid Red 91, C.I. 45400, Saffrosine, Eosin Scarlet, or imperial red); it has a very faint bluish cast. The two dyes are interchangeable, and the use of one or the other is a matter of preference and tradition.

Eosin Y is a tetrabromo derivative of fluorescein.^[1] Eosin B is a dibromo dinitro derivative of fluorescein.^[2]

Uses

Use in histology

Sample of a trachea coloured with hematoxylin and eosin.

Eosin is most often used as a counterstain to hematoxylin in H&E (haematoxylin and eosin) staining. H&E staining is one of the most commonly used techniques in histology. Tissue stained with haematoxylin and eosin shows cytoplasm stained pink-orange and nuclei stained darkly, either blue or purple. Eosin also stains red blood cells intensely red.

For staining, eosin Y is typically used in concentrations of 1 to 5 percent weight by volume, dissolved in water or ethanol.^[3] For prevention of mold growth in aqueous solutions, thymol is sometimes added.^[4] A small concentration (0.5 percent) of acetic acid usually gives a deeper red stain to the tissue.

It is listed as an IARC class 3 carcinogen.

Other uses

Eosin is also used as a red dye in ballpoint pen inks.

References

Jocelyn H. Bruce-Gregorios, M.D.: Histopathologic Techniques, JMC Press Inc., Quezon City, Philippines, 1974.

^ Its CAS number is 17372-87-1 and its SMILES structure is O=C5C(Br)=C2O C1=C(Br)C([O-]) =C(Br)C=C1C(C4=C (C([O-])=O)C=C C=C4)=C2C=C3Br.
^ Its CAS number is 548-28-3 and its SMILES structure is O=C5C(Br)=C2O C1=C(Br)C([O-]) =C([N+]([O-])=O) C=C1C(C4=C(C([O-]) =O)C=CC=C4)=C2 C=C3[N+]([O-])=O.
^ "Haematoxylin Eosin (H&E) staining". protocolsonline.com. 11 April 2010. Retrieved 22 April 2018.
^ Hitokoto H, Morozumi S, Wauke T, Sakai S, Kurata H (1980). "Inhibitory effects of spices on growth and toxin production of toxigenic fungi". Appl. Environ. Microbiol. 39: 818–22. PMC 291425 . PMID 6769391.

External links

Eosin Y - Applications information

UpToDate Contents

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English Journal

Boric acid-enhanced embedding medium for cryomicrotomy.

Lim JI, Park HK.SourceDepartment of Biomedical Engineering and Healthcare Industry Research Institute, College of Medicine, Kyung Hee University, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
Acta histochemica.Acta Histochem.2012 May;114(3):296-9. Epub 2011 May 13.
A polyvinyl alcohol (PVA)/polyethylene glycol (PEG)-based resin is commonly used as a cryoembedding medium for the histological analysis of frozen tissue sections. However, it is not easy to obtain sufficient numbers of satisfactory reproducible sections owing to the differences between the mechanic
PMID 21570708

Oxymatrine attenuates bleomycin-induced pulmonary fibrosis in mice via the inhibition of inducible nitric oxide synthase expression and the TGF-β/Smad signaling pathway.

Liu L, Lu W, Ma Z, Li Z.SourceInstitute of Respiratory Diseases, The First Affiliated Hospital of China Medical University, Shenyang 110001, P.R. China.
International journal of molecular medicine.Int J Mol Med.2012 May;29(5):815-22. doi: 10.3892/ijmm.2012.923. Epub 2012 Feb 21.
Oxymatrine (OM) is an alkaloid extracted from the Chinese herb Sophora flavescens Ait. with a variety of pharmacological activities. The aim of this study was to investigate the preventive effects of OM on bleomycin (BLM)-induced pulmonary fibrosis (PF) and to fur
PMID 22367596

Japanese Journal

Correlation of histopathology with magnetic resonance imaging in Kienböck disease.

Ogawa Takeshi,Nishiura Yasumasa,Hara Yuki,Okamoto Yoshikazu,Ochiai Naoyuki,小川健,西浦康正,原友紀,岡本嘉一,落合直之
The journal of hand surgery 37(1), 83-89, 2012-01
… We stained paraffin-embedded, coronally sectioned specimens with hematoxylin-eosin and compared them with presurgical coronal scans using MRI with a 47-mm microscopy surface coil.RESULTS:Toward the center of the lunates, the signal intensity in the proton density-weighted images was reduced, whereas the dorsal and palmar sides of the lunates exhibited no changes in intensity. …
NAID 120003825061

Cold-shock eliminates female nucleus in fertilized eggs to induce androgenesis in the loach (Misgurnus anguillicaudatus), a teleost fish.

