Control of expression of the RNases J1 and J2 in Bacillus subtilis.
Jamalli A1, Hébert A, Zig L, Putzer H.
Journal of bacteriology.J Bacteriol.2014 Jan;196(2):318-24. doi: 10.1128/JB.01053-13. Epub 2013 Nov 1.
In Bacillus subtilis, the dual activity 5' exo- and endoribonucleases J1 and J2 are important players in mRNA and stable RNA maturation and degradation. Recent work has improved our understanding of their structure and mechanism of action and identified numerous RNA substrates. However, almost nothi
Analysis of transcription factor networks using IVV method.
Ohashi H1, Fujimori S, Hirai N, Yanagawa H, Miyamoto-Sato E.
Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2014;1164:15-22. doi: 10.1007/978-1-4939-0805-9_2.
We have developed a simple and totally in vitro selection procedure based on cell-free cotranslation using a highly stable and efficient in vitro virus (IVV). Cell-free cotranslation of tagged bait and prey proteins is advantageous for the formation of protein complexes and allows high-throughput an
Vectors bicistronically linking a gene of interest to the SV40 large T antigen in combination with the SV40 origin of replication enhance transient protein expression and luciferase reporter activity.
The simian virus 40 large T antigen (SVLT) induces replication of plasmids bearing the SV40 origin of replication (SV40 ori) within mammalian cells. The internal ribosomal entry site (IRES) is an element that allows for the cotranslation of proteins from one polycistronic mRNA. Through the combinati
cotranslation Source: Oxford Dictionary of Biochemistry and Molecular Biology Author(s): Richard CammackRichard Cammack, Teresa AtwoodTeresa Atwood, Peter CampbellPeter Campbell, Howard ParishHoward Parish, Anthony ...
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