ブロムクレゾールパープル、ブロモクレゾールパープル
WordNet
- of a color intermediate between red and blue (同)violet, purplish
- color purple (同)empurple, purpurate
- become purple
- of imperial status; "he was born to the purple"
- a purple color or pigment (同)purpleness
PrepTutorEJDIC
- 〈U〉『紫色』 / 〈U〉(特に,昔の王家や高位の人がつけた)紫布,紫衣 / 《the ~》王位,高位 / 『紫色の』
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/07/11 11:07:29」(JST)
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Bromocresol purple
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| Names |
| IUPAC name
4,4'-(1,1-Dioxido-3H-2,1-benzoxathiole-3,3-diyl)-bis(2-bromo-6-methylphenol)
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| Other names
5′,5″-Dibromo-o-cresolsulfonephthalein
Bromcresol purple
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| Identifiers |
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CAS Number
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115-40-2 Y |
| ChEBI |
CHEBI:86154 N |
| ChemSpider |
7974 Y |
| Jmol 3D model |
Interactive image |
| PubChem |
8273 |
InChI
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InChI=1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3 Y
Key: ABIUHPWEYMSGSR-UHFFFAOYSA-N Y
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InChI=1/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3
Key: ABIUHPWEYMSGSR-UHFFFAOYAH
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InChI=1/C21H17BrO5S/c1-12-9-14(7-8-18(12)23)21(15-10-13(2)20(24)17(22)11-15)16-5-3-4-6-19(16)28(25,26)27-21/h3-11,23-24H,1-2H3
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SMILES
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Brc1c(O)c(cc(c1)C3(OS(=O)(=O)c2ccccc23)c4cc(c(O)c(Br)c4)C)C
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| Properties |
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Chemical formula
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C21H16Br2O5S |
| Molar mass |
540.22 g·mol−1 |
| Appearance |
Purple powder |
| Melting point |
241 to 242 °C (466 to 468 °F; 514 to 515 K) (decomposition) |
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Solubility in water
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< 0.1 % |
| Hazards |
| R-phrases |
R36/37/38 |
| S-phrases |
S26, S36 |
| NFPA 704 |
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Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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| N verify (what is YN ?) |
| Infobox references |
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Bromocresol purple (BCP) or 5′,5″-dibromo-o-cresolsulfophthalein, is a dye of the triphenylmethane family (triarylmethane dyes) and a pH indicator. It is colored yellow below pH 5.2, and violet above pH 6.8. In its cyclic sulfonate ester form, it has a pKa value of 6.3, and is usually prepared as a 0.04% aqueous solution.[1]
Contents
- 1 Uses
- 2 References
- 3 External links
Uses
A sample of bromocresol purple in its violet form
| Bromocresol purple (pH indicator) |
| below pH 5.2 |
|
above pH 6.8 |
| 5.2 |
⇌ |
6.8 |
Bromocresol purple is used in medical laboratories to measure albumin, although it is no better in this role than the older method using bromocresol green.[2] In microbiology, it is used for staining dead cells based on their acidity, and for the isolation and assaying of lactic acid bacteria.[3][4]
In photographic processing, it can be used as an additive to acid stop baths to indicate that the bath has reached neutral pH and needs to be replaced.[5]
Bromocresol purple milk solids glucose agar is used as a medium used to distinguish dermatophytes from bacteria and other organisms in cases of ringworm fungus (T. verrucosum) infestation in cattle and other animals.[6][7]
pH Indicator
Similar to bromocresol green, the structure of bromocresol purple changes with pH. The low pH (acidic) form is yellow in solution and the high pH (basic) form is purple; the sultone (cyclic sulfonic ester) is the acidic form in the following equilibrium.
References
- ^ "Bromocresol Purple". NCBI PubChem. National Center for Biotechnology Information.
- ^ McGinlay, J.M.; Payne, R.B. (July 1988). "Serum albumin by dye-binding: bromocresol green or bromocresol purple? The case for conservatism." (PDF). Annals of Clinical Biochemistry 25 (4): 417–421. doi:10.1177/000456328802500417. PMID 3214125.
- ^ Kurzweilová, H.; Sigler, K. (November 1993). "Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity.". Yeast 9 (11): 1207–1211. doi:10.1002/yea.320091107. PMID 7509098.
- ^ Lee, H.M.; Lee, Y. (June 2008). "A differential medium for lactic acid-producing bacteria in a mixed culture". Letters of Applied Microbiology 46 (6): 676–681. doi:10.1111/j.1472-765X.2008.02371.x. PMID 18444977.
- ^ Anchell, Steve (2016). The Darkroom Cookbook (4 ed.). Routledge. ISBN 9781317337607 – via Google Books.
- ^ Kane, J.; Summerbell, R.; Sigler, L.; Krajden, S.; Land, G. (1997). Laboratory Handbook of Dermatophytes: A Clinical Guide and Laboratory Handbook of Dermatophytes and Other Filamentous Fungi from Skin, Hair, and Nails. Belmont, CA: Star Publishing Company. ISBN 9780898631579.
- ^ Beneke, E. S.; Rogers, A. L. (1996). Medical Mycology and Human Mycoses (illustrated ed.). Belmont, CA: Star Publishing Compnay. pp. 85–90. ISBN 9780898631753.
External links
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Wikimedia Commons has media related to Bromocresol purple. |
- Material Safety Data Sheet from Oxford University
UpToDate Contents
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English Journal
- Use of two sulfonthalein dyes in the extraction-free spectrophotometric assay of tramadol in dosage forms and in spiked human urine based on ion-pair reaction.
- Vinay KB, Revannasiddappa HD, Rajendraprasad N, Ramesh PJ, Xavier CM, Basavaiah K.SourceDepartment of Chemistry, Manasagangothri, University of Mysore, Mysore 570 006, Karnataka, India.
- Drug testing and analysis.Drug Test Anal.2012 Feb;4(2):116-22. doi: 10.1002/dta.247. Epub 2011 Feb 3.
- Tramadol is a centrally acting analgesic used in the prevention and treatment of moderate to severe pain. Two sensitive, selective, and rapid spectrophotometric methods are described for the determination of tramadol in its dosage forms and in spiked human urine. The methods are based on formation o
- PMID 21290614
- Influence of the assay for measuring serum albumin on corrected total calcium in chronic hemodialysis patients.
- Kato A, Takita T, Furuhashi M, Fujimoto T, Suzuki H, Hakamada M, Maruyama Y.SourceBlood Purification Unit, Hamamatsu University School of Medicine Department of Nephrology, Maruyama Hospital, 1-20-1 Handayama, Higashi-ku, Hamamatsu-shi, Shizuoka, Japan. a.kato@hama-med.ac.jp
- Therapeutic apheresis and dialysis : official peer-reviewed journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy.Ther Apher Dial.2011 Dec;15(6):540-6. doi: 10.1111/j.1744-9987.2011.00997.x.
- The Japanese Society for Dialysis Therapy guideline for secondary hyperparathyroidism recommends the use of albumin-corrected serum Ca as a therapeutic target in chronic hemodialysis patients; however, the assay used for albumin measurement may affect the corrected Ca level. In this study, we examin
- PMID 22107690
Japanese Journal
- 当院におけるアルブミン測定法と高張アルブミン製剤濃度の変更による高張アルブミン製剤の使用状況への影響と適正使用推進方策の検討
- 血清アルブミン値を計算に用いる臨床的栄養指標の問題点:測定法改良による指標値の乖離
- 大学病院におけるアルブミン製剤の適正使用推進の効果と問題点
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