- 関
- staphylococcal bacteriophage、staphylococcal phage
WordNet
- spherical Gram-positive parasitic bacteria that tend to form irregular colonies; some cause boils or septicemia or infections (同)staphylococci, staph
PrepTutorEJDIC
- ブドウ状球菌
- =bacteriophage
UpToDate Contents
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English Journal
- Prolonged outbreak of Staphylococcus aureus surgical site infection traced to a healthcare worker with psoriasis.
- Crusz SA1, Yates C1, Holden S1, Kearns A2, Boswell T3.Author information 1Department of Clinical Microbiology, Nottingham University Hospital, Nottingham, UK.2Antimicrobial Resistance and Healthcare Associated Infections Reference Unit, Public Health England, Colindale, London, UK.3Department of Clinical Microbiology, Nottingham University Hospital, Nottingham, UK. Electronic address: tim.boswell@nuh.nhs.uk.AbstractBACKGROUND: Meticillin-sensitive Staphylococcus aureus (MSSA) is a frequent cause of surgical site infection (SSI), but point-source outbreaks are rarely recognized.
- The Journal of hospital infection.J Hosp Infect.2014 Jan;86(1):42-6. doi: 10.1016/j.jhin.2013.10.006. Epub 2013 Oct 31.
- BACKGROUND: Meticillin-sensitive Staphylococcus aureus (MSSA) is a frequent cause of surgical site infection (SSI), but point-source outbreaks are rarely recognized.AIM: To describe an outbreak of MSSA SSI in a thoracic surgical unit.METHODS: An outbreak investigation was started following two posto
- PMID 24296162
- Bacteriophage T4 capsid packaging and unpackaging of DNA and proteins.
- Mullaney JM, Black LW.Author information Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA.AbstractBacteriophage T4 has proven itself readily amenable to phage-based DNA and protein packaging, expression, and display systems due to its physical resiliency and genomic flexibility. As a large dsDNA phage with dispensable internal proteins and dispensable outer capsid proteins it can be adapted to package both DNA and proteins of interest within the capsid and to display peptides and proteins externally on the capsid. A single 170 kb linear DNA, or single or multiple copies of shorter linear DNAs, of any sequence can be packaged by the large terminase subunit in vitro into protein-containing proheads and give full or partially full capsids. The prohead receptacles for DNA packaging can also display peptides or full-length proteins from capsid display proteins HOC and SOC. Our laboratory has also developed a protein expression, packaging, and processing (PEPP) system which we have found to have advantages over mammalian and bacterial cell systems, including high yield, increased stability, and simplified downstream processing. Proteins that we have produced by the phage PEPP platform include human HIV-1 protease, micrococcal endonuclease from Staphylococcus aureus, restriction endonuclease EcoRI, luciferase, human granulocyte colony stimulating factor (GCSF), green fluorescent protein (GFP), and the 99 amino acid C-terminus of amyloid precursor protein (APP). Difficult to produce proteins that are toxic in mammalian protein expression systems are easily produced, packaged, and processed with the PEPP platform. APP is one example of such a highly refractory protein that has been produced successfully. The methods below describe the procedures for in vitro packaging of proheads with DNA and for producing recombinant T4 phage that carry a gene of interest in the phage genome and produce and internally package the corresponding protein of interest.
- Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2014;1108:69-85. doi: 10.1007/978-1-62703-751-8_5.
- Bacteriophage T4 has proven itself readily amenable to phage-based DNA and protein packaging, expression, and display systems due to its physical resiliency and genomic flexibility. As a large dsDNA phage with dispensable internal proteins and dispensable outer capsid proteins it can be adapted to p
- PMID 24243241
- Methicillin Susceptible Staphylococcus aureus (MSSA) of Clonal Complex CC398, t571 from Infections in Humans Are Still Rare in Germany.
