Characterization of prostaglandin E2 receptors (EP2, EP4) in the horse oviduct.
Ball BA1, Scoggin KE, Troedsson MH, Squires EL.Author information 1Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY 40546, USA. Electronic address: b.a.ball@uky.edu.AbstractBiological effects of prostaglandin E2 are mediated via one of four receptors designated EP1, EP2, EP3 and EP4 which are encoded by separate genes. In general, EP1 and EP3 induce smooth muscle contraction whereas EP2 and EP4 induce smooth muscle relaxation. The objective of the current study was to characterize the expression of the genes for PGE2 receptors (EP2 and EP4) in the horse oviduct based upon immunohistochemistry (IHC) and quantitative PCR (qPCR). Oviductal tissue was collected from mares at estrus (n=5), at 5 days post-ovulation (n=4), and from prepubertal mares (n=5). Isthmic and ampullar regions of the oviduct were fixed for IHC or preserved for RNA isolation. Prostaglandin E2 receptors EP2 and EP4 were strongly expressed by the luminal epithelium of both the isthmic and ampullar regions of the horse oviduct with a lesser immuno-expression noted within the smooth muscle in both regions of the oviduct. Based upon qPCR, relative amounts of EP2 or EP4 mRNA did not differ across estrous cycle stage or from prepubertal mares. However, across region and estrous cycle stage, relative amount of EP2 was greater (P<0.05) than EP4, and relative amount of EP2 mRNA was greater (P<0.001) in the ampullar compared with the isthmic oviduct. The relative roles of these receptors in regulating oviduct function in the mare remains to be determined.
Animal reproduction science.Anim Reprod Sci.2013 Nov 1;142(1-2):35-41. doi: 10.1016/j.anireprosci.2013.07.009. Epub 2013 Aug 12.
Biological effects of prostaglandin E2 are mediated via one of four receptors designated EP1, EP2, EP3 and EP4 which are encoded by separate genes. In general, EP1 and EP3 induce smooth muscle contraction whereas EP2 and EP4 induce smooth muscle relaxation. The objective of the current study was to
Regional differences in myelination of chick vestibulocochlear ganglion cells.
Sun YJ1, Kobayashi H, Yoshida S, Shirasawa N, Naito A.Author information 1Department of Anatomy and Structural Science, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata 990-9585, Japan. Electronic address: sun@med.id.yamagata-u.ac.jp.AbstractIn vertebrates, vestibular and cochlear ganglion (VG and CG, respectively) cells are bipolar neurons with myelinated axons and perikarya. The time course of the myelination of the VG and CG cells during development of chick embryos was investigated. Chick VG and CG from embryonic day at 7-20 (E7-20) were prepared for a transmission electron microscopy, myelin basic protein immunohistochemistry, and real-time quantitative RT-PCR. In the VG cells, myelination was first observed on the peripheral axons of the ampullar nerves at E10, on the utricular and saccular nerves at E12, and on the lagenar and neglecta nerves at E13. In the VG central axons, myelination was first seen on the ampullar nerves at E11, on the utricular and saccular nerves at E13, and on the lagenar nerves at E13. In the CG cells, the myelination was first observed on the peripheral and central axons at E14. In both VG and CG, myelination was observed on the perikarya at E17. These results suggest that the onset of the axonal myelination on the VG cells occurred earlier than that on the CG cells, whereas the perikaryal myelination occurred at about the same time on the both types of ganglion cells. Moreover, the myelination on the ampullar nerves occurred earlier than that on the utricular and saccular nerves. The myelination on the peripheral axons occurred earlier than that on the central axons of the VG cells, whereas that on the central and peripheral axons of the CG cells occurred at about the same time. The regional differences in myelination in relation to the onset of functional activities in the VG and CG cells are discussed.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience.Int J Dev Neurosci.2013 Nov;31(7):568-79. doi: 10.1016/j.ijdevneu.2013.07.001. Epub 2013 Jul 16.
In vertebrates, vestibular and cochlear ganglion (VG and CG, respectively) cells are bipolar neurons with myelinated axons and perikarya. The time course of the myelination of the VG and CG cells during development of chick embryos was investigated. Chick VG and CG from embryonic day at 7-20 (E7-20)
am·pul·lar (am-pul'ăr), Relating in any sense to an ampulla. ampullary adjective Referring to an ampulla, usually understood to mean the ampulla of Vater. am·pul·lar (am-pul'ăr) Relating in any sense to an ampulla. ampullar pertaining ...
In the presented patient the main risk factor was the Mondini's dysplasia, which is a developmental anomaly of the middle ear characterized by cochlear malformation with dilation of the vestibular aquaduct, vestibule and ampullar ends ...