Flow cytometric assay using two fluorescent proteins for the function of the internal ribosome entry site of hepatitis C virus.
Shi G, Yagyu F, Shimizu Y, Shimizu K, Oshima M, Iwamoto A, Gao B, Liu W, Gao GF, Kitamura Y.SourceChina-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, China.
Cytometry. Part A : the journal of the International Society for Analytical Cytology.Cytometry A.2011 Aug;79(8):653-60. doi: 10.1002/cyto.a.21094. Epub 2011 Jun 27.
The initiation of translation in hepatitis C virus (HCV) occurs at the internal ribosome entry site (IRES) located at the 5'-end of its genomic RNA. To study the function of HCV IRES, we constructed a reporter plasmid that generates a bicistronic mRNA encoding two fluorescent proteins: cap-dependent
Acyl depsipeptide (ADEP) resistance in Streptomyces.
Gominet M, Seghezzi N, Mazodier P.SourceInstitut Pasteur, Unite de Biologie des Bacteries Pathogenes a Gram-Positif, CNRS URA 2172, F-75015 Paris, France.
Microbiology (Reading, England).Microbiology.2011 Aug;157(Pt 8):2226-34. Epub 2011 Jun 2.
ADEP, a molecule of the acyl depsipeptide family, has an antibiotic activity with a unique mode of action. ADEP binding to the ubiquitous protease ClpP alters the structure of the enzyme. Access of protein to the ClpP proteolytic chamber is therefore facilitated and its cohort regulatory ATPases (Cl
A parenteral DNA vaccine protects against pneumonic plague.
Yamanaka Hitoki,Hoyt Teri,Yang Xinghong,Bowen Richard,Golden Sarah,Crist Kathryn,Becker Todd,Maddaloni Massimo,Pascual David W
Vaccine 28(18), 3219-3230, 2010-04-19
… The chemokine, lymphotactin (LTN), was tested as a molecular adjuvant using bicistronic DNA vaccines encoding the protective Yersinia capsular (F1) antigen and virulence antigen (V-Ag) as a F1-V fusion protein. …
Use of bicistronic vectors in combination with flow cytometry to screen for effective small interfering RNA target sequences.
Kamio Naoka,Hirai Hideyo,Ashihara Eishi,Tenen Daniel G,Maekawa Taira,Imanishi Jiro
Biochemical and biophysical research communications 393(3), 498-503, 2010-03-12
… Here, we used a bicistronic vector expressing a target gene and green fluorescent protein (GFP) to create a system in which the effect of an siRNA sequence was reflected in the GFP expression level. … Cells were transduced with the bicistronic vector, expression vectors for siRNA and red fluorescent protein (RFP). …