- Cuny C1, Layer F1, Köck R2, Werner G1, Witte W3.Author information 1National Reference Center for Staphylococci and Enterococci, Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany.2Institute of Hygiene, University Hospital Münster, Münster, Germany.3Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany.AbstractMethicillin-susceptible Staphylococcus aureus (MSSA) attributed to clonal complex (CC) 398 and exhibiting spa-type t571 received attention in Europe and in the USA for being associated with severe infections in humans. As this spa-type is exhibited by livestock-associated (LA) Methicillin-resistant S. aureus (MRSA) as well, it is important to discriminate LA- and human-derived strains by easy to perform, PCR-based methods. MSSA t571 contain phage int3 carrying scn and chp, whereas LA-MRSA t571 lack these markers. In contrast, pathogenicity island SaPIbov5 (detected by PCR bridging vwbbov and scn) is contained by LA-MRSA t571 and absent in the human MSSA subpopulation. Furthermore, MSSA t571 contain erm(T), the particular genomic arrangement of which was assessed by a PCR bridging erm(T) and the adjacent transposase gene. MSSA t571 are rare so far in Germany among isolates from infections in humans (0.14%) as well as among isolates from nasal colonization (0.13%). LA-MRSA t571 are also infrequent among MRSA isolated from carriage at admission to hospitals (0.1%) and also among isolates from infections in humans (0.013%).
- PloS one.PLoS One.2013 Dec 18;8(12):e83165. doi: 10.1371/journal.pone.0083165.
- Methicillin-susceptible Staphylococcus aureus (MSSA) attributed to clonal complex (CC) 398 and exhibiting spa-type t571 received attention in Europe and in the USA for being associated with severe infections in humans. As this spa-type is exhibited by livestock-associated (LA) Methicillin-resistant
- PMID 24367584
Japanese Journal
- ITS-PCR法およびPOT法によるMRSAの疫学解析に関する検討
- 千田 靖子,竹森 優喜子,岩田 恭宜 [他]
- 臨床病理 : 日本臨床検査医学会誌 62(5), 421-426, 2014-05
- NAID 40020119686
- Genotyping of skin and soft tissue infection (SSTI)-associated methicillin-resistant Staphylococcus aureus (MRSA) strains among outpatients in a teaching hospital in Japan : application of a phage-open reading frame typing (POT) kit
- MAEDA Tadashi,SAGA Tomoo,MIYAZAKI Taito,KOUYAMA Yuichi,HARADA Sohei,IWATA Morihiro,YOSHIZAWA Sadako,KIMURA Soichiro,ISHII Yoshikazu,URITA Yoshihisa,SUGIMOTO Motonobu,YAMAGUCHI Keizo,TATEDA Kazuhiro
- Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy 18(6), 906-914, 2012-12-01
- NAID 10031143894
- MRSAの院内伝播制御に有用なPOT法を用いた分子疫学解析
- 森山 英彦,松田 親史,柴田 宏 [他],西村 信弘,廣瀬 昌博,長井 篤
- 感染症学雑誌 : 日本伝染病学会機関誌 : the journal of the Japanese Association for Infectious Diseases 86(2), 115-120, 2012-03-20
- MRSA の院内伝播を防止するには，サーベイランスが重要であり，感染源の特定や感染経路の究明には分子疫学解析法 （PFGE 法） が用いられてきた．しかし本法は処理時間が長く煩雑なことから，日常業務への導入は困難であった．今回，短時間で再現性良く測定可能な POT 法による分子疫学解析キットが開発されたため，2010 年入院患者から新規に検出された MRSA 192 例について解析を行い，院内伝 …
- NAID 10031120943
Related Links
- [Increasing the effectiveness of the method of Staphylococcus phage-typing] Vopr Pitan. 1989 Mar-Apr; (2):51-4. [Vopr Pitan. 1989] [Phage typing of 100 strains of Staphylococcus aureus isolated in a hospital. Relation to origin and ...
- Name of Bacteriophage and Therapeutic Effect Contents Possible Diseases and Areas to Apply Staphylococcus Bacteriophage (monophage) Suppress bacterial flora, resistant to antibiotic Staphylococcus phage
★リンクテーブル★
[★]
- 英
- staphylococcal phage、Staphylococcus phage
- 関
- ブドウ球菌バクテリオファージ、Staphylococcusファージ、スタフィロコッカスファージ
[★]
- 関
- staphylococcal phage、Staphylococcus bacteriophage、Staphylococcus phage
[★]
- 英
- Staphylococcus phage
- 関
- ブドウ球菌ファージ、スタフィロコッカスファージ
[★]
- 英
- Staphylococcus phage
- 関
- ブドウ球菌ファージ、Staphylococcusファージ
[★]
- 関
- staphylococcal bacteriophage、Staphylococcus phage
[★]
スタフィロコッカス属、ブドウ球菌
[★]
- 関
- bacteriophage