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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2017/03/24 14:51:47」(JST)

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English Journal

Aptamers as a replacement for antibodies in enzyme-linked immunosorbent assay.

Toh SY1, Citartan M1, Gopinath SC2, Tang TH3.
Biosensors & bioelectronics.Biosens Bioelectron.2015 Feb 15;64C:392-403. doi: 10.1016/j.bios.2014.09.026. Epub 2014 Sep 16.
The application of antibodies in enzyme-linked immunosorbent assay (ELISA) is the basis of this diagnostic technique which is designed to detect a potpourri of complex target molecules such as cell surface antigens, allergens, and food contaminants. However, development of the systematic evolution o
PMID 25278480

DNA aptamers against the Cry j 2 allergen of Japanese cedar pollen for biosensing applications.

Ogihara K, Savory N, Abe K, Yoshida W, Asahi M, Kamohara S, Ikebukuro K.
Biosensors & bioelectronics.Biosens Bioelectron.2015 Jan 15;63:159-65.
Sensing pollen allergens is required to prevent allergic disorders such as pollinosis. Aptamers, which bind to specific molecules, offer great potential as useful tools for detecting pollen allergens as measures against allergic disorders. Here, we report the identification of DNA aptamers binding t
PMID 25083924

In vitro selection of DNA-based aptamers that exhibit RNA-like conformations using a chimeric oligonucleotide library that contains two different xeno-nucleic acids.

Hagiwara K1, Fujita H, Kasahara Y, Irisawa Y, Obika S, Kuwahara M.
Molecular bioSystems.Mol Biosyst.2015 Jan 2;11(1):71-6. doi: 10.1039/c4mb00436a. Epub 2014 Oct 17.
We successfully generated chimeric DNA aptamers that contained six nucleoside analogs of 2'-O,4'-C-methylene bridged/locked nucleic acid (2',4'-BNA/LNA) in the primer region and multiple guanosine analogs of 2'-deoxy-2'-fluoro-ribonucleic acid (FNA) in the non-primer region using capillary electroph
PMID 25325213

Japanese Journal

Selection of an Aptamer against Mouse GP2 by SELEX

Hanazato Misaho,Nakato Gaku,Nishikawa Fumiko [他]
Cell Structure and Function 39(1・2), 23-29, 2014-12
NAID 40020559673

Programmed packaging for gene delivery

Hyodo M.,Sakurai Y.,Akita H.,Harashima H.
Journal of controlled release 193, 316-323, 2014-11-10
… Systematic evolution of ligands by exponential enrichment (SELEX) was applied as a new methodology for identifying a new ligand toward cell or mitochondria. …
NAID 120005525220

RNA Aptamers for Targeting Mitochondria Using a Mitochondria-Based SELEX Method

Tawaraya Yuri,Hyodo Mamoru,MstNazninAra [他]
Biological & pharmaceutical bulletin 37(8), 1411-1415, 2014-08
NAID 40020136906

Related Links

SELEX法 - 北海道大学理学部生物科学科（高分子機能学）

SELEX法 SELEX (Systematic Evolution of Ligands by EXponential enrichment) 法は，1990年代に確立された実験法で，in vitro selection法とも言われ，色素，アミノ酸，タンパク質に結合する核酸リガンド（アプタマー）の実体を明らかに ...

試験管内選択法（SELEX法） / Systematic Evolution of ...

SELEX stands for systematic evolution of ligands by exponential enrichment. This method, described primarily in 1990 [Ellington, A.D., Szostak, J.W., 1990. In vitro selection of RNA molecules that bind specific ligands. Nature 346 ...