バクミド
English Journal
- Choristoneura fumiferana multiple nucleopolyhedrovirus LEF-3-P143 complex can complement DNA replication and budded virus in an AcMNPV LEF-3-P143 double knockout bacmid.
- Yu M, Carstens EB.SourceDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada.
- The Journal of general virology.J Gen Virol.2012 Feb;93(Pt 2):383-8. Epub 2011 Nov 2.
- Transient replication assays using Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Choristoneura fumiferana multiple nucleopolyhedrovirus (CfMNPV) genes suggested that the interactions between P143, the viral helicase and LEF-3, a ssDNA-binding protein, may represent virus species
- PMID 22049089
- Autographa californica multiple nucleopolyhedrovirus odv-e25 (Ac94) is required for budded virus infectivity and occlusion-derived virus formation.
- Chen L, Hu X, Xiang X, Yu S, Yang R, Wu X.SourceCollege of Animal Sciences, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China.
- Archives of virology.Arch Virol.2012 Jan 5. [Epub ahead of print]
- Autographa californica multiple nucleopolyhedrovirus (AcMNPV) odv-e25 is a core gene found in all lepidopteran baculoviruses, but its function is unknown. In this study, we generated an odv-e25-knockout AcMNPV and investigated the roles of ODV-E25 in the baculovirus life cycle. The odv-e25 knockout
- PMID 22218963
Japanese Journal
- Phenotypic grouping of 141 BmNPVs lacking viral gene sequences
- Ono Chikako,Kamagata Takanori,Taka Hitomi,Sahara Ken,Asano Shin-ichiro,Bando Hisanori
- Virus Research 165(2), 197-206, 2012-05-00
- … We constructed a series of gene knockout BmNPVs (KOVs) for each of 141 genes using the BmNPV T3 bacmid system and lambda red recombination system. …
- NAID 120004094729
- Production of Rous sarcoma virus-like particles displaying human transmembrane protein in silkworm larvae and its application to ligand-receptor binding assay
- Tsuji Yoshitaka,Deo Vipin Kumar,Kato Tatsuya,Park Enoch Y.
- Journal of biotechnology 155(2), 185-192, 2011-09-10
- … Gag577 was secreted into hemolymph efficiently using wild type bacmid (WT), cysteine protease-deficient bacmid (CP(-)), cysteine protease and chitinase-deficient bacmid (CP(-)Chi(-)) bacmids, but comparatively Gag701 secretion levels were low. …
- NAID 120003256123
- 2Ga04 カイコ幼虫を用いたウイルス様粒子(Virus-like particles)の効率的発現と応用(セル&ティッシュエンジニアリング,一般講演)
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- ・pFastBac™ は、クローニングとバキュロウイルスゲノム(bacmid DNA)への転移に 必要な要素をすべて有する。 ・組換えpFastBac™ 内の発現カセットのbacmid DNAへ の転移は、Tn7部位特異的トランスポジションによって、DH10Bac™ 内で行われる。 特長 ...
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