cRNAクローニング
WordNet
- a general term for the research activity that creates a copy of some biological entity (a gene or organism or cell)
UpToDate Contents
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English Journal
- Cloning and characterization of the ionotropic GABA receptor subunit ρ1 from pig (Sus scrofa).
- Reyes-Ruiz JM1, Limon A2, Miledi R2.
- Neuroscience letters.Neurosci Lett.2014 Jan 13;558:78-81. doi: 10.1016/j.neulet.2013.11.003. Epub 2013 Nov 14.
- Since human and pig eyes have remarkably anatomical and physiological similitudes swine models have been broadly used for functional studies and therapeutic research. Recently, a GABAρ-mediated relaxation of retinal vascularity suggested that GABAρ signaling may be used to improve retinal blood fl
- PMID 24239647
- Optimizing plant transporter expression in Xenopus oocytes.
- Feng H, Xia X, Fan X1, Xu G, Miller AJ.
- Plant methods.Plant Methods.2013 Dec 20;9(1):48. doi: 10.1186/1746-4811-9-48.
- BACKGROUND: Rapid improvements in DNA synthesis technology are revolutionizing gene cloning and the characterization of their encoded proteins. Xenopus laevis oocytes are a commonly used heterologous system for the expression and functional characterization of membrane proteins. For many plant prote
- PMID 24359672
- Generation and validation of a zebrafish model of EAST (epilepsy, ataxia, sensorineural deafness and tubulopathy) syndrome.
- Mahmood F1, Mozere M, Zdebik AA, Stanescu HC, Tobin J, Beales PL, Kleta R, Bockenhauer D, Russell C.
- Disease models & mechanisms.Dis Model Mech.2013 May;6(3):652-60. doi: 10.1242/dmm.009480. Epub 2013 Feb 14.
- Recessive mutations in KCNJ10, which encodes an inwardly rectifying potassium channel, were recently identified as the cause of EAST syndrome, a severe and disabling multi-organ disorder consisting of epilepsy, ataxia, sensorineural deafness and tubulopathy that becomes clinically apparent with seiz
- PMID 23471908
Japanese Journal
- Molecular characterization and histochemical demonstration of salmon olfactory marker protein in the olfactory epithelium of lacustrine sockeye salmon (Oncorhynchus nerka)
- Kudo H.,Doi Y.,Ueda H.,Kaeriyama M.
- Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology 154(1), 142-150, 2009-09
- … By in situ hybridization using a digoxigeninlabeled salmon OMP cRNA probe, signals for salmon OMP mRNA were observed preferentially in the perinuclear regions of the ORNs. … The present study is the first to describe cDNA cloning of OMP in salmon olfactory epithelium, and indicate that OMP is a useful molecular marker for the detection of the ORNs in Pacific salmon. …
- NAID 120001425535
- Cloning and functional characterization of a novel up-regulator, cartregulin, of carnitine transporter, OCTN2
- Nagai Kazuhiko,Takikawa Osamu,Kawakami Naoko,Fukao Mitsuhiro,Soma Takamitsu,Oda Atsushi,Nishiya Tadashi,Hayashi Misato,Lu Lingyun,Nakano Manabu,Kajita Emi,Fujita Hiroyoshi,Miwa Soichi
- Archives of Biochemistry and Biophysics 452(1), 29-37, 2006-08-01
- … When OCTN2 was expressed in Xenopus oocytes by injection of its cRNA, its transport activity was enhanced by co-expression of the novel protein. …
- NAID 120000960814
- An mRNA amplification procedure with directional cDNA cloning and strand-specific cRNA synthesis for comprehensive gene expression analysis
Related Links
- Preparation of the cDNA library by unidirectional cloning of a single copy of amplified cDNA Construction of a unique restriction end on each of both cDNA termini would enable unidirectional cloning of a single copy of cDNA. Treatment ...
- Abstract The synthesis of cRNA probes for in situ hybridization is usually accomplished with transcription systems using cloning vectors that contain bacteriophage RNA polymerase promoters. In this report we ...
- Highlights • Poly(A) addition and SMART technology were employed for RACE cDNA cloning of influenza A virus noncoding regions. • Specific influenza A virus primers were used for cloning NCRs from vRNA, cRNA and capped ...
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- 関
- complementary RNA、cRNA probe