Atomic structure of bovine Arp2/3 complex
[1] (PDB code: 1k8k). Color coding for subunits: Arp3, orange; Arp2, marine (subunits 1 & 2 not resolved and thus not shown); p40, green; p34, ice blue; p20, dark blue; p21, magenta; p16, yellow.
Arp2/3 complex is a seven-subunit protein that plays a major role in the regulation of the actin cytoskeleton. It is a major component of the actin cytoskeleton and is found in most in actin cytoskeleton-containing eukaryotic cells[16]. Two of its subunits, the Actin-Related Proteins ARP2 and ARP3 closely resemble the structure of monomeric actin and serve as nucleation sites for new actin filaments. The complex binds to the sides of existing ("mother") filaments and initiates growth of a new ("daughter") filament at a distinctive 70 degree angle from the mother. Branched actin networks are created as a result of this nucleation of new filaments. The regulation of rearrangements of the actin cytoskeleton is important for processes like cell locomotion, phagocytosis, and intracellular motility of lipid vesicles.
The Arp2/3 complex was named after it was identified by affinity chromatography from Acanthamoeba castellanii[14], though it had been previously isolated in 1989 in a search for proteins that bind to actin filaments in Drosophila melanogaster embryos [15]. It is found in most eukaryotic organisms, but absent from a number of Chromalveolates and plants[16].
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Contents
- 1 Mechanisms of actin polymerization by Arp2/3
- 1.1 Side branching model
- 1.2 Barbed end branching model
- 2 Cellular uses of Arp2/3
- 3 References
- 4 External links
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Mechanisms of actin polymerization by Arp2/3
Side branching model of the Arp2/3 complex. Activated Arp2/3 complex binds to the side of a "mother" actin filament. Both Arp2 and Arp3 form the first two subunits in the new "daughter" filament.
Barbed end branching model of the Arp2/3 complex. Activated Arp2/3 competes with capping proteins to bind to the barbed end of an actin filament. Arp2 remains bound to the mother filament, while Arp3 is outside. The two Arp subunits form the first subunits of each branch and the two branches continue to grow by addition of G-actin to each Arp
Many actin-related molecules create a free barbed end for polymerization by uncapping or severing pre-existing filaments and using these as nucleation cores. However, the Arp2/3 complex stimulates actin polymerization by creating a new nucleation core. Actin nucleation is an initial step in the formation of an actin filament. The nucleation core activity of Arp2/3 is activated by members of the Wiskott-Aldrich syndrome family protein (WASP, N-WASP, WAVE, and WASH proteins). The V domain of a WASP protein interacts with actin monomers while the CA region associates with the Arp2/3 complex to create a nucleation core. However, de novo nucleation followed by polymerization is not sufficient to form integrated actin networks, since these newly synthesized polymers would not be associated with pre-existing filaments. Thus, the Arp2/3 complex binds to pre-existing filaments so that the new filaments can grow on the old ones and form a functional actin cytoskeleton.[2] Capping proteins limit actin polymerization to the region activated by the Arp2/3 complex, and the elongated filament ends are recapped to prevent depolymerization and thus conserve the actin filament.[3]
The Arp2/3 complex simultaneously controls nucleation of actin polymerization and branching of filaments. Moreover, autocatalysis is observed during Arp2/3-mediated actin polymerization. In this process, the newly formed filaments activate other Arp2/3 complexes, facilitating the formation of branched filaments.
The mechanisms of actin polymerization by Arp2/3 has been the subject of dispute in the recent years. The question is where the complex binds the filament and how it nucleates a "daughter" filament. Historically two models have been proposed to describe the formation of branched filaments:
Side branching model
In the side branching (or dendritic nucleation) model, the Arp2/3 complex binds to the side of a pre-existing ("mother") filament at a point different from the nucleation site. Arp2/3 thus has two actin-binding sites — one to bind to the pre-existing actin filament and the other for the nucleation of a branched filament. Recent structural data from high-resolution electron microscopy[4][12], together with biochemical data on the filament nucleation and capping mechanisms of the Arp2/3 complex[13], provide strong support for this model.
Barbed end branching model
In the barbed end branching model, Arp2/3 associates at the barbed end of growing filaments, allowing for the elongation of the original filament and the formation of a branched filament,[2] suggesting that branching is induced with Arp2 and Arp3 being incorporated in two different actin filaments. However, this model is only based on kinetic analysis and lacks support from structural data.
Cellular uses of Arp2/3
The Arp2/3 complex appears to be important in a variety of specialized cell functions that involve the actin cytoskeleton. The complex is found in cellular regions characterized by dynamic actin filament activity; in macropinocytic cups, in the leading edge of motile cells (lamellipodia), and in motile actin patches in yeast.[8] In mammals and the social amoeba Dictyostelium discoideum [9] [10] it is required for phagocytosis. The complex has also been shown to be involved in the establishment of cell polarity and the migration of fibroblast monolayers in a wound-healing model.[11] In mammlian oocytes, Arp2/3 complex involved into the oocyte asymmetric division and polar body emission, which are resulted from the failure of spindle migration (a unique feathure of oocyte division) and cytokinesis. Moreover, enteropathogenic organisms like Listeria monocytogenes and Shigella use the Arp2/3 complex for actin-polymerization dependent rocketing movements. The Arp2/3 complex also regulates the intracellular motility of endosomes, lysosomes, pinocytic vesicles and mitochondria.[6] Moreover, recent studies show that the Arp2/3 complex is essential for proper polar cell expansion in plants. Arp2/3 mutations in Arabidopsis thaliana result in abnormal filament organization, which in turn affects the expansion of trichomes, pavement cells, hypocotyl cells, and root hair cells.[5][7]
References
- a Robinson RC, Turbedsky K, Kaiser DA, et al. (November 2001). "Crystal structure of Arp2/3 complex". Science 294 (5547): 1679–84. doi:10.1126/science.1066333. PMID 11721045. http://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=11721045.
