English Journal

Revealing Ligand Binding Sites and Quantifying Subunit Variants of Noncovalent Protein Complexes in a Single Native Top-Down FTICR MS Experiment.

Li H1, Wongkongkathep P, Van Orden SL, Ogorzalek Loo RR, Loo JA.
Journal of the American Society for Mass Spectrometry.J Am Soc Mass Spectrom.2014 Jun 10. [Epub ahead of print]
"Native" mass spectrometry (MS) has been proven to be increasingly useful for structural biology studies of macromolecular assemblies. Using horse liver alcohol dehydrogenase (hADH) and yeast alcohol dehydrogenase (yADH) as examples, we demonstrate that rich information can be obtained in a single n
PMID 24912433

Measuring kinetic isotope effects in enzyme reactions using time-resolved electrospray mass spectrometry.

Liuni P1, Olkhov-Mitsel E, Orellana A, Wilson DJ.
Analytical chemistry.Anal Chem.2013 Apr 2;85(7):3758-64. doi: 10.1021/ac400191t. Epub 2013 Mar 20.
Kinetic isotope effect (KIE) measurements are a powerful tool for studying enzyme mechanisms; they can provide insights into microscopic catalytic processes and even structural constraints for transition states. However, KIEs have not come into widespread use in enzymology, due in large part to the
PMID 23461634

Facile fabrication of organic-inorganic hybrid beads by aminated alginate enabled gelation and biomimetic mineralization.

Li J1, Wu H, Liang Y, Jiang Z, Jiang Y, Zhang L.
Journal of biomaterials science. Polymer edition.J Biomater Sci Polym Ed.2013;24(2):119-34. doi: 10.1163/156856212X627351. Epub 2012 May 11.
Inspired by biomineralization, design and preparation of biomimetic organic-inorganic composites have become a hot issue and a research frontier in many areas, including enzyme engineering. In this research, a unique and facile method for fabricating organic-inorganic hybrid beads is proposed. Modif
PMID 22370121

Japanese Journal

Optimization of Adsorptive Immobilization of Alcohol Dehydrogenases(Enzymology, Protein Engineering, and Enzyme Technology)

TRIVEDI ARCHANA,HEINEMANN MATTHIAS,SPIESS ANTJE C.,DAUSSMANN THOMAS,BUCHS JOCHEN
Journal of bioscience and bioengineering 99(4), 340-347, 2005-04-25
… Residual activity of 79% was achieved with ADH from bakers' yeast (YADH) after optimizing the immobilization parameters. … ADH from Thermoanaerobacter species (ADH T) is found to be stable under drying conditions (>15kPa) in contrast to LBADH and YADH. …
NAID 110002695669

DEACTIVATION KINETICS OF YEAST ALCOHOL DEHYDROGENZSE IN AEROSOL OT/ISOOCTANE REVERSE MICELLES

CHEN Dong-Hwang,CHEN Hsien-Hsin,HUANG Ting-Chia
Journal of chemical engineering of Japan 28(5), 551-555, 1995-10
… The deactivation kinetics of yeast alcohol dehydrogenase (YADH) in both aerosol OT/isooctane reverse micelles and aqueous buffer were studied. … The YADH entrapped in reverse micelles; … Both the activity-time curves for the YADH in reverse micelles and in aqueous buffer exhibited a rather rapid exponential decay within the early 2 hr, followed by a slower exponential decay during the remaining period. …
NAID 130000021132

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