シナプトソーム関連タンパク質25、シナプトソーム結合タンパク質25

関: synaptosomal-associated protein 25

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/06/28 11:29:41」(JST)

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Synaptosomal-associated protein 25 (SNAP-25) is a t-SNARE protein that is encoded by the SNAP25 gene in humans.^[1] SNAP-25 is a component of the trans-SNARE complex, which is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together.^[2]

Structure and function

Molecular machinery driving exocytosis in neuromediator release. The core SNARE complex is formed by four α-helices contributed by synaptobrevin, syntaxin and SNAP-25, synaptotagmin serves as a Ca²⁺ sensor and regulates intimately the SNARE zipping.^[3]

SNAP-25, a Q-SNARE protein, is anchored to the cytosolic face of membranes via palmitoyl side chains covalently bound to cysteine amino acid residues in the middle of the molecule. This means that SNAP-25 does not contain a trans-membrane domain.^[4]

SNAP-25 has been identified in contributing two α-helices to the SNARE complex, a four-α-helix domain complex.^[5] The SNARE complex participates in vesicle fusion, which involves the docking and merging of a vesicle with the cell membrane to bring about an exocytotic event. Synaptobrevin, a protein that is a part of the vesicle-associated membrane protein (VAMP) family, and syntaxin-1 also help form the SNARE complex by each contributing one α-helix. SNAP-25 assembles with synaptobrevin and syntaxin-1 and the selective binding of these proteins enables vesicle docking and fusion to occur at the correct location.^[6]

To form the SNARE complex, synaptobrevin, syntaxin-1, and SNAP-25 associate and begin to wrap around each other to form a coiled coil quaternary structure. The α-helices of both synaptobrevin and syntaxin-1 bind to those of SNAP-25. Synaptobrevin binds the α-helix near SNAP-25's C-terminal side, while syntaxin-1 binds the α-helix near the N-terminus.^[4]

SNAP-25 inhibits presynaptic P-, Q-, and L-type voltage-gated calcium channels^[7] and interacts with the synaptotagmin C2B domain in Ca²⁺-independent fashion.^[8] In glutamatergic synapses, SNAP-25 decreases the Ca²⁺ responsiveness, while it is naturally absent in GABAergic synapses.^[9]

Two isoforms (mRNA splice variants) of SNAP-25 exist, which are labeled A and B. There are nine amino acid residue differences between the two isoforms, including a re-localization of one of the four cysteine residues.^[10] The major characteristics of these two forms are outlined in the table below.

	SNAP25A	SNAP25B
Structure	N-terminal α-helix Random coil linker region with four cysteines clustered towards the center C-terminal α-helix	N-terminal α-helix Random coil linker region with four cysteines clustered towards the C-terminus C-terminal α-helix
Expression	Major SNAP-25 isoform in embryos and developing neural tissue Minimal expression in adult tissue except in pituitary and adrenal gland tissues	Minimal expression during development, major isoform in adult neural tissue^[11]
Localization	Diffuse	Localized to terminals and varicosities^[11]

Clinical significance

Consistent with the regulation of synaptic Ca²⁺ responsiveness, heterozygous deletion of the SNAP-25 gene in mice results in a hyperactive phenotype similar to attention deficit hyperactivity disorder (ADHD). In heterozygous mice, a decrease in hyperactivity is observed with dextroamphetamine (or Dexedrine), an active ingredient in the ADHD drug Adderall. Homozygous deletions of the SNAP-25 gene are lethal. Subsequent studies have suggested that at least some of the SNAP-25 gene mutations in humans might predispose to ADHD.^[12]^[13]

A genome wide association study pointed to the rs362584 polymorphism in the gene as possibly associated with the personality trait neuroticism.^[14] Botulinum toxins A, C and E cleave SNAP-25^[15] leading to paralysis in clinically developed botulism.

Interactive pathway map

Click on genes, proteins and metabolites below to link to respective articles. ^{[§ 1]}

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|{{{bSize}}}px|alt=Nicotine Activity on Dopaminergic Neurons edit]]

File:NicotineDopaminergic_WP1602.png

Nicotine Activity on Dopaminergic Neurons edit

^ The interactive pathway map can be edited at WikiPathways: "NicotineDopaminergic_WP1602".

