デアセトキシセファロス・リン

English Journal

Modified Deacetylcephalosporin C Synthase for the Biotransformation of Semisynthetic Cephalosporins.

Balakrishnan N1, Ganesan S1, Rajasekaran P1, Rajendran L1, Teddu S1, Durairaaj M2.
Applied and environmental microbiology.Appl Environ Microbiol.2016 Jun 13;82(13):3711-20. doi: 10.1128/AEM.00174-16. Print 2016 Jul 1.
Deacetylcephalosporin C synthase (DACS), a 2-oxoglutarate-dependent oxygenase synthesized by Streptomyces clavuligerus, transforms an inert methyl group of deacetoxycephalosporin C (DAOC) into an active hydroxyl group of deacetylcephalosporin C (DAC) during the biosynthesis of cephalosporin. It is a
PMID 27084018

[Evaluation of penicillin expandase mutants and complex substrate inhibition characteristics at high concentrations of penicillin G].

Wu L, Fan K, Ji J, Yang K.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology.Sheng Wu Gong Cheng Xue Bao.2015 Dec;31(12):1690-9.
Penicillin expandase, also known as deacetoxycephalosporin C synthase (DAOCS), is an essential enzyme involved in cephalosporin C biosynthesis. To evaluate the catalytic behaviors of penicillin expandase under high penicillin G concentration and to identify mutants suitable for industrial applicatio
PMID 27093832

Reconstitution of TCA cycle with DAOCS to engineer Escherichia coli into an efficient whole cell catalyst of penicillin G.

Lin B1, Fan K2, Zhao J1, Ji J2, Wu L2, Yang K3, Tao Y4.
Proceedings of the National Academy of Sciences of the United States of America.Proc Natl Acad Sci U S A.2015 Aug 11;112(32):9855-9. doi: 10.1073/pnas.1502866112. Epub 2015 Jul 27.
Many medically useful semisynthetic cephalosporins are derived from 7-aminodeacetoxycephalosporanic acid (7-ADCA), which has been traditionally made by the polluting chemical method. Here, a whole-cell biocatalytic process based on an engineered Escherichia coli strain expressing 2-oxoglutarate-depe
PMID 26216972

Japanese Journal

Effect of Solvents on Bioconversion of Penicillin G to Deacetoxycephalosporin G.

Mutational Evidence Supporting the Involvement of Tripartite Residues His183,Asp185,and His243 in Streptomyces clavuligerus Deacetoxycephalosporin C Synthase for Catalysis

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