English Journal

Structure of solid-supported lipid membrane probed by noble metal nanoparticle deposition.

An HH, Lee SJ, Kim HS, Han WB, Yoon CS.AbstractDirect deposition of a noble metal layer onto a solid-supported membrane was proposed as an indirect microscopy tool to visually observe different lipid phases that may develop in the lipid membrane. The method relied on the different permeability of the lipid membrane towards the incident atoms during deposition. Liquid state or structural defects such as phase boundaries, step ledges in a multi-lamellar stack, and pores permitted the metal atoms to penetrate and nucleate inside the membrane whereas rigid gel state was relatively impermeable to the incident atoms, thus enabling visualization of liquid phase or structural defects inside the gel state. Based on the proposed method, we demonstrated the phase states resulting from thermotropic transitions of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), dioleoylphosphatidylethanolamine (DOPE)/DPPC mixture, and 1,2-dioleoyl-3-trimethylammonium propane (DOTAP). Although the proposed method does not allow in-situ observation of equilibrium states, the method should be an excellent complementary tool for visualizing the lipid phases as the method can resolve fine structural details (up to tens of nanometer scale) as seen in the DPPC membrane while providing macroscopic images (up to several micrometers).
Biochimica et biophysica acta.Biochim Biophys Acta.2012 Jul 20. [Epub ahead of print]
Direct deposition of a noble metal layer onto a solid-supported membrane was proposed as an indirect microscopy tool to visually observe different lipid phases that may develop in the lipid membrane. The method relied on the different permeability of the lipid membrane towards the incident atoms dur
PMID 22820148

Effect of Helper Lipids on the Interaction of DNA with Cationic Lipid Monolayers Studied by Specular Neutron Reflection.

Dabkowska AP, Barlow DJ, Campbell RA, Hughes AV, Quinn PJ, Lawrence MJ.SourceInstitute of Pharmaceutical Science, School of Biomedical Sciences, King's College London , 150 Stamford Street, London, SE1 9NH, U.K.
Biomacromolecules.Biomacromolecules.2012 Jul 9. [Epub ahead of print]
The interaction of DNA with monolayers of the cationic lipid dimethyldioctadecylammonium bromide, with/without 50 mol % of a neutral "helper" lipid, either dioleoylphosphatidylethanolamine or cholesterol, has been studied using specular neutron reflection, surface pressure-area isotherms, and Brewst
PMID 22724519

Japanese Journal

Preparation, Physicochemical Properties, and Transfection Activities of Tartaric Acid-Based Cationic Lipids as Effective Nonviral Gene Delivery Vectors

Biological and Pharmaceutical Bulletin 39(7), 1112-1120, 2016
NAID 130005160778

Non-ionic Surfactant Modified Cationic Liposomes Mediated Gene Transfection in Vitro and in the Mouse Lung(Biopharmacy)

Biological & pharmaceutical bulletin 32(2), 311-315, 2009-02-01
NAID 110007042144

In Vitro and In Vivo Tumor Suppressive Activity Induced by Human Telomerase Transcriptase-Targeting Antisense Oligonucleotides Mediated by Cationic Liposomes(CELL AND TISSUE ENGINEERING)