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For other uses, see Confluence (disambiguation).
In cell culture biology, confluence is the term commonly used as an estimate of the number of adherent cells in a culture dish or a flask, referring to the proportion of the surface which is covered by cells. For example, 50 percent confluence means roughly half of the surface is covered and there is still room for cells to grow. 100 percent confluence means the surface is completely covered by the cells, and no more room is left for the cells to grow as a monolayer.
Many cell lines exhibit differences in growth rate or gene expression depending on the degree of confluence. Cells are typically passaged before becoming fully confluent in order to maintain their proliferation phenotype. Some cell types are not limited by contact inhibition, such as immortalized cells, may continue to divide and form layers on top of the parent cells. To achieve optimal and consistent results, experiments are usually performed using cells at a particular confluence, depending on the cell type.
Contents
- 1 Estimation
- 2 Hemocytometer
- 3 References
Estimation
Rule of thumb
Usually by comparing the amount of space covered by cells with the unoccupied spaces using naked eye, you will get an estimate of percent confluency.
Hemocytometer
The hemocytometer can be used to count cells.
References
English Journal
- High sensitivity plasmonic biosensor based on nanoimprinted quasi 3D nanosquares for cell detection.
- Zhu S1, Li H, Yang M, Pang SW.
- Nanotechnology.Nanotechnology.2016 Jul 22;27(29):295101. doi: 10.1088/0957-4484/27/29/295101. Epub 2016 Jun 8.
- Quasi three-dimensional (3D) plasmonic nanostructures consisting of Au nanosquares on top of SU-8 nanopillars and Au nanoholes on the bottom were developed and fabricated using nanoimprint lithography with simultaneous thermal and UV exposure. These 3D plasmonic nanostructures were used to detect ce
- PMID 27275952
- An expert protocol for immunofluorescent detection of calcium channels in tsA-201 cells.
- Koch P1, Herzig S2, Matthes J3.
- Journal of pharmacological and toxicological methods.J Pharmacol Toxicol Methods.2016 Jul 12;82:20-25. doi: 10.1016/j.vascn.2016.07.001. [Epub ahead of print]
- INTRODUCTION: Pore-forming subunits of voltage gated calcium channels (VGCC) are large membrane proteins (260kDa) containing 24 transmembrane domains. Despite transfection with viral promoter driven vectors, biochemical analysis of VGCC is often hampered by rather low expression levels in heterologo
- PMID 27421665
- Deletion of angiotensin II type 2 receptor accelerates adipogenesis in murine mesenchymal stem cells via Wnt10b/beta-catenin signaling.
- Matsushita K1,2, Wu Y1, Pratt RE1, Dzau VJ1,3.
- Laboratory investigation; a journal of technical methods and pathology.Lab Invest.2016 Jun 13. doi: 10.1038/labinvest.2016.66. [Epub ahead of print]
- Recent evidence suggests that the renin-angiotensin system (RAS) has a vital role in adipocyte biology and the pathophysiology of metabolic syndrome. Obesity is the main culprit of metabolic syndrome; and mesenchymal stem cells (MSCs) have been forwarded as a major source of adipocyte generation. Pr
- PMID 27295344
Japanese Journal
- 初級および初中級英語学習者へのレスポンス : トークン実習の前と後
- J028032 肝星細胞の遊走におけるせん断応力の影響
- 年次大会 : Mechanical Engineering Congress, Japan 2012, "J028032-1"-"J028032-3", 2012-09-09
- NAID 110009994434
- 流暢さの再検討 : 話し手と聞き手の共同作業を通して
Related Links
- confluency 【名】〔細胞の〕密集度 - アルクがお届けする進化するオンライン英和・和英辞書データベース。一般的な単語や連語から、イディオム、専門用語、スラングまで幅広く収録。
- Confluency Solutions Provides website design and hosting for insurance agents. Other services include search engine and social media marketing. ... Insurance Agency Websites Confluency's insurance agency websites are search ...
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