Interactions

SNAP-25 has been shown to interact with:

CPLX1,^[16]^[17]
ITSN1,^[18]
KIF5B,^[19]
SNAPAP^[20] and
STX11,^[21]^[22]
STX1A,^[16]^[20]^[21]^[23]^[24]^[25]^[26]^[27]^[28]^[29]^[30]
STX2,^[23]^[24]
STX4,^[23]^[24]^[25]^[31]
SYT1,^[32]^[33]
Syntaxin 3,^[23]^[24]^[25]
TRIM9,^[29] and
VAMP2.^[16]^[29]^[34]

References

^ Maglott DR, Feldblyum TV, Durkin AS, Nierman WC (May 1996). "Radiation hybrid mapping of SNAP, PCSK2, and THBD (human chromosome 20p)". Mamm. Genome 7 (5): 400–1. doi:10.1007/s003359900120. PMID 8661740.
^ Rizo J, Südhof TC (2002). "Snares and Munc18 in synaptic vesicle fusion". Nat Rev Neurosci 3 (8): 641–653. doi:10.1038/nrn898. PMID 12154365.
^ Georgiev, Danko D; James F . Glazebrook (2007). "Subneuronal processing of information by solitary waves and stochastic processes". In Lyshevski, Sergey Edward. Nano and Molecular Electronics Handbook. Nano and Microengineering Series. CRC Press. pp. 17–1–17–41. ISBN 978-0-8493-8528-5.
^ ^a ^b Chapman ER, An S, Barton N, Jahn R (1994). "SNAP-25, a t-SNARE which binds to both syntaxin and synaptobrevin via domains that may form coiled coils". J. Biol. Chem. 269 (44): 27427–32. PMID 7961655.
^ Pevsner J, Hsu SC, Braun JE, Calakos N, Ting AE, Bennett MK et al. (1994). "Specificity and regulation of a synaptic vesicle docking complex". Neuron 13 (2): 353–61. doi:10.1016/0896-6273(94)90352-2. PMID 8060616.
^ Calakos N, Bennett MK, Peterson KE, Scheller RH (1994). "Protein-protein interactions contributing to the specificity of intracellular vesicular trafficking". Science 263 (5150): 1146–9. doi:10.1126/science.8108733. PMID 8108733.
^ Hodel A (October 1998). "SNAP-25". Int. J. Biochem. Cell Biol. 30 (10): 1069–73. doi:10.1016/S1357-2725(98)00079-X. PMID 9785471.
^ Chapman ER (July 2002). "Synaptotagmin: a Ca(2+) sensor that triggers exocytosis?" (PDF). Nat. Rev. Mol. Cell Biol. 3 (7): 498–508. doi:10.1038/nrm855. PMID 12094216. ^{[dead link]}
^ Verderio C, Pozzi D, Pravettoni E, Inverardi F, Schenk U, Coco S et al. (February 2004). "SNAP-25 modulation of calcium dynamics underlies differences in GABAergic and glutamatergic responsiveness to depolarization". Neuron 41 (4): 599–610. doi:10.1016/S0896-6273(04)00077-7. PMID 14980208.
^ Nagy G, Milosevic I, Fasshauer D, Müller EM, de Groot BL, Lang T et al. (December 2005). "Alternative splicing of SNAP-25 regulates secretion through nonconservative substitutions in the SNARE domain". Mol. Biol. Cell 16 (12): 5675–85. doi:10.1091/mbc.E05-07-0595. PMC 1289412. PMID 16195346.
^ ^a ^b Bark, Christina (February 1995). "Differential expression of SNAP-25 protein isoforms during divergent vesicle fusion events of neural development" (PDF). Proceedings of the Nation Academies of Sciences 92 (5): 1510–1514. doi:10.1073/pnas.92.5.1510. Retrieved 18 October 2014.
^ Brophy K, Hawi Z, Kirley A, Fitzgerald M, Gill M (2002). "Synaptosomal-associated protein 25 (SNAP-25) and attention deficit hyperactivity disorder (ADHD): evidence of linkage and association in the Irish population". Mol. Psychiatry 7 (8): 913–7. doi:10.1038/sj.mp.4001092. PMID 12232787.
^ Mill J, Curran S, Kent L, Gould A, Huckett L, Richards S et al. (April 2002). "Association study of a SNAP-25 microsatellite and attention deficit hyperactivity disorder". Am. J. Med. Genet. 114 (3): 269–71. doi:10.1002/ajmg.10253. PMID 11920846.
^ Terracciano A, Sanna S, Uda M, Deiana B, Usala G, Busonero F et al. (October 2008). "Genome-wide association scan for five major dimensions of personality". Mol. Psychiatry 15 (6): 647–56. doi:10.1038/mp.2008.113. PMC 2874623. PMID 18957941.
^ Aoki KR, Guyer B (November 2001). "Botulinum toxin type A and other botulinum toxin serotypes: a comparative review of biochemical and pharmacological actions". Eur. J. Neurol. 8 Suppl 5: 21–9. doi:10.1046/j.1468-1331.2001.00035.x. PMID 11851731.
^ ^a ^b ^c Chen X, Tomchick DR, Kovrigin E, Araç D, Machius M, Südhof TC et al. (January 2002). "Three-dimensional structure of the complexin/SNARE complex". Neuron 33 (3): 397–409. doi:10.1016/s0896-6273(02)00583-4. PMID 11832227.
