English Journal

[Morphological and functional heterogeneity of rat ascitic hepatoma Zajdela cells].

Tiuriaeva II, Filatova NA, Rozanov IuM, Demin SIu, Blinova GI, Ivanov VA.AbstractThis work is devoted to the study of molecular and cellular mechanisms of disdifferentiation during neoplastic transformation of cells by investigating the malignant tumor cell heterogeneity. We have revealed two cell fractions of hepatoma Zajdela which differ in patterns of growth in primary culture. The cells of one fraction were attached to the culture plastic and grew in a monolayer (S-fraction), whereas cells of another fraction floated in the culture medium (F-fraction). Using method of lifetime supervision of primary culture cells (1-2 passages) at the limit of the resolving power of DIC-microscopy it has been revealed, that both fractions contain cells of several types. Some of them were specific for one of the fractions, and others were found in both fractions, but their frequencies differed. It has been shown by the same method, that long separate cultivation of these fractions in vitro (more than 50 passage) change both cellular structure and the initial ratio of different types of cells in both fractions. According to DNA flow cytometry, the cells of both fractions were hypotetraploid and had insignificant differences in DNA contents. After adaptation to in vitro conditions, S-fraction cells raised their proliferative activity in comparison with the F-fraction cells, and after long cultivation showed 2.3 times higher DNA content. Greater amount of cell surface laminin, a hepatocellular carcinoma marker, was observed on F-fraction cells than on S-fraction cells. Interfractional distinctions were confirmed also by immunologic assessment of hepatoma cells resistance to natural killer lyses: the sensitivity of S-fraction cells in primary culture was 2.4 times higher than F-fraction cells sensitivity, and, after long cultivation, F-fraction cells became practically resistant to cytotoxic action of natural killers. Based on the data obtained, the most probable paths of cell disdifferentiation during hepatoma Zajdela formation and during long cultivation of this tumor cells in vitro are discussed.
Tsitologiia.Tsitologiia.2010;52(10):817-26.
This work is devoted to the study of molecular and cellular mechanisms of disdifferentiation during neoplastic transformation of cells by investigating the malignant tumor cell heterogeneity. We have revealed two cell fractions of hepatoma Zajdela which differ in patterns of growth in primary cultur
PMID 21268837

[Differential-diagnostic value of changes in nuclear ploidy of prostate epithelial cells during the carcinogenesis process].

Avtandilov GG, Gundorova LV, Saniev KB.AbstractPloidometry of prostatic cell nuclei at different stages of carcinogenesis was made in 80 patients using 8 mcm histological sections stained by Felgen. Ploidy of 1332 nuclei of prostatic epithelial cells and 396 lymphocytes (tissue ploidy standard) was studied on computer image analyser "Imager-CG (computer program version Avtan-San). The study covered interphasic nuclei of the cells of the germinative zones of normal and hyperplastic structures, areas of intraepithelial neoplasia and adenocarcinoma with various disdifferentiation degree. Significant differences in ploidy and proliferative activity of prostatic cells at different carcinogenesis stages were revealed. Ploidometry data may help to take decision in differential histopathological diagnosis of benign and malignant tumors of the prostate.
Urologii͡a (Moscow, Russia : 1999).Urologiia.2002 May-Jun;(3):8-11.
Ploidometry of prostatic cell nuclei at different stages of carcinogenesis was made in 80 patients using 8 mcm histological sections stained by Felgen. Ploidy of 1332 nuclei of prostatic epithelial cells and 396 lymphocytes (tissue ploidy standard) was studied on computer image analyser "Imager-CG (
PMID 12180069

Japanese Journal

神経線維腫症に合併し骨, 軟骨, 筋への異分化を伴った悪性神経鞘腫の1例

井上奈津彦,三原一郎,新村眞人
Ｓｋｉｎ　Ｃａｎｃｅｒ 7(2), 200-203, 1992
The patient was 27-year-old female with neurofibromatosis. She developed a malignant schwannoma on the left femoral area 4 months previously, which was treated with disarticulation of left hip joint. …
NAID 130001079270

LIVER TUMORS INDUCED IN RATS BY 3'-METHYL-4-DIMETHYL AMINOAZOBENZENE III. Electron Microscopic Investigation - Disdifferentiation of Cancer Cells -

Fuse Yusuke,Iwasaki Tomomi,Ono? Tamenori
1969-06-00
NAID 120001796305

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