Percoll

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2013/11/26 12:23:15」(JST)

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Concentration of P. falciparum-infected erythrocytes by discontinuous density gradient centrifugation in Percoll.^[1]

Percoll is a tool for more efficient density separation in biochemistry that was first formulated by Pertoft et al..^[2] It is used for the isolation of cells, organelles, and/or viruses by density centrifugation. Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). Percoll is well suited for density gradient experiments because it possesses a low viscosity compared to alternatives, a low osmolarity and no toxicity towards cells and their constituents.

Percoll is a registered trademark of GE Healthcare.

Past use in artificial reproduction[edit]

Percoll was previously used in assisted reproductive technology (ART) to select sperm from semen by density gradient centrifugation, for use in techniques such as in vitro fertilization or intrauterine insemination. However, in 1996, Pharmacia sent out a letter to laboratories stating that Percoll should be used for research purposes only, not clinical. Pharmacia had not marketed it as a sperm preparation product; it had been repackaged and sold as such by third-party manufacturers. The FDA Warning Letter was due to concerns that PVP might cause damage to sperm (an unknown issue), and also that some batches of Percoll contained high levels of endotoxin (a known issue). The latter concern also applies to the use of Percoll with any other cells that might be injected back into a patient, since endotoxin can cause severe inflammation and fever. Since then it has been replaced with other colloids in the ART industry.^[3]

References[edit]

^ Rüssmann L, Jung A, Heidrich HG (1982). "The use of percoll gradients, elutriator rotor elution, and mithramycin staining for the isolation and identification of intraerythrocytic stages of plasmodium berghei". Z Parasitenkd 66 (3): 273–280. doi:10.1007/BF00925344. PMID 6177116.
^ H. Pertoft, T.C. Laurent, T. Laas and L. Kagedal. Anal. Biochem. 88 (1978), pp. 271–282 http://dx.doi.org/10.1016/0003-2697(78)90419-0
^ Mortimer, D (2000 May-Jun). "Sperm preparation methods.". Journal of Andrology 21 (3): 357–66. PMID 10819443. Retrieved 5 May 2010. Check date values in: |date= (help)

UpToDate Contents

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1. 着床前の男女生み分け技術 techniques for preimplantation sex selection

English Journal

Effect of priming in subpopulations of peripheral neutrophils from patients with chronic periodontitis.

Fredriksson MI.SourceDepartment of Periodontology, Oral Rehabilitation, Specialist Center of Public Dental Service, Västerås, Sweden.
Journal of periodontology.J Periodontol.2012 Sep;83(9):1192-9. Epub 2012 Jan 16.
Background: Patients with periodontal disease are reported to generate more reactive oxygen species (ROS) than matched controls, suggesting increased inflammatory defense activity. The purpose of this study is to determine whether there are subpopulations of peripheral neutrophils in patients with c
PMID 22248218

TM7SF1 (GPR137B): a novel lysosome integral membrane protein.

Gao J, Xia L, Lu M, Zhang B, Chen Y, Xu R, Wang L.SourceDepartment of Endocrinology and Genetic Metabolism, Yijishan Hospital of Wannan Medical College, Wuhu, China, jialing.gao@yahoo.com.
Molecular biology reports.Mol Biol Rep.2012 Sep;39(9):8883-9. Epub 2012 Jun 24.
In the previous proteomic study of human placenta, transmembrane 7 superfamily member 1 (TM7SF1) was found enriched in lysosome compartments. TM7SF1 encodes a 399-amino acid protein with a calculated molecular mass of 45 kDa. Bioinformatic analysis of its amino acid sequence showed that it is a mul
PMID 22729905

Japanese Journal

A simple method for enrichment of polychromatic erythroblasts from rat bone marrow, and their proliferation and maturation in vitro

Asano Hiroyuki,Deguchi Yoshihito,Kawamura Satoshi,Inaba Mutsumi
The Journal of Toxicological Sciences 36(4), 435-444, 2011
… To establish a method for the enrichment of polychromatic erythroblasts, bone marrow cells from 3- and 10-week-old rats were separated by discontinuous density gradient centrifugation using Percoll. …
NAID 130001034630

Transporter Associated with Antigen Processing-Like (ABCB9) Stably Expressed in Chinese Hamster Ovary-K1 Cells Is Sorted to the Microdomains of Lysosomal Membranes

Fujimoto Yasuyuki,Kamakura Aya,Motohashi Yu,Ohashi-Kobayashi Ayako,Maeda Masatomo
Biological & Pharmaceutical Bulletin 34(1), 36-40, 2011
… TAPL-GFP was co-sedimented with lysosomal marker cathepsin D on Percoll density gradient centrifugation. …
NAID 130000402259