Cold Spring Harbor protocols.Cold Spring Harb Protoc.2010 Dec 1;2010(12):pdb.prot5535. doi: 10.1101/pdb.prot5535.
It is possible to microinject Xenopus oocytes that are still contained within their ovarian follicles, but most researchers find it more convenient to work with defolliculated oocytes. Defolliculation can be carried out enzymatically by treatment with collagenase, or it can be performed manually. En
Mitochondria play a vital role during oocyte maturation, fertilization, and embryo development. In this study, confocal microscopy with the mitochondrial membrane potential-sensitive dye JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide) was used to investigate mitoc
Native ion current coupled to purinergic activation via basal and mechanically induced ATP release in Xenopus follicles.
Saldaña C1, Garay E, Rangel GE, Reyes LM, Arellano RO.
Journal of cellular physiology.J Cell Physiol.2009 Feb;218(2):355-65. doi: 10.1002/jcp.21611.
Xenopus follicle-enclosed oocytes are endowed with purinergic receptors located in the follicular cell membrane; their stimulation by ATP elicits an electrical response that includes generation of a fast inward current (F(Cl)) carried by Cl(-). Here, it was found that mechanical stimulation of the f
Manual defolliculation of Xenopus oocytes uiowadblab Subscribe Subscribed Unsubscribe 5 5 Loading... Loading... Working... Add to Want to watch this again later? Sign in to add this video to a playlist. Sign in Share Sign in ...
INTRODUCTION It is possible to microinject Xenopus oocytes that are still contained within their ovarian follicles, but most researchers find it more convenient to work with defolliculated oocytes. Defolliculation can be ...