WordNet
- an animal sterol that is normally synthesized by the liver; the most abundant steroid in animal tissues (同)cholesterin
- any of the enzymes that catalyze biological oxidation
PrepTutorEJDIC
- コレステロール(胆汁・血液・神経組織などの脂肪性成分)
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2015/11/20 21:44:14」(JST)
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cholesterol oxidase |
Identifiers |
EC number |
1.1.3.6 |
CAS number |
9028-76-6 |
Databases |
IntEnz |
IntEnz view |
BRENDA |
BRENDA entry |
ExPASy |
NiceZyme view |
KEGG |
KEGG entry |
MetaCyc |
metabolic pathway |
PRIAM |
profile |
PDB structures |
RCSB PDB PDBe PDBsum |
Gene Ontology |
AmiGO / EGO |
Search |
PMC |
articles |
PubMed |
articles |
NCBI |
proteins |
|
Cholesterol oxidase substrate-binding domain |
crystal structure of cholesterol oxidase from b.sterolicum
|
Identifiers |
Symbol |
Chol_subst-bind |
Pfam |
PF09129 |
Pfam clan |
CL0277 |
InterPro |
IPR015213 |
SCOP |
1i19 |
SUPERFAMILY |
1i19 |
Available protein structures: |
Pfam |
structures |
PDB |
RCSB PDB; PDBe; PDBj |
PDBsum |
structure summary |
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In enzymology, a cholesterol oxidase (EC 1.1.3.6) is an enzyme that catalyzes the chemical reaction
- cholesterol + O2 cholest-4-en-3-one + H2O2
Thus, the two substrates of this enzyme are cholesterol and O2, whereas its two products are cholest-4-en-3-one and H2O2.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is cholesterol:oxygen oxidoreductase. Other names in common use include cholesterol- O2 oxidoreductase, 3beta-hydroxy steroid oxidoreductase, and 3beta-hydroxysteroid:oxygen oxidoreductase. This enzyme participates in bile acid biosynthesis.
The substrate-binding domain found in some bacterial cholesterol oxidases is composed of an eight-stranded mixed beta-pleated sheet and six alpha-helices. This domain is positioned over the isoalloxazine ring system of the FAD cofactor bound by the FAD-binding domain and forms the roof of the active site cavity, allowing for catalysis of oxidation and isomerisation of cholesterol to cholest-4-en-3-one.[1]
Structural studies
As of late 2007, 14 structures have been solved for this class of enzymes, with PDB accession codes 1B4V, 1B8S, 1CBO, 1CC2, 1COY, 1I19, 1IJH, 1MXT, 1N1P, 1N4U, 1N4V, 1N4W, 2GEW, and 3COX.
References
- ^ Coulombe R, Yue KQ, Ghisla S, Vrielink A (August 2001). "Oxygen access to the active site of cholesterol oxidase through a narrow channel is gated by an Arg-Glu pair". J. Biol. Chem. 276 (32): 30435–41. doi:10.1074/jbc.M104103200. PMID 11397813.
Further reading
- Richmond W (1973). "Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum". Clin. Chem. 19 (12): 1350–6. PMID 4757363.
- STADTMAN TC, CHERKES A, ANFINSEN CB (1954). "Studies on the microbiological degradation of cholesterol". J. Biol. Chem. 206 (2): 511–23. PMID 13143010.
This article incorporates text from the public domain Pfam and InterPro IPR015213
UpToDate Contents
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English Journal
- Biosensor based on glucose oxidase-nanoporous gold co-catalysis for glucose detection.
- Wu C1, Sun H1, Li Y1, Liu X1, Du X1, Wang X2, Xu P1.
- Biosensors & bioelectronics.Biosens Bioelectron.2015 Apr 15;66:350-5. doi: 10.1016/j.bios.2014.11.037. Epub 2014 Nov 24.
- Promoting the electrocatalytic oxidation of glucose is crucial in glucose biosensor design. In this study, nanoporous gold (NPG) was selected for glucose oxidase (GOx) immobilization and glucose biosensor fabrication because of its open, highly conductive, biocompatible, and interconnected porous st
- PMID 25463642
- Vascular injury in diabetic db/db mice is ameliorated by atorvastatin: role of Rac1/2-sensitive Nox-dependent pathways.
- Bruder-Nascimento T1, Callera GE2, Montezano AC3, He Y2, Antunes TT2, Cat AN3, Tostes RC1, Touyz RM2.
- Clinical science (London, England : 1979).Clin Sci (Lond).2015 Apr;128(7):411-23. doi: 10.1042/CS20140456.
- Oxidative stress [increased bioavailability of reactive oxygen species (ROS)] plays a role in the endothelial dysfunction and vascular inflammation, which underlie vascular damage in diabetes. Statins are cholesterol-lowering drugs that are vasoprotective in diabetes through unknown mechanisms. We t
- PMID 25358739
- Complementary probes reveal that phosphatidylserine is required for the proper transbilayer distribution of cholesterol.
- Maekawa M, Fairn GD.
- Journal of cell science.J Cell Sci.2015 Feb 6. pii: jcs.164715. [Epub ahead of print]
- Cholesterol is an essential component of metazoan cellular membranes and helps to maintain the structural integrity and fluidity of the plasma membrane. Here, we developed a cholesterol biosensor, termed D4H, based on the fourth domain of Clostridium perfringens theta-toxin, which recognizes cholest
- PMID 25663704
Japanese Journal
- Cholesterol oxidase with high catalytic activity from Pseudomonas aeruginosa : Screening, molecular genetic analysis, expression and characterization
- Journal of bioscience and bioengineering 120(1), 24-30, 2015-07
- NAID 40020537941
- Cholesterol oxidase with high catalytic activity from Pseudomonas aeruginosa : Screening, molecular genetic analysis, expression and characterization(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- ミセル電解法で作成したフラビン酸素電極の直接電子移動
Related Links
- COO-321 TOYOBO ENZYMES (Diagnostic Reagent Grade) CHOLESTEROL OXIDASE from Microorganism Cholesterol:oxygen oxidoreductase(EC 1.1.3.6) HO +O 2 Cholesterol O +H 2 O 2 Cholest-4-en-3-one PREPARATION ...
- 1. OBJECTIVE To standardize a procedure for the determination of the enzymatic assay of Cholesterol Oxidase. 2. SCOPE This procedure applies to products that have a specification for the enzymatic activity of cholesterol oxidase.
★リンクテーブル★
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- 英
- cholesterol oxidase
- 関
- コレステロールオキシダーゼ
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- 英
- cholesterol oxidase
- 関
- コレステロール酸化酵素
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コレステロール