融合体、共組換え体

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2014/07/06 10:34:08」(JST)

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English Journal

A molecular survey of the dissemination of two blaKPC-harboring IncFIA plasmids in New Jersey and New York hospitals.

Chen L, Chavda KD, Melano RG, Hong T, Rojtman AD, Jacobs MR, Bonomo RA, Kreiswirth BN.Author information Public Health Research Institute Center, New Jersey Medical School, Rutgers University, Newark, New Jersey 07103.AbstractKlebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae have spread worldwide and become a major threat in health care facilities. Transmission of blaKPC,the plasmid-borne KPC gene, can be mediated by both clonal spread and horizontal transfer. Here we report the complete nucleotide sequences of two novel blaKPC-3-harboring IncFIA plasmids, pBK30661 and pBK30683. pBK30661 is 74 kb in length with a mosaic plasmid structure, sharing homologies to several other plasmids, but lacks the plasmid transfer operon (tra) and origin of transfer (oriT) that are required for plasmid transfer. pBK30683 is a conjugative plasmid with a cointegrate plasmid structure, comprising a 72 kb element that highly resembles pBK30661 (> 99.9% nucleotide identities) along with an extra 68 kb element that harbors tra and oriT. A PCR scheme was designed to detect the distribution of blaKPC-harboring IncFIA (pBK30661 and pBK30683-like) plasmids from a collection of clinical Enterobacteriaceae isolates from ten New Jersey and New York hospitals. KPC-harboring IncFIA plasmids were found in 20% of 491 K. pneumoniae isolates and all carried blaKPC-3. pBK30661-like plasmids were mainly identified in the epidemic ST258 K. pneumoniae clone, while pBK30683-like plasmids were widely distributed in ST258 and other K. pneumoniae sequence types, as well as among other non-K. pneumoniae Enterobacteriaceae species. This suggests that both clonal spread and horizontal plasmid transfer contributed to the dissemination of blaKPC-harboring IncFIA plasmids in our area. Further studies are needed to understand the distribution of this plasmid group in other health care regions and decipher the origin of pBK30661 and pBK30683-like plasmids.
Antimicrobial agents and chemotherapy.Antimicrob Agents Chemother.2014 Feb 3. [Epub ahead of print]
Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae have spread worldwide and become a major threat in health care facilities. Transmission of blaKPC,the plasmid-borne KPC gene, can be mediated by both clonal spread and horizontal transfer. Here we report the complete nucleotide sequen
PMID 24492370

Complete sequence analysis of two cryptic plasmids from Bifidobacterium kashiwanohense JCM 15439 (type strain) isolated from healthy infant feces.

Takahata M, Toh H, Nakano A, Takagi M, Murakami M, Ishii Y, Takizawa T, Tanabe S, Morita H.Author information School of Veterinary Medicine, Azabu University, Sagamihara; BioBank Co., Ltd, Okayama.AbstractBifidobacterial plasmids reported so far are derived from a limited number of strains and plasmids of bifidobacterial type strains isolated from humans are unknown. We found that Bifidobacterium kashiwanohense JCM 15439 (type strain) isolated from a healthy infant contained two cryptic plasmids, designated pBBKW-1 and pBBKW-2. We determined and analyzed the complete sequences of both plasmids. pBBKW-1 (7716 bp) was predicted to replicate by a rolling-circle mechanism and encode six protein-coding genes, two of which are putative replication proteins. pBBKW-1 seems to be a cointegrate plasmid containing two copies of the plasmid pMG1 from Bifidobacterium longum. pBBKW-2 (2920 bp) was predicted to encode six protein-coding genes and be a theta-type replicating plasmid, which has been reported to be more stable than a rolling circle-type replicating plasmid frequently found in bifidobacteria. Our finding will provide new insights into safe recombinant plasmid constructions for humans.
Animal science journal = Nihon chikusan Gakkaihō.Anim Sci J.2014 Feb;85(2):158-63. doi: 10.1111/asj.12095. Epub 2013 Jul 19.
Bifidobacterial plasmids reported so far are derived from a limited number of strains and plasmids of bifidobacterial type strains isolated from humans are unknown. We found that Bifidobacterium kashiwanohense JCM 15439 (type strain) isolated from a healthy infant contained two cryptic plasmids, d
PMID 23865717

The resolution and regeneration of a cointegrate plasmid reveals a model for plasmid evolution mediated by conjugation and oriT site-specific recombination.

Wang P, Zhang C, Zhu Y, Deng Y, Guo S, Peng D, Ruan L, Sun M.Author information State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.AbstractCointegrate plasmids are useful models for the study of plasmid evolution if their evolutionary processes can be replicated under laboratory conditions. pBMB0228, a 17 706 bp native plasmid originally isolated from Bacillus thuringiensis strain YBT-1518, carries two nematicidal crystal protein genes, cry6Aa and cry55Aa. In this study, we show that pBMB0228 is in fact a cointegrate of two plasmids and contains two functional replication regions and two functional mobilization regions. Upon introduction into B. thuringiensis strain BMB171, pBMB0228 spontaneously resolves into two constituent plasmids via recombination at its oriT1 and oriT2 sites. The resolution does not require conjugation but can be promoted by conjugation. We further confirm that the resolution is mediated by oriT site-specific recombination requiring Mob02281 or Mob02282. Additionally, the two constituent plasmids of pBMB0228 are mobilizable, and can fuse back via oriT site-specific integration after entering into the same cell by conjugation. Our study confirms that native plasmid can reversibly interconvert between a cointegrate structure and its constituent plasmids. This study provides insight into the evolution of cointegrate plasmids, linking plasmid evolution with conjugation and the oriT site-specific recombination function of relaxase.
Environmental microbiology.Environ Microbiol.2013 Dec;15(12):3305-18. doi: 10.1111/1462-2920.12177. Epub 2013 Jul 4.
Cointegrate plasmids are useful models for the study of plasmid evolution if their evolutionary processes can be replicated under laboratory conditions. pBMB0228, a 17 706 bp native plasmid originally isolated from Bacillus thuringiensis strain YBT-1518, carries two nematicidal crystal protein g
PMID 23826996

Japanese Journal

IS3の転移による融合体形成

槌田謙,劉正風,関根靖彦,大坪栄一
日本分子生物学会年会プログラム・講演要旨集 21, 369, 1998-12-01
NAID 10002918560

A Rapid Method for Disrupting Genes in the Escherichia coli Genome

Kato Chinatsu,Ohmiya Ryusuke,Mizuno Takeshi
Bioscience, biotechnology, and biochemistry 62(9), 1826-1829, 1998-09-23
… It was found that the vector allows us to rapidly construct a disruption mutant for any gene on the chromosome as a cointegrate, furthermore, resolution of the resulting cointegrate can be surely accomplished by using a pair of the bla (ampicillin resistant) genes on the vector as a positive-selection marker. …
NAID 110002679331

Transduction of a Plasmid with an Inserted R4 Phage DNA Fragment in Streptomyces lividans

MORINO Tomio,TAKAHASHI Hideo
Bioscience, biotechnology, and biochemistry 61(6), 1047-1048, 1997-06-23
… A possible transduction mechanism through plasmid-phage cointegrate formation in vivo is discussed. …
NAID 110002678643