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English Journal
- An improved single-round PCR leads to rapid and highly sensitive detection of Pneumocystis spp.
- Chabé M1, Khalife S2, Gantois N3, Even G4, Audebert C4.
- Medical mycology.Med Mycol.2014 Nov 1;52(8):841-6. doi: 10.1093/mmy/myu032. Epub 2014 Jun 25.
- In order to standardize a polymerase chain reaction (PCR)-based method of Pneumocystis detection, we describe the development of an improved PCR method that targets the Pneumocystis mtLSUrRNA gene. Design of a new primer pair and PCR program with suitable parameters and optimization resulted in a si
- PMID 24965947
- Comparative genomics suggests that the human pathogenic fungus Pneumocystis jirovecii acquired obligate biotrophy through gene loss.
- Cissé OH1, Pagni M2, Hauser PM3.
- Genome biology and evolution.Genome Biol Evol.2014 Jul 24;6(8):1938-48. doi: 10.1093/gbe/evu155.
- Pneumocystis jirovecii is a fungal parasite that colonizes specifically humans and turns into an opportunistic pathogen in immunodeficient individuals. The fungus is able to reproduce extracellularly in host lungs without eliciting massive cellular death. The molecular mechanisms that govern this pr
- PMID 25062922
- Molecular and nonmolecular diagnostic methods for invasive fungal infections.
- Arvanitis M1, Anagnostou T1, Fuchs BB1, Caliendo AM2, Mylonakis E3.
- Clinical microbiology reviews.Clin Microbiol Rev.2014 Jul;27(3):490-526. doi: 10.1128/CMR.00091-13.
- Invasive fungal infections constitute a serious threat to an ever-growing population of immunocompromised individuals and other individuals at risk. Traditional diagnostic methods, such as histopathology and culture, which are still considered the gold standards, have low sensitivity, which undersco
- PMID 24982319
Japanese Journal
- In Situ Detection of Aspegillus 18S Ribosomal RNA in Invasive Pulmonary Aspergillosis
- Kobayashi Makoto,Sonobe Hiroshi,Ikezoe Takayuki [他],HAKODA Eiji,OHTSUKI Yuji,TAGUCHI Hirokuni
- Internal medicine 38(7), 563-569, 1999-07
- … Specifically, ISH (LV) demonstrates hyphal elements of Aspergillus spp. … Conversely, ISH did not show positive results for any of the autopsy tissue specimens from subjects with other fungal pneumonia infections (Candida n=5, Mucor n=2, Cryptococcus n=2, and Pseudallescheria n=1), Pneumocystis carinii pneumonia (n=5), and cytomegalovirus pneumonia (n=3). … Conclusion ISH in conjunction with IHC is a useful tool for differentiating Aspergillus spp. …
- NAID 10005529472
Related Links
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- Pneumocystis is an extracellular opportunistic ascomycete fungus which rarely causes disease in dogs. This fungus causes pneumonia in immunocompromised hosts and is best known as a pathogen of human AIDS patients. The ...
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- Pneumocystis spp.
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