- a b Suetsugu S, Miki H, Takenawa T (March 2002). "Spatial and temporal regulation of actin polymerization for cytoskeleton formation through Arp2/3 complex and WASP/WAVE proteins". Cell Motil. Cytoskeleton 51 (3): 113–22. doi:10.1002/cm.10020. PMID 11921168.
- a Aguda AH, Burtnick LD, Robinson RC (March 2005). "The state of the filament". EMBO Rep. 6 (3): 220–6. doi:10.1038/sj.embor.7400363. PMC 1299273. PMID 15741975. //www.ncbi.nlm.nih.gov/pmc/articles/PMC1299273/.
- a Egile C, Rouiller I, Xu XP, Volkmann N, Li R, Hanein D (November 2005). "Mechanism of filament nucleation and branch stability revealed by the structure of the Arp2/3 complex at actin branch junctions". PLoS Biol. 3 (11): e383. doi:10.1371/journal.pbio.0030383. PMC 1278936. PMID 16262445. http://dx.plos.org/10.1371/journal.pbio.0030383.
- a Bannigan A, Baskin TI (December 2005). "Directional cell expansion—turning toward actin". Curr. Opin. Plant Biol. 8 (6): 619–24. doi:10.1016/j.pbi.2005.09.002. PMID 16181803. http://linkinghub.elsevier.com/retrieve/pii/S1369-5266(05)00124-X.
- a Mathur J (April 2005). "The ARP2/3 complex: giving plant cells a leading edge". BioEssays 27 (4): 377–87. doi:10.1002/bies.20206. PMID 15770684.
- a Xu J, Scheres B (December 2005). "Cell polarity: ROPing the ends together". Curr. Opin. Plant Biol. 8 (6): 613–8. doi:10.1016/j.pbi.2005.09.003. PMID 16182602. http://linkinghub.elsevier.com/retrieve/pii/S1369-5266(05)00127-5.
- a Warren DT, Andrews PD, Gourlay CW, Ayscough KR (April 2002). "Sla1p couples the yeast endocytic machinery to proteins regulating actin dynamics". J. Cell. Sci. 115 (Pt 8): 1703–15. PMID 11950888. http://jcs.biologists.org/cgi/pmidlookup?view=long&pmid=11950888.
- a May RC, Caron E, Hall A, Machesky LM (April 2000). "Involvement of the Arp2/3 complex in phagocytosis mediated by FcgammaR or CR3". Nat. Cell Biol. 2 (4): 246–8. doi:10.1038/35008673. PMID 10783245.
- a Insall R, Müller-Taubenberger A, Machesky L, et al. (November 2001). "Dynamics of the Dictyostelium Arp2/3 complex in endocytosis, cytokinesis, and chemotaxis". Cell Motil. Cytoskeleton 50 (3): 115–28. doi:10.1002/cm.10005. PMID 11807934.
- a Magdalena J, Millard TH, Etienne-Manneville S, Launay S, Warwick HK, Machesky LM (February 2003). "Involvement of the Arp2/3 complex and Scar2 in Golgi polarity in scratch wound models". Mol. Biol. Cell 14 (2): 670–84. doi:10.1091/mbc.E02-06-0345. PMC 150000. PMID 12589062. http://www.molbiolcell.org/cgi/pmidlookup?view=long&pmid=12589062.
- a Volkmann N, Amann KJ, Stoilova-McPhie S, et al. (September 2001). "Structure of Arp2/3 complex in its activated state and in actin filament branch junctions". Science 293 (5539): 2456–9. doi:10.1126/science.1063025. PMID 11533442. http://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=11533442.
- a Dayel MJ, Mullins RD (April 2004). "Activation of Arp2/3 complex: addition of the first subunit of the new filament by a WASP protein triggers rapid ATP hydrolysis on Arp2". PLoS Biol. 2 (4): E91. doi:10.1371/journal.pbio.0020091. PMC 387265. PMID 15094799. http://dx.plos.org/10.1371/journal.pbio.0020091.
- a Machesky LM, Atkinson SJ, Ampe C, Vandekerckhove J, Pollard TD (October 1994). "Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin-agarose". J. Cell Biol. 127 (1): 107–15. doi:10.1083/jcb.127.1.107. PMC 2120189. PMID 7929556. http://www.jcb.org/cgi/pmidlookup?view=long&pmid=7929556.
- a Miller KG, Field CM, Alberts BM (December 1989). "Actin-binding proteins from Drosophila embryos: a complex network of interacting proteins detected by F-actin affinity chromatography". J. Cell Biol. 109 (6 Pt 1): 2963–75. doi:10.1083/jcb.109.6.2963. PMC 2115944. PMID 2512303. http://www.jcb.org/cgi/pmidlookup?view=long&pmid=2512303.
- a Veltman DM, Insall RH (August 2010). "WASP family proteins: their evolution and its physiological implications". Mol. Biol. Cell 21 (16): 2880–93. doi:10.1091/mbc.E10-04-0372. PMC 2921111. PMID 20573979. http://www.molbiolcell.org/cgi/pmidlookup?view=long&pmid=20573979.
External links
- MBInfo - Arp2/3 Mediated Nucleation
- Arp2-3+Complex at the US National Library of Medicine Medical Subject Headings (MeSH)
- Arp2/3 filament nucleation model, illustrated with 2D animations and 3D structure-based movies
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Proteins of the cytoskeleton
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| Nonhuman |
- Major sperm proteins
- Prokaryotic cytoskeleton
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See also: cytoskeletal defects B strc: edmb (perx), skel (ctrs), epit, cili, mito, nucl (chro)
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