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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2014/02/04 11:15:44」(JST)
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- A tetracycline expression system in combination with Sox9 for cartilage tissue engineering.
- Yao Y1, He Y1, Guan Q1, Wu Q2.Author information 1MOE Key Lab. Bioinformatics, Center for Epigentics and Chromatin, School of Life Sciences, Tsinghua University, Beijing 100084, China.2MOE Key Lab. Bioinformatics, Center for Epigentics and Chromatin, School of Life Sciences, Tsinghua University, Beijing 100084, China. Electronic address: wuqiong@mail.tsinghua.edu.cn.AbstractCartilage tissue engineering using controllable transcriptional therapy together with synthetic biopolymer scaffolds shows higher potential for overcoming chondrocyte degradation and constructing artificial cartilages both in vivo and in vitro. Here, the potential regulating tetracycline expression (Tet-on) system was used to express Sox9 both in vivo and in vitro. Chondrocyte degradation was measured in vitro and overcome by Soxf9 expression. Experiments confirmed the feasibility of the combined use of Sox9 and Tet-on system in cartilage tissue engineering. Engineered poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) scaffolds were seeded with recombinant chondrocytes which were transfected with Tet-induced Sox9 expression; the scaffolds were implanted under the skin of 8-week-old rats. The experimental group was injected with Dox in the abdomen, while the control group was injected with normal saline. After 4 or 8 days of implantation in vivo, the newly formed pieces of articular chondrocytes were taken out and measured. Dox injection in vivo showed positive effect on recombinant chondrocytes, in which Sox9 expression was up-regulated by an inducible system with specific matrix proteins. The results demonstrate this controllable transcriptional therapy is a potential approach for tissue engineering.
- Biomaterials.Biomaterials.2014 Feb;35(6):1898-906. doi: 10.1016/j.biomaterials.2013.11.043. Epub 2013 Dec 8.
- Cartilage tissue engineering using controllable transcriptional therapy together with synthetic biopolymer scaffolds shows higher potential for overcoming chondrocyte degradation and constructing artificial cartilages both in vivo and in vitro. Here, the potential regulating tetracycline expressio
- PMID 24321708
- Chitosan-plasmid DNA nanoparticles encoding small hairpin RNA targeting MMP-3 and -13 to inhibit the expression of dedifferentiation related genes in expanded chondrocytes.
- Zhao J, Fan X, Zhang Q, Sun F, Li X, Xiong C, Zhang C, Fan H.Author information Department of Sports Medicine, Xi-Jing Hospital, the Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China; Department of Orthopaedic Surgery, the 82nd Hospital of PLA, Huai'an 223001, Jiangsu Province, China.AbstractOverexpression of matrix metalloproteinase (MMP)-3 and -13 can lead to the dedifferentiation of expanded chondrocytes. After implanting dedifferentiated cells for cartilage defect repair, graft failure may occur. Short hairpin RNA (shRNA) is a powerful genetic tool to reduce the expression of target genes. This study investigated the effects of chitosan-plasmid DNA (pDNA) nanoparticles encoding shRNA targeting MMP-3 and -13 on the dedifferentiation of expanded chondrocytes. The objective was to optimize the parameters of chitosan-pDNA formulation for achieving higher efficiency of pDNA delivery and gene silencing. The chitosan-pDNA nanoparticles were prepared using a complex coacervation process. Then the characteristics including size, shape, stability, and transfection efficiency were compared in different groups. The results indicated that chitosan of 800 kDa at N/P ratio of 4 and pH 7.0 was optimal to prepare chitosan-pDNA nanoparticles. These nanoparticles showed high DNA loading efficiency (95.8 ± 1.5%) and high gene transfection efficiency (24.5 ± 1.6%). After the expanded chondrocytes were transfected by chitosan-pDNA nanoparticles, MMP-3-610 and MMP-13-2024 groups showed greater suppression in mRNA and protein levels. The results indicated that chitosan-pDNA nanoparticles encoding shRNA targeting MMP-3 and -13 had great potential in silencing the dedifferentiation-related genes for regenerating prolonged and endurable cartilage. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 373-380, 2014.
