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English Journal

Efficient gene delivery into multiple CNS territories using in utero electroporation.

Dixit R, Lu F, Cantrup R, Gruenig N, Langevin LM, Kurrasch DM, Schuurmans C.SourceDepartment of Biochemistry and Molecular Biology, Hotchkiss Brain Institute, Alberta Children's Hospital Research Institute, University of Calgary.
Journal of visualized experiments : JoVE.J Vis Exp.2011 Jun 23;(52). pii: 2957. doi: 10.3791/2957.
The ability to manipulate gene expression is the cornerstone of modern day experimental embryology, leading to the elucidation of multiple developmental pathways. Several powerful and well established transgenic technologies are available to manipulate gene expression levels in mouse, allowing for t
PMID 21730943

Henslee BE, Morss A, Hu X, Lafyatis GP, Lee LJ.SourceDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio 43210, USA.
Analytical chemistry.Anal Chem.2011 Jun 1;83(11):3998-4003. Epub 2011 May 6.
Electropermeabilization or electroporation is the electrical disruption of a cell's membrane to introduce drugs, DNA/RNA, proteins, or other therapies into the cell. Despite four decades of study, the fundamental science of the process remains poorly understood and controversial. We measured the min
PMID 21473595

A high throughput microelectroporation device to introduce a chimeric antigen receptor to redirect the specificity of human T cells.

Choi Y, Yuen C, Maiti SN, Olivares S, Gibbons H, Huls H, Raphael R, Killian TC, Stark DJ, Lee DA, Torikai H, Monticello D, Kelly SS, Kebriaei P, Champlin RE, Biswal SL, Cooper LJ.SourceDivision of Pediatrics, Children's Cancer Hospital, The University of Texas Graduate School of Biomedical Sciences at Houston, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA.
Biomedical microdevices.Biomed Microdevices.2010 Oct;12(5):855-63.
It has been demonstrated that a chimeric antigen receptor (CAR) can directly recognize the CD19 molecule expressed on the cell surface of B-cell malignancies independent of major histocompatibility complex (MHC). Although T-cell therapy of tumors using CD19-specific CAR is promising, this approach r
PMID 20574820