^ Hu K, Carroll J, Rickman C, Davletov B (November 2002). "Action of complexin on SNARE complex". J. Biol. Chem. 277 (44): 41652–6. doi:10.1074/jbc.M205044200. PMID 12200427.
^ Okamoto M, Schoch S, Südhof TC (June 1999). "EHSH1/intersectin, a protein that contains EH and SH3 domains and binds to dynamin and SNAP-25. A protein connection between exocytosis and endocytosis?". J. Biol. Chem. 274 (26): 18446–54. doi:10.1074/jbc.274.26.18446. PMID 10373452.
^ Diefenbach RJ, Diefenbach E, Douglas MW, Cunningham AL (Dec 2002). "The heavy chain of conventional kinesin interacts with the SNARE proteins SNAP25 and SNAP23". Biochemistry 41 (50): 14906–15. doi:10.1021/bi026417u. PMID 12475239.
^ ^a ^b Ilardi JM, Mochida S, Sheng ZH (February 1999). "Snapin: a SNARE-associated protein implicated in synaptic transmission". Nat. Neurosci. 2 (2): 119–24. doi:10.1038/5673. PMID 10195194.
^ ^a ^b Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H et al. (September 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. PMID 16169070.
^ Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N et al. (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
^ ^a ^b ^c ^d Hata Y, Südhof TC (June 1995). "A novel ubiquitous form of Munc-18 interacts with multiple syntaxins. Use of the yeast two-hybrid system to study interactions between proteins involved in membrane traffic". J. Biol. Chem. 270 (22): 13022–8. doi:10.1074/jbc.270.22.13022. PMID 7768895.
^ ^a ^b ^c ^d Ravichandran V, Chawla A, Roche PA (June 1996). "Identification of a novel syntaxin- and synaptobrevin/VAMP-binding protein, SNAP-23, expressed in non-neuronal tissues". J. Biol. Chem. 271 (23): 13300–3. doi:10.1074/jbc.271.23.13300. PMID 8663154.
^ ^a ^b ^c Steegmaier M, Yang B, Yoo JS, Huang B, Shen M, Yu S et al. (Dec 1998). "Three novel proteins of the syntaxin/SNAP-25 family". J. Biol. Chem. 273 (51): 34171–9. doi:10.1074/jbc.273.51.34171. PMID 9852078.
^ Dulubova I, Sugita S, Hill S, Hosaka M, Fernandez I, Südhof TC et al. (August 1999). "A conformational switch in syntaxin during exocytosis: role of munc18". EMBO J. 18 (16): 4372–82. doi:10.1093/emboj/18.16.4372. PMC 1171512. PMID 10449403.
^ McMahon HT, Missler M, Li C, Südhof TC (October 1995). "Complexins: cytosolic proteins that regulate SNAP receptor function". Cell 83 (1): 111–9. doi:10.1016/0092-8674(95)90239-2. PMID 7553862.
^ Gonelle-Gispert C, Molinete M, Halban PA, Sadoul K (September 2000). "Membrane localization and biological activity of SNAP-25 cysteine mutants in insulin-secreting cells". J. Cell. Sci. 113 ( Pt 18): 3197–205. PMID 10954418.
^ ^a ^b ^c Li Y, Chin LS, Weigel C, Li L (November 2001). "Spring, a novel RING finger protein that regulates synaptic vesicle exocytosis". J. Biol. Chem. 276 (44): 40824–33. doi:10.1074/jbc.M106141200. PMID 11524423.
^ Chapman ER, An S, Barton N, Jahn R (November 1994). "SNAP-25, a t-SNARE which binds to both syntaxin and synaptobrevin via domains that may form coiled coils". J. Biol. Chem. 269 (44): 27427–32. PMID 7961655.
^ Reed GL, Houng AK, Fitzgerald ML (April 1999). "Human platelets contain SNARE proteins and a Sec1p homologue that interacts with syntaxin 4 and is phosphorylated after thrombin activation: implications for platelet secretion". Blood 93 (8): 2617–26. PMID 10194441.
^ Gerona RR, Larsen EC, Kowalchyk JA, Martin TF (March 2000). "The C terminus of SNAP25 is essential for Ca(2+)-dependent binding of synaptotagmin to SNARE complexes". J. Biol. Chem. 275 (9): 6328–36. doi:10.1074/jbc.275.9.6328. PMID 10692432.
^ Zhang X, Kim-Miller MJ, Fukuda M, Kowalchyk JA, Martin TF (May 2002). "Ca2+-dependent synaptotagmin binding to SNAP-25 is essential for Ca2+-triggered exocytosis". Neuron 34 (4): 599–611. doi:10.1016/s0896-6273(02)00671-2. PMID 12062043.
^ Hao JC, Salem N, Peng XR, Kelly RB, Bennett MK (March 1997). "Effect of mutations in vesicle-associated membrane protein (VAMP) on the assembly of multimeric protein complexes". J. Neurosci. 17 (5): 1596–603. PMID 9030619.

