シネコシスティス、シネコシスチス、ネコシスティス属、Synechocystis属

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2012/11/05 15:13:51」(JST)

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Synechocystis sp. PCC6803 is a freshwater cyanobacterium capable of both phototrophic growth by oxygenic photosynthesis in sunlight and heterotrophic growth by glycolysis and oxidative phosphorylation during dark periods. Transitions of light and dark phases are effectively anticipated by a circadian clock.

Overview

Cyanobacteria are model microorganisms for the study of photosynthesis, carbon and nitrogen assimilation, evolution of plant plastids, and adaptability to environmental stresses. Synechocystis sp. PCC 6803 is one of the most highly studied cyanobacteria as it can grow both autotrophically or heterotrophically in the absence of light. It was isolated from a freshwater lake in 1968 and is easily transformed by exogenous DNA. The photosynthetic apparatus is very similar to the one found in plants. This organism also exhibits phototactic movement.

Light-activated heterotrophy

It can live completely heterotrophically in the dark, but for yet unknown reasons requires a minimum of 5 to 15 minutes (blue) light per day. This regulatory role of light is intact in both PSI and PSII deficient strains.^[1]

NDH-2 is a regulatory quinone:NAD(P)H oxidoreductase^[2] global analysis of circadian gene expression indicates that translation genes are expressed at the early subjective day.^[3]

Some glycolytic genes are regulated by sll1330 under light and glucose-supplemented conditions. One of the most important glycolytic genes is fructose-1,6-bisphosphate aldolase (fbaA). The mRNA level of fbaA is increased under light and glucose-supplemented conditions. But in Δsll1330, fbaA is not increased under same conditions.^[4]

References

^ Anderson SL and McIntosh L (May 1991). "Light-activated heterotrophic growth of the cyanobacterium Synechocystis sp. strain PCC 6803: a blue-light-requiring process". J Bacteriol 173 (9): 2761–2767. PMC 207855. PMID 1902208. //www.ncbi.nlm.nih.gov/pmc/articles/PMC207855/.
^ Howitt CA, Udall PK, and Vermaas WF (July 1999). "Type 2 NADH dehydrogenases in the cyanobacterium Synechocystis sp. strain PCC 6803 are involved in regulation rather than respiration". J Bacteriol 181 (13): 3994–4003. PMC 93889. PMID 10383967. //www.ncbi.nlm.nih.gov/pmc/articles/PMC93889/.
^ Kucho K, Okamoto K, Tsuchiya Y, Nomura S, Nango M, Kanehisa M, and Ishiura M (March 2005). "Global analysis of circadian expression in the cyanobacterium Synechocystis sp. strain PCC 6803". J Bacteriol 187 (6): 2190–2199. doi:10.1128/JB.187.6.2190-2199.2005. PMC 1064041. PMID 15743968. //www.ncbi.nlm.nih.gov/pmc/articles/PMC1064041/.
^ Yusuke Tabei, Katsuhiko Okada and Mikio Tsuzuki (April 2007). "Sll1330 controls the expression of glycolytic genes in Synechocystis sp. PCC 6803". Biochem. Biophys. Res. Commun. 355 (4): 1045–1050. doi:10.1016/j.bbrc.2007.02.065. PMID 17331473.

