Conventional methods for screening promoters in anaerobic bacteria are generally based on detection of enzymatic reactions and thus usually complicated or strain specific. Therefore a more efficient and universal method will be valuable. Here, using cellulolytic bacteria Clostridium cellulolyticum H
The Journal of antimicrobial chemotherapy.J Antimicrob Chemother.2015 Sep;70(9):2576-88. doi: 10.1093/jac/dkv161. Epub 2015 Jul 14.
OBJECTIVES: The spread of MDR bacteria represents a serious threat to human society and novel antibiotic drugs, preferably from new chemical classes, are urgently needed. Closthioamide was isolated from the strictly anaerobic bacterium Clostridium cellulolyticum and belongs to a new class of natural
Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase.
Xu T1, Li Y1, Shi Z1, Hemme CL1, Li Y1, Zhu Y2, Van Nostrand JD1, He Z1, Zhou J3.
Applied and environmental microbiology.Appl Environ Microbiol.2015 Jul;81(13):4423-31. doi: 10.1128/AEM.00873-15. Epub 2015 Apr 24.
The CRISPR-Cas9 system is a powerful and revolutionary genome-editing tool for eukaryotic genomes, but its use in bacterial genomes is very limited. Here, we investigated the use of the Streptococcus pyogenes CRISPR-Cas9 system in editing the genome of Clostridium cellulolyticum, a model microorgani