- Journal of biomedical materials research. Part A.J Biomed Mater Res A.2014 Feb;102(2):373-80. doi: 10.1002/jbm.a.34711. Epub 2013 May 14.
- Overexpression of matrix metalloproteinase (MMP)-3 and -13 can lead to the dedifferentiation of expanded chondrocytes. After implanting dedifferentiated cells for cartilage defect repair, graft failure may occur. Short hairpin RNA (shRNA) is a powerful genetic tool to reduce the expression of target
- PMID 23520014
- Induction of mesenchymal stem cell chondrogenesis through sequential administration of growth factors within specific temporal windows.
- Handorf AM, Li WJ.Author information Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, Madison, Wisconsin; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin.AbstractHuman mesenchymal stem cells (hMSCs) are capable of differentiating into chondrocyte-like cells but fail to produce the quality or quantity of cartilage matrix compared to articular chondrocytes using current differentiation protocols. In this study, we aim to improve the chondrogenic differentiation of hMSCs through the sequential administration of multiple growth factors (GFs). We began by looking at differentiating hMSCs' cell surface GF receptor expression every 3 days throughout differentiation using flow cytometry and found that not only was receptor expression dynamic throughout differentiation, but ligand sensitivity was positively correlated with receptor expression, suggesting that differentiating hMSCs may have varying GF requirements depending on their stage of differentiation. We then constructed GF sequences by administering several prochondrogenic GFs singly every 3 days throughout differentiation and assaying the expression of a variety of cartilage-related genes using qPCR. The resulting chondrocytic phenotype of sequentially induced hMSCs was then compared to that of hMSCs induced under standard culture conditions using qPCR, dimethylmethylene blue assay, and histology. We found that while the initial GF sequence was unable to improve hMSC chondrogenesis, withdrawal of GF treatment at Day 9 of differentiation in pellet culture vastly improved the success of differentiation beyond that induced by TGFβ1 alone. Additional modifications allowed us to further improve chondrogenesis to levels comparable to that obtained by co-administration of TGFβ1 and BMP7 throughout differentiation. Taken together, we demonstrated the ability to improve the chondrocytic phenotype of differentiated hMSCs through the sequential administration of multiple GFs.
- Journal of cellular physiology.J Cell Physiol.2014 Feb;229(2):162-71. doi: 10.1002/jcp.24428.
- Human mesenchymal stem cells (hMSCs) are capable of differentiating into chondrocyte-like cells but fail to produce the quality or quantity of cartilage matrix compared to articular chondrocytes using current differentiation protocols. In this study, we aim to improve the chondrogenic differentiatio
- PMID 23996894
Japanese Journal
- Hyaluronan-based Biomaterials in Tissue Engineering(Prof. K Watanabe Memorial Article) :
- Acta histochemica et cytochemica 37(1), 1-5, 2004
- NAID 110003144310
- Dental Pulp Cells with Multi-Potential for Differentiation to Odontoblast and Chondroblast
- Journal of hard tissue biology 12(2), 49-55, 2003-12
- NAID 110001142070
- Dental Pulp Cells with Multi-Potential for Differentiation to Odontoblast and Chondroblast
- Journal of Hard Tissue Biology 12(2), 49-55, 2003
- NAID 130004480419
Related Links
- 軟骨芽細胞(英語: chondroblast)とは間充織幹細胞(en:mesenchymal stem cell )に 由来し、軟骨細胞(en:chondrocyte)に分化する細胞。軟骨芽細胞は軟骨細胞と 呼ばれる基質に組み込まれている。軟骨芽細胞は複数で空隙に位置するあるいは脱落 している ...
- A chondroblast is a cell which originates from a mesenchymal stem cell and forms chondrocytes, commonly known as cartilage cells. Chondroblasts that become embedded in the matrix are called chondrocytes. They lie in the space or ...
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★リンクテーブル★
| リンク元 | 「chondroblastic」「軟骨芽細胞」 |
「chondroblastic」
- 軟骨芽細胞の、軟骨母細胞性の