Morishima Kagayaki,Fujimoto Takafumi,Sato Mami,Kawae Ayako,Zhao Yan,Yamaha Etsuro,Arai Katsutoshi
BMC Biotechnology 11(1), 116, 2011-11-29
… Nuclear behavior after the cold-shock treatment was traced by microscopic observation of DAPI (4'6-diamidino-2-phenylindole)-stained samples and hematoxylin-eosin stained histological sections, and the extrusion of egg (maternal) nucleus was observed in eggs treated in the optimum timing. …
NAID 120003702855

Related Pictures

Eosin Y 1% Aaqueous » Novogen – Patoloji, Histoloji, Sitoloji Eosin Y Stain, 30mL (1 oz) Eosin G Stain, 2% solution | Labstock Microservices Eosin Y 1% Alcoholic 1Gal. - Avantik Biogroup Al'Asi Group - HYDROUS EOSIN 2% 60ml Eosin Y, C.I. 45380, certified | Polysciences, Inc. Mercurochrome Eosin 2% 100ml H&E Staining Agents - Harris‘ hematoxylin solution, Mayer’s hemalum solution & 0.2

★リンクテーブル★

リンク元	「エオシン」
拡張検索	「acute eosinophilic pneumonia」「eosinophil granule protein」「eosinophilic endomyocardial disease」「eosinophilic pustular dermatosis」

「エオシン」

エオシンB
	IUPAC名 4',5'-dibromo-3',6'-dihydroxy-2',7'-dinitro-1-spiro[isobenzofuran-3,9'-xanthene]one
識別情報
CAS登録番号	548-24-3
PubChem	452704
MeSH	Eosine+I+Bluish
	SMILES C1=CC=C2C(=C1)C(=O)OC23C4=CC(=C(C(=C4OC5=C(C(=C(C=C35)[N+](=O)[O-])O)Br)Br)O)[N+](=O)[O-];
特性
化学式	C20H8Br2N2O9
モル質量	580.09352
	特記なき場合、データは常温 (25 °C)・常圧 (100 kPa) におけるものである。

エオシンY
	IUPAC名 2-(2,4,5,7-tetrabromo-6-oxido-3-oxo-3H-xanthen-9-yl)benzoate [in its deprotonated form]
識別情報
CAS登録番号	17372-87-1
PubChem	27020
MeSH	Eosine+Yellowish-(YS)
	SMILES C1=CC=C2C(=C1)C(=O)OC23C4=CC(=C(C(=C4OC5=C(C(=C(C=C35)Br)O)Br)Br)O)Br;
特性
化学式	C20H8Br4O5
モル質量	647.89052
	特記なき場合、データは常温 (25 °C)・常圧 (100 kPa) におけるものである。

　　[★]

英: eosin
同: エオジン ←　エオシン　と呼ぶ人が多いみたい。
関: 染色法

酸性色素
正電荷を帯びた部位を赤く染める

例えば、表皮の角層はエオジンで赤く染まり、リゾチームを多く含むパネート細胞はエオジンで赤く染まる。

エオシン-Y、マーキュクロムは-COONaとう側鎖を有する。

「acute eosinophilic pneumonia」

　　[★] 急性好酸球性肺炎、AEP

「eosinophil granule protein」

　　[★]

好酸球顆粒タンパク質

「eosinophilic endomyocardial disease」

　　[★] 好酸性心内膜心筋疾患

「eosinophilic pustular dermatosis」

　　[★] 好酸球性膿疱性皮膚症

[1] Its CAS number is 17372-87-1 and its SMILES structure is O=C5C(Br)=C2O C1=C(Br)C([O-]) =C(Br)C=C1C(C4=C (C([O-])=O)C=C C=C4)=C2C=C3Br.

[2] Its CAS number is 548-28-3 and its SMILES structure is O=C5C(Br)=C2O C1=C(Br)C([O-]) =C([N+]([O-])=O) C=C1C(C4=C(C([O-]) =O)C=CC=C4)=C2 C=C3[N+]([O-])=O.

[3] "Haematoxylin Eosin (H&E) staining". protocolsonline.com. 11 April 2010. Retrieved 22 April 2018.

[4] Hitokoto H, Morozumi S, Wauke T, Sakai S, Kurata H (1980). "Inhibitory effects of spices on growth and toxin production of toxigenic fungi". Appl. Environ. Microbiol. 39: 818–22. PMC 291425 . PMID 6769391.

v t e Antiseptics and disinfectants (D08)
Acridine derivatives	Ethacridine lactate 9-Aminoacridine Euflavine
Biguanides and amidines	Dibrompropamidine Chlorhexidine^# Propamidine Hexamidine Polihexanide
Phenol and derivatives	Hexachlorophene Policresulen Phenol Triclosan Triclocarban Chloroxylenol^# Biphenylol Fenticlor
Nitrofuran derivatives	Nitrofurazone
Iodine products	Iodine/octylphenoxypolyglycolether Povidone-iodine^# Diiodohydroxypropane
Quinoline derivatives	Dequalinium Chlorquinaldol Oxyquinoline Clioquinol
Quaternary ammonium compounds	Benzalkonium Benzethonium chloride Cetrimonium (bromide/chloride) Cetylpyridinium Cetrimide Benzoxonium chloride Didecyldimethylammonium chloride
Mercurial products	Mercuric amidochloride Phenylmercuric borate Mercuric chloride Merbromin Thiomersal Mercuric iodide
Silver compounds	Silver nitrate
Alcohols	Propanol (propyl alcohol) Isopropanol (isopropyl alcohol) Ethanol (ethyl alcohol)^#
Other	Potassium permanganate Sodium hypochlorite Hydrogen peroxide Eosin Tosylchloramide Octenidine dihydrochloride
^#WHO-EM ^‡Withdrawn from market Clinical trials: ^†Phase III ^§Never to phase III

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eosin