ビオチン化デキストランアミン
- 関
- BDA
WordNet
- a compound derived from ammonia by replacing hydrogen atoms by univalent hydrocarbon radicals (同)aminoalkane
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2012/12/03 15:12:48」(JST)
[Wiki en表示]
Biotinylated dextran amines (BDA) are organic compounds used as anterograde and retrograde neuroanatomical tracers. They can be used for labeling the source as well as the point of termination of neural connections and therefore to study neural pathways.
BDA is delivered into the nervous system by iontophoretic or pressure injection and visualized with an avidin-biotinylated Horseradish peroxidase procedure, followed by a standard or metal-enhanced diaminobenzidine (DAB) reaction. Samples can then be analyzed by optical microscopy as well as by electron microscopy.
High molecular weight BDA (10 kDa) yields sensitive and detailed labeling of axons and terminals, while low molecular weight BDA (3 kDa) yields sensitive and detailed retrograde labeling of neuronal cell bodies [1].
References
- ^ Reiner A, Veenman CL, Medina L, Jiao Y, Del Mar N, Honig MG (2000). "Pathway tracing using biotinylated dextran amines". J Neurosci Methods 103 (1): 23–37. doi:10.1016/S0165-0270(00)00293-4. PMID 11074093.
UpToDate Contents
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English Journal
- Glutamatergic Kolliker-Fuse nucleus neurons innervate hypoglossal motoneurons whose axons form the medial (protruder) branch of the hypoglossal nerve in the rat.
- Yokota S, Niu JG, Tsumori T, Oka T, Yasui Y.SourceDepartment of Anatomy and Morphological Neuroscience, Shimane University School of Medicine, Izumo 693-8501, Japan.
- Brain research.Brain Res.2011 Aug 2;1404:10-20. Epub 2011 Jun 17.
- This study was performed to understand the anatomical substrates for Kolliker-Fuse nucleus (KFN) modulation of respiratory-related tongue movement. After application of cholera toxin B subunit (CTb) to the medial branch of the hypoglossal nerve (HGn) and injection of biotinylated dextran amine (BDA
- PMID 21724177
- Pathways of attention: synaptic relationships of frontal eye field to v4, lateral intraparietal cortex, and area 46 in macaque monkey.
- Anderson JC, Kennedy H, Martin KA.SourceInstitute for Neuroinformatics, University of Zurich and ETH Zurich, 8057 Zurich, Switzerland, and Stem Cell and Brain Research Institute, Institute National de la Sante et de la Recherche Medicale U846, 69500 Bron, France and Universite de Lyon, Universite Lyon 1, 69003 Lyon, France.
- The Journal of neuroscience : the official journal of the Society for Neuroscience.J Neurosci.2011 Jul 27;31(30):10872-81.
- The frontal eye field (FEF) of the primate neocortex occupies a pivotal position in the matrix of inter-areal projections. In addition to its role in directing saccadic eye movements, it is the source of an attentional signal that modulates the activity of neurons in extrastriate and parietal cortex
- PMID 21795539
Japanese Journal
- 舌下神経核におけるコリン作動性大型終末“C-terminal”の起始細胞の同定
- 灰塚 嘉典,松井 利康,松村 讓兒,小林 靖
- 杏林医学会雑誌 40(3), 51-65, 2009
- … 次に,その各領域にBiotinylated dextran amineを注入して順行性標識を行い,VAChTに対する免疫組織化学染色との組合せにより,それらの領域がコリン作動性大型終末を舌下神経核に送っているかどうか検証した。 …
- NAID 130000259024
- GABAergic projections from the hippocampus to the retrosplenial cortex in the rat
- Rockland Kathleen S.
- EUROPEAN JOURNAL OF NEUROSCIENCE 26(5), 1193-1204, 2007-9
- … Terminations target layer 1 of the GRS, as shown by biotinylated dextran amine (BDA) injections into CA1 and confirmed by a very superficial injection of CTB-Alexa488 in GRS. …
- NAID 120001371025
Related Links
- Used as anterograde tracers, our Biotinylated Dextran Amines (BDA) are of approximately 10,000 MW and are conjugated with either fluorescein or Texas Red ®. These tracers can be effectively introduced by iontophoretic or ...
- Biotinylated dextran amines (BDA) are organic compounds used as anterograde and retrograde neuroanatomical tracers. They can be used for labeling the source as ... Biotinylated dextran amine s (BDA) are highly sensitive tools for ...
Related Pictures
★リンクテーブル★
[★]
- 関
- biotinylated dextran amine
[★]
- 関
- biotinylation