Overexpression of miR-30a in lung adenocarcinoma A549 cell line inhibits migration and invasion via targeting EYA2.
Yuan Y1, Zheng S1, Li Q1, Xiang X2, Gao T1, Ran P1, Sun L1, Huang Q1, Xie F1, Du J1, Xiao C3.
Acta biochimica et biophysica Sinica.Acta Biochim Biophys Sin (Shanghai).2016 Mar;48(3):220-8. doi: 10.1093/abbs/gmv139. Epub 2016 Feb 1.
MicroRNAs (miRNAs) are a class of small non-coding RNAs and closely related to the pathogenesis of cancers. Increasing evidence indicates that miR-30a plays a profound role during the development of cancers. However, the functions of miR-30a in non-small-cell lung cancer (NSCLC) are still ambiguous.
Role of Phosphatidic Acid Phosphatase Domain Containing 2 in Squalestatin 1-Mediated Activation of the Constitutive Androstane Receptor in Primary Cultured Rat Hepatocytes.
Pant A1, Kocarek TA2.
Drug metabolism and disposition: the biological fate of chemicals.Drug Metab Dispos.2016 Mar;44(3):352-5. doi: 10.1124/dmd.115.068437. Epub 2015 Dec 23.
Farnesyl pyrophosphate (FPP) is a branch-point intermediate in the mevalonate pathway that is normally converted mainly to squalene by squalene synthase in the first committed step of sterol biosynthesis. Treatment with the squalene synthase inhibitor squalestatin 1 (SQ1) causes accumulation of FPP,
MicroRNA-503 promotes angiotensin II-induced cardiac fibrosis by targeting Apelin-13.
Zhou Y1, Deng L1, Zhao D1, Chen L1, Yao Z1, Guo X1, Liu X1, Lv L1, Leng B1, Xu W2, Qiao G1, Shan H1.
Journal of cellular and molecular medicine.J Cell Mol Med.2016 Mar;20(3):495-505. doi: 10.1111/jcmm.12754. Epub 2016 Jan 12.
Cardiac fibrosis is a major cause of heart failure. MicroRNAs (miRs) are important epigenetic regulators of cardiac function and cardiovascular diseases, including cardiac fibrosis. This study aimed to explore the role of miR-503 and its mechanisms in regulating cardiac fibrosis. miR-503 was found u
Cotransfection refers to the simultaneous transfection with two separate nucleic acid molecules, such as plasmid DNA and siRNA. Cotransfection is a common procedure for stable transfection. ... Lipofectamine® 3000 reagent ...
Transfection in multiwell plates — preparing a master mix If you are performing transfection in multiwell plates, prepare a master mix of transfection complexes or of transfection reagent and culture medium (depending on the protocol ...