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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/11/05 10:29:13」(JST)

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Tricine is an organic compound that is used in buffer solutions. The name tricine comes from tris and glycine, from which it was derived.^[1] It is a white crystalline powder that is moderately soluble in water. It is a zwitterionic amino acid that has a pKa1 value of 2.3 at 25 °C, while its pKa2 at 20 °C is 8.15. Its useful buffering range of pH is 7.4-8.8. Along with bicine, it is one of Good's buffering agents. Good first prepared tricine to buffer chloroplast reactions.

Applications

Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets. It has a higher negative (more negative) charge than glycine allowing it to migrate faster. In addition its high ionic strength causes more ion movement and less protein movement. This allows for low molecular weight proteins to be separated in lower percent acrylamide gels. Tricine has been documented in the separation of proteins in the range of 1 to 100 kDa by electrophoresis.^[2] The tricine buffer at 25 mmol/L was found to be the most effective buffer among the ten tested for ATP assays using firefly luciferase.^[3] Tricine has also been found to be an effective scavenger of hydroxyl radicals in a study of radiation-induced membrane damage.^[4]

References

^ Good, N.E., et al., Biochemistry, v. 5, 467 (1966).
^ Schaegger, H., and von Jagow, G., "Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa." "Anal. Biochem." 166(2), 368-379.
^ Webster, J. J., and Leach, F. R., "Optimization of the firefly luciferase assay for ATP." "J. Appl. Biochem.", 2:469-479.
^ Hicks, M., and Gebicki, J. M., "Rate constants for reaction of hydroxyl radicals with Tris, Tricine, and Hepes buffers." "FEBS Lett.", 199(1):92-94.

English Journal

Comparison of inducible versus constitutive expression of plectasin on yields and antimicrobial activities in Pichia pastoris.

Chen X1, Hu YH2, Chen WD2, Li WD2, Huang ZC2, Li Y1, Luo YW1, Huang YX1, Chen YT1, Wang K1, Li L3.
Protein expression and purification.Protein Expr Purif.2016 Feb;118:70-6. doi: 10.1016/j.pep.2015.10.010. Epub 2015 Oct 20.
BACKGROUND: Plectasin might serve as a substitute for traditional antibiotics, but its yields and antimicrobial activities warrant further investigation.OBJECTIVE: To identify the influence of inducible versus constitutive expression of plectasin on yields and antimicrobial activities.METHODS: Throu
PMID 26500192

Construction of Recombinant Pichia pastoris Carrying a Constitutive AvBD9 Gene and Analysis of Its Activity.

Tu J1, Qi K1, Xue T1, Wei H1, Zhang Y1, Wu Y1, Zhou X1, Lv X1.
Journal of microbiology and biotechnology.J Microbiol Biotechnol.2015 Dec 28;25(12):2082-9. doi: 10.4014/jmb.1506.06056.
Avian beta-defensin 9 (AvBD9) is a small cationic peptide consisting of 41 amino acids that plays a crucial rule in innate immunity and acquired immunity in chickens. Owing to its wide antibacterial spectrum, lack of a residue, and failure to induce bacterial drug resistance, AvBD9 is expected to be
PMID 26370794

Microcin V Production in Lactobacillus plantarum LB-B1 Using Heterologous Leader Peptide from Pediocin PA-1.

Ma X1, Wang G1, Li D1, Hao Y2.
Current microbiology.Curr Microbiol.2015 Dec 19. [Epub ahead of print]
Lactobacillus strains producing bacteriocins have attracted highly attention as probiotic cultures in animal nutrition since the use of antibiotics was forbidden in the livestock industry. Lactobacillus plantarum LB-B1 isolated from the fermented dairy product can produce pediocin PA-1, which has a
PMID 26687333

Japanese Journal

A new approach to detect small peptides clearly and sensitively by Western blotting using a vacuum-assisted detection method

Tomisawa Satoshi,Abe Chiharu,Kamiya Masakatsu,Kikukawa Takashi,Demura Makoto,Kawano Keiichi,Aizawa Tomoyasu
BIOPHYSICS 9(0), 79-83, 2013
Western blotting is a widely used technique for the detection and quantification of proteins and peptides. However, it is challenging to detect small peptides efficiently by the conventional Western b …
NAID 130003366033

Establishment and application of a high-quality comparative analysis strategy for the discovery and small-scale validation of low-abundance biomarker peptides in serum based on an optimized novel peptide extraction method

Saito Tatsuya,Kawashima Yusuke,Minamida Satoru,Matsumoto Kazumasa,Araki Keita,Matsui Takashi,Satoh Mamoru,Nomura Fumio,Iwamura Masatsugu,Maeda Tadakazu,Baba Shiro,Kodera Yoshio
Journal of Electrophoresis 57(1), 1-9, 2013
… We optimized our novel differential solubilization (DS) peptide extraction method to selectively enrich peptides less than 6 kDa, using tricine-SDS-PAGE to evaluate the optimization. …
NAID 130003364779

Gentian lipid transfer protein homolog with antimicrobial properties confers resistance to Botrytis cinerea in transgenic tobacco

Kiba Akinori,Nakatsuka Takashi,Yamamura Saburo [他],NISHIHARA Masahiro
Plant biotechnology 29(1), 95-101, 2012-03-25
… 8.0 K (8.0 KP) and 7.0 K (7.0 KP) were detected by Tricine-SDS PAGE under reducing conditions. …
NAID 10030561589

「トリシン」

Tricine
Names
	IUPAC name N-(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)glycine
	Other names Tricine; N-(Tri(hydroxymethyl)methyl)glycine
Identifiers
CAS Registry Number	5704-04-1
Beilstein Reference	1937804
ChEBI	CHEBI:39063
ChemSpider	72078
EC number	227-193-6
Gmelin Reference	3688
	InChI InChI=1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12) ; Key: SEQKRHFRPICQDD-UHFFFAOYSA-N ;
Jmol-3D images	Image; Image
MeSH	tricine
PubChem	[http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=79784 Template:Pubchemcie 79784 Template:Pubchemcie]
	SMILES OCC(CO)(CO)NCc(:[o]):[oH] ; OCC(CO)(CO)NCC(O)=O ;
Properties
Chemical formula	C6H13NO5
Molar mass	179.17 g·mol−1
Appearance	White crystals
Solubility in water	89.6 g L−1 (at 20 °C)
UV-vis (λmax)	260 nm
Absorbance	0.03
Related compounds
Related compounds	Milacemide
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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	Infobox references

　　[★]

英: Tricine、N-tris(hydroxymethyl)methylglycine
関: N-トリスヒドロキシメチルメチルグリシン

pH緩衝剤

「N-tris(hydroxymethyl)methylglycine」

　　[★] N-トリスヒドロキシメチルメチルグリシン、トリシン

関: Tricine

[Good-1] Good, N.E., et al., Biochemistry, v. 5, 467 (1966).

[2] Schaegger, H., and von Jagow, G., "Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa." "Anal. Biochem." 166(2), 368-379.

[3] Webster, J. J., and Leach, F. R., "Optimization of the firefly luciferase assay for ATP." "J. Appl. Biochem.", 2:469-479.

[4] Hicks, M., and Gebicki, J. M., "Rate constants for reaction of hydroxyl radicals with Tris, Tricine, and Hepes buffers." "FEBS Lett.", 199(1):92-94.

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Tricine