セファロース
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- agarose
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出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2015/07/31 02:49:34」(JST)
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Sepharose is a tradename for a crosslinked, beaded-form of agarose, a polysaccharide polymer material extracted from seaweed. Iodoacetyl functional groups can be added to selectively bind cysteine side chains and this method is often used to immobilize peptides. Its brand name is derived from Separation-Pharmacia-Agarose. A common application for the material is in chromatographic separations of biomolecules.
Sepharose is a registered trademark of GE Healthcare (formerly: Pharmacia, Pharmacia LKB Biotechnology, Pharmacia Biotech, Amersham Pharmacia Biotech, and Amersham Biosciences).
Sepharose/agarose, combined with some form of activation chemistry, is also used to immobilize enzymes, antibodies and other proteins and peptides through covalent attachment to the resin.[1] Common activation chemistries include cyanogen bromide (CNBr) activation and reductive amination of aldehydes to attach proteins to the agarose resin through lysine side chains.
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English Journal
- Analysis of Smad Phosphatase Activity In Vitro.
- Shen T1,2, Qin L2, Lin X3.
- Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2016;1344:111-9. doi: 10.1007/978-1-4939-2966-5_6.
- Phosphorylation of Smad1/5/8 at the C-terminal SXS motif by BMP type I receptors is one of the most critical events in BMP signaling. Conversely, protein phosphatases that dephosphorylate phospho-Smad1/5/8 can consequently prevent or terminate BMP signaling. PPM1H is an undercharacterized phosphatas
- PMID 26520120
- Structural characterization and antioxidant activity of a novel heteropolysaccharide from the submerged fermentation mycelia of Ganoderma capense.
- Huang Y1, Li N2, Wan JB3, Zhang D2, Yan C4.
- Carbohydrate polymers.Carbohydr Polym.2015 Dec 10;134:752-60. doi: 10.1016/j.carbpol.2015.08.067. Epub 2015 Aug 28.
- A novel heteropolysaccharide (GCPB-2) with a molecular mass of 1.03×10(5)Da was isolated from the submerged fermentation culturing mycelia powder of Lingzhi, Ganoderma capense, by DEAE-52 cellulose, DEAE Sepharose CL-6B, and Sephadex G-75 column chromatography. Its chemical structure was characteri
- PMID 26428182
- MDR-TB Antibody Response (Western Blot) to Fractions of Isoniazid and Rifampicin Resistant Antigens of Mycobacterium tuberculosis.
- Hadizadeh Tasbiti A1, Yari S1, Ghanei M1,2, Shokrgozar MA3, Bahrmand A4.
- Current microbiology.Curr Microbiol.2015 Dec;71(6):638-42. doi: 10.1007/s00284-015-0891-x. Epub 2015 Aug 28.
- Drug-resistant TB poses a major threat to control of TB worldwide. Despite progress in the detection of Multidrug-resistant TB (MDR-TB) cases, a major diagnostic gap remains: 55 % of reported TB patients estimated to have MDR-TB were not detected in 2013. MDR-TB antigens were conjugated to CNBr-act
- PMID 26316231
Japanese Journal
- The First Aptamer-Apheresis Column Specifically for Clearing Blood of β1-Receptor Autoantibodies : A Successful Proof of Principle Using Autoantibody-Positive SHR Rats
- WALLUKAT Gerd,HABERLAND Annekathrin,BERG Sabine,SCHULZ Angela,FREYSE Ernst-Joachim,DAHMEN Claudia,KAGE Andreas,DANDEL Michael,VETTER Roland,SALZSIEDER Eckhard,KREUTZ Reinhold,SCHIMKE Ingolf
- Circulation journal : official journal of the Japanese Circulation Society 76(10), 2449-2455, 2012-09-25
- … Methods and Results: An apheresis column was designed containing the β1-AAB-targeting-aptamer coupled to sepharose. …
- NAID 10030875128
- ホタテガイの中腸腺タンパク質と重金属イオンとの親和性
- 糠塚 いそし,藤江 紗代
- 分析化学 = Japan analyst 61(9), 791-795, 2012-09-05
- … Next, we studied the affinity of Cd2+, Cu2+, Ni2+, and Pb2+ to the protein immobilized on Sepharose (p-Sepharose). …
- NAID 10030877363
- Catalysis and stability of an alkaline protease from a haloalkaliphilic bacterium under non-aqueous conditions as a function of pH, salt and temperature(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Pandey Sandeep,Rakholiya Kalpna D.,Raval Vikram H. [他],Singh Satya P.
- Journal of bioscience and bioengineering 114(3), 251-256, 2012-09-00
- … The alkaline protease was purified in a single step on phenyl sepharose 6 FF with 28% yield. …
- NAID 110009517373
Related Links
- Sepharose Fast Flow(FF)イオン交換クロマトグラフィー用担体は、Sepharose CL-6B イオン交換担体よりも架橋度を増して物理的、化学的に強化した高性能イオン交換担体 です。Fast Flow担体は、Sepharose HPの約5倍の流速で担体を再生できるため、 ...
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