Databases

SynechoNET: integrated protein-protein interaction database of a model cyanobacterium Synechocystis sp. PCC 6803. SynechoNET is a specialized cyanobacterial protein-protein interaction database. It shows feasible cyanobacterial domain-domain interactions, as well as their protein level interactions using the model cyanobacterium, Synechocystis sp. PCC 6803. Additionally, SynechoNET provides transmembrane topology and domain information, as well as the interaction networks in graphical web interfaces.
CyanoBase: Cyanobacteria carry a complete set of genes for oxygenic photosynthesis, which is the most fundamental life process on the earth. This organism is also interesting from an evolutional viewpoint, for it was born in a very ancient age and has survived in various environments. Chloroplast is believed to have evolved from cyanobacterial ancestors which developed an endosymbiontic relationship with a eukaryotic host cell. CyanoBase provides an easy way of accessing the sequences and all-inclusive annotation data on the structures of the cyanobacterial genomes. This database was originally developed by Makoto Hirosawa, Takakazu Kaneko and Satoshi Tabata, and the current version of CyanoBase has been developed and maintained by Yasukazu Nakamura, Takakazu Kaneko, and Satoshi Tabata at Kazusa DNA Research Institute.
STRING: STRING is a database of known and predicted protein-protein interactions.The interactions include direct (physical) and indirect (functional) associations; they are derived from four sources: Genomic Context, High-throughpot Experiments, (Conserved) Coexpression, and Previous Knowledge. The database currently contains 1,513,782 proteins in 373 species. Especially, the database provides interactions for Synechocystis sp. PCC 6803.
cTFbase: cTFbase contains 1288 putative TFs identified from 21 fully sequenced cyanobacterial genomes. Through its user-friendly interactive interface, users can employ various criteria to retrieve all TF sequences and their detailed annotation information, including sequence features, domain architecture and sequence similarity against the linked databases. Furthermore, cTFbase also provides phylogenetic trees of individual TF family, multiple sequence alignments of the DNA-binding domain and ortholog identification from any selected genomes.

English Journal

Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria.

Taton A1, Unglaub F2, Wright NE2, Zeng WY2, Paz-Yepes J3, Brahamsha B4, Palenik B4, Peterson TC5, Haerizadeh F5, Golden SS2, Golden JW2.
Nucleic acids research.Nucleic Acids Res.2015 Jan 1;42(17):e136. doi: 10.1093/nar/gku673. Epub 2014 Jul 29.
Inspired by the developments of synthetic biology and the need for improved genetic tools to exploit cyanobacteria for the production of renewable bioproducts, we developed a versatile platform for the construction of broad-host-range vector systems. This platform includes the following features: (i
PMID 25074377

Regulation of gene expression in diverse cyanobacterial species by using theophylline-responsive riboswitches.

Ma AT1, Schmidt CM1, Golden JW2.
Applied and environmental microbiology.Appl Environ Microbiol.2014 Nov 1;80(21):6704-13. doi: 10.1128/AEM.01697-14. Epub 2014 Aug 22.
Cyanobacteria are photosynthetic bacteria that are currently being developed as biological production platforms. They derive energy from light and carbon from atmospheric carbon dioxide, and some species can fix atmospheric nitrogen. One advantage of developing cyanobacteria for renewable production
PMID 25149516

Removal of both Ycf48 and Psb27 in Synechocystis sp. PCC 6803 disrupts Photosystem II assembly and alters QA(-) oxidation in the mature complex.

Jackson SA1, Hervey JR1, Dale AJ1, Eaton-Rye JJ2.
FEBS letters.FEBS Lett.2014 Oct 16;588(20):3751-60. doi: 10.1016/j.febslet.2014.08.024. Epub 2014 Aug 27.
The Photosystem II (PS II) assembly factors Psb27 and Ycf48 are transiently associated with PS II during its biogenesis and repair pathways. We investigated the function of these proteins by constructing knockout mutants in Synechocystis sp. PCC 6803. In ΔYcf48 cells, PS II electron transfer and st
PMID 25171861

Japanese Journal

Light-Induced Conformational Change and Transient Dissociation Reaction of the BLUF Photoreceptor Synechocystis PixD (Slr1694).

Tanaka Keisuke,Nakasone Yusuke,Okajima Koji,Ikeuchi Masahiko,Tokutomi Satoru,Terazima Masahide
Journal of molecular biology 409(5), 773-785, 2011-06-24
… The light-induced reaction of the BLUF (blue light photoreceptor using flavin adenine dinucleotide) photoreceptor PixD from Synechocystis sp. …
NAID 120003203917

A low molecular weight protein tyrosine phosphatase from Synechocystis sp. strain PCC 6803: enzymatic characterization and identification of its potential substrates

Mukhopadhyay Archana,Kennelly Peter J.
Journal of Biochemistry 149(5), 551-562, 2011-05
NAID 40018787446

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「シネコシスチス」

Synechocystis sp. PCC6803
Scientific classification
Kingdom:	Bacteria
Phylum:	Cyanobacteria
Order:	Chroococcales
Genus:	Synechocystis
Species:	S. sp. PCC6803
Binomial name
	Synechocystis sp.;