関: Strongyloides stercoralis

English Journal

A loop-mediated isothermal amplification (LAMP) assay for Strongyloides stercoralis in stool that uses a visual detection method with SYTO-82 fluorescent dye.

Watts MR1, James G, Sultana Y, Ginn AN, Outhred AC, Kong F, Verweij JJ, Iredell JR, Chen SC, Lee R.Author information 1Centre for Infectious Diseases and Microbiology Public Health; Pathology West - Institute of Clinical Pathology and Medical Research, Westmead Hospital, New South Wales, Australia; Marie Bashir Institute for Infectious Diseases and Biosecurity and Centre for Research Excellence in Critical Infection, University of Sydney, New South Wales, Australia; Department of Zoology, University of Dhaka, Dhaka, Bangladesh; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.AbstractAn assay to detect Strongyloides stercoralis in stool specimens was developed using the loop-mediated isothermal amplification (LAMP) method. Primers were based on the 28S ribosomal subunit gene. The reaction conditions were optimized and SYTO-82 fluorescent dye was used to allow real-time and visual detection of the product. The product identity was confirmed with restriction enzyme digestion, cloning, and sequence analysis. The assay was specific when tested against DNA from bacteria, fungi and parasites, and 30 normal stool samples. Analytical sensitivity was to < 10 copies of target sequence in a plasmid and up to a 10(-2) dilution of DNA extracted from a Strongyloides ratti larva spiked into stool. Sensitivity was increased when further dilutions were made in water, indicative of reduced reaction inhibition. Twenty-seven of 28 stool samples microscopy and polymerase chain reaction positive for S. stercoralis were positive with the LAMP method. On the basis of these findings, the assay warrants further clinical validation.
The American journal of tropical medicine and hygiene.Am J Trop Med Hyg.2014 Feb;90(2):306-11. doi: 10.4269/ajtmh.13-0583. Epub 2013 Dec 9.
An assay to detect Strongyloides stercoralis in stool specimens was developed using the loop-mediated isothermal amplification (LAMP) method. Primers were based on the 28S ribosomal subunit gene. The reaction conditions were optimized and SYTO-82 fluorescent dye was used to allow real-time and visua
PMID 24323513

Images of the month: A case of strongyloidiasis presenting as a colonic mass.

Sethi S, Kheraj R, Sethi N, Wadhwa V.
The American journal of gastroenterology.Am J Gastroenterol.2014 Jan;109(1):20. doi: 10.1038/ajg.2013.448.
PMID 24402527

Strongyloides cebus (Nematoda: Strongyloididae) in Lagothrix cana (Primates: Atelidae) from the Brazilian Amazon: aspects of clinical presentation, anatomopathology, treatment, and parasitic biology.

Mati VL1, Ferreira Junior FC, Pinto HA, de Melo AL.Author information 1Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, CP 486, CEP 30123-970, Belo Horizonte, MG, Brazil.AbstractAbstract : Seven cases of parasitism by Strongyloides cebus were identified in Lagothrix cana from Brazil. Aspects of the clinical presentation, treatment, pathology, and parasitic biology of these infections are described. Moderate to severe disease was observed, requiring hospitalization of 3 primates, and diarrhea was the most common clinical sign described. One L. cana individual died, for which ulcerative enteritis was the major finding upon histopathological analysis. The use of ivermectin in these atelids was safe and effective against the parasite. Parallel attempts to experimentally infect gerbils with the parasite failed. Lagothrix cana is presented as a new host for S. cebus. The evidence that Strongyloides infections are common in nonhuman primates under free-living conditions, and even more prevalent in captive animals, likely represents a neglected problem.
The Journal of parasitology.J Parasitol.2013 Dec;99(6):1009-18. doi: 10.1645/13-288.1. Epub 2013 Aug 2.
Abstract : Seven cases of parasitism by Strongyloides cebus were identified in Lagothrix cana from Brazil. Aspects of the clinical presentation, treatment, pathology, and parasitic biology of these infections are described. Moderate to severe disease was observed, requiring hospitalization of 3 p
PMID 23909511

Japanese Journal

Rhabdias rhampholeonis n. sp. and Rhabdias mariauxi n. sp. (Nematoda, Rhabdiasoidea), first lung worms from leaf chameleons : Description, molecular evidence and notes on biology

Parasitology international : official journal of the Japanese Society of Parasitology 58(4), 375-383, 2009-12-01
NAID 10027670484

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ラ: Rhabdiasoidea
関: 糞線虫

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