External links

SNAP25 Protein at the US National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the public domain Pfam and InterPro IPR000928

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Related Pictures

Google Images Anti-SNAP25 antibody (ab31281) | Abcam Protein SNAP25 PDB 1jth.png Figure 2 - Syntaxin 1a and SNAP-25 are SNAP 25 (SP12): sc-20038. Immunoperoxidase SNAP25 - synaptosome-associated protein of synaptotagmin-synaptobrevin 2-SNAP-25 Publications of the week: SLC13A5, SNAP25

★リンクテーブル★

リンク元	「シナプトソーム関連タンパク質25」「シナプトソーム結合タンパク質25」「synaptosomal-associated protein 25」
関連記事	「SNAP」

「シナプトソーム関連タンパク質25」

Synaptosomal-associated protein, 25kDa
	PDB rendering based on 1jth.
Available structures
PDB	Ortholog search: PDBe, RCSB
List of PDB id codes
	1KIL, 1XTG, 3RK2, 3RK3, 3RL0, 3ZUR
Identifiers
Symbols	SNAP25 ; RIC-4; RIC4; SEC9; SNAP; SNAP-25; bA416N4.2; dJ1068F16.2
External IDs	OMIM: 600322 MGI: 98331 HomoloGene: 13311 GeneCards: SNAP25 Gene
Gene ontology
Molecular function	• SNARE binding; • protein binding;
Cellular component	• cytoplasm; • trans-Golgi network; • plasma membrane; • membrane; • cell junction; • growth cone; • BLOC-1 complex; • SNARE complex; • neuron projection; • synapse; • perinuclear region of cytoplasm; • synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex;
Biological process	• neurotransmitter uptake; • energy reserve metabolic process; • synaptic transmission; • neurotransmitter secretion; • glutamate secretion; • synaptic vesicle docking involved in exocytosis; • small molecule metabolic process; • regulation of insulin secretion;
	Sources: Amigo / QuickGO
RNA expression pattern
	More reference expression data
Orthologs
	v; t; e;