English Journal
- [A method to determine the structure of the complex of enzyme and its substrate using 6-phosphate-beta-glucosidase at low temperature].
- Wang W, Liu Y, Li H.
- Sheng wu gong cheng xue bao = Chinese journal of biotechnology.Sheng Wu Gong Cheng Xue Bao.2013 Dec;29(12):1828-35.
- To capture a state of the enzyme in complex with an intact substrate, we developed and adopted a novel freezing method in crystal preparation procedure. Neither the elimination of the catalytically indispensable ligands, nor mutation or modification of the active site is required. At -20 degrees C,
- PMID 24660630
- Crystallographic cryoenzymology and the legacy of Tony Fink.
- Petsko GA1.
- Current protein & peptide science.Curr Protein Pept Sci.2009 Oct;10(5):416-23.
- During the past thirty years significant contributions to our understanding of the structural origins of the catalytic power of enzymes have come from solution and crystallographic studies of enzyme-substrate and enzyme-intermediate complexes trapped at subzero temperatures, a field that was pioneer
- PMID 19538155
- Three decades of the class A beta-lactamase acyl-enzyme.
- Fisher JF1, Mobashery S.
- Current protein & peptide science.Curr Protein Pept Sci.2009 Oct;10(5):401-7.
- The discovery that the mechanism of beta-lactam hydrolysis catalyzed by the class A (active site serine-dependent) beta-lactamases proceeds via an acyl-enzyme intermediate was made thirty years ago. Since this discovery, the active site circumstance that enables acylation of the active site serine a
- PMID 19538154
Japanese Journal
- 結晶構造から見た酵素の反応機構--セリンプロテア-ゼ系を題材として (結晶構造から化学反応へ<特集>)
- 広野 修一,三井 幸雄,飯高 洋一
- 日本結晶学会誌 24(1), 65-75, 1982
- … Finally, we introduce "X-ray cryoenzymology" or "low temperture protein crystallography", the apparently most effective technique for determining the details of the interaction of enzymes with their actual substrates, which will be a popular method in no distant future. …
- NAID 130000784298
- Cryoenzymology in aqueous media: micellar solubilized water clusters.
- DOUZOU P.
- Proc. Natl. Acad. Sci. USA 76, 681-684, 1979
- NAID 30016268054
Related Links
- 1. CRC Crit Rev Biochem. 1981;11(2):145-207. Cryoenzymology. Fink AL, Cartwright SJ. A detailed understanding of the mechanism of enzyme action requires a correspondingly detailed knowledge of the structures of the ...
- 1. Curr Protein Pept Sci. 2009 Oct;10(5):408-15. Cryoenzymology: enzyme action in slow motion. Dunn BM(1), Uversky VN. Author information: (1)Department of Biochemistry and Molecular Biology, University of Florida ...
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★リンクテーブル★
| リンク元 | 「cryoenzymological」「低温酵素学」 |
「cryoenzymological」
- 低温酵素学的な、低温酵素学の