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Immunohistochemistry and real-time PCR as diagnostic tools for detection of Borrelia burgdorferi sensu lato in ticks collected from humans.

Briciu VT1,2,3, Sebah D4, Coroiu G5, Lupşe M6, Cârstina D6, Ţăţulescu DF6, Mihalca AD7, Gherman CM7, Leucuţa D8, Meyer F4, Hizo-Teufel C9, Fingerle V9, Huber I4.
Experimental & applied acarology.Exp Appl Acarol.2016 May;69(1):49-60. doi: 10.1007/s10493-016-0012-y. Epub 2016 Jan 22.
The objective of this study was to evaluate different methods used for detection of Borrelia burgdorferi sensu lato (s.l.) in ticks: immunohistochemistry followed by focus floating microscopy (FFM) and real-time polymerase chain reaction (real-time PCR) targeting the ospA and hbb genes. Additionally
PMID 26801157

Functional Equivalence of OspA and OspB, but Not OspC, in Tick Colonization by Borrelia burgdorferi.

Tilly K1, Bestor A2, Rosa PA1.
Infection and immunity.Infect Immun.2016 Apr 22;84(5):1565-73. doi: 10.1128/IAI.00063-16. Print 2016 May.
Borrelia burgdorferi, a Lyme disease agent, makes different major outer surface lipoproteins at different stages of its mouse-tick infectious cycle. Outer surface protein A (OspA) coats the spirochetes from the time they enter ticks until they are transmitted to a mammal. OspA is required for normal
PMID 26953324

Embers ME1, Hasenkampf NR2, Barnes MB3, Didier ES3, Philipp MT2, Tardo AC2.
Clinical and vaccine immunology : CVI.Clin Vaccine Immunol.2016 Apr 4;23(4):294-303. doi: 10.1128/CVI.00685-15. Print 2016 Apr.
The systematically difficult task of diagnosing Lyme disease can be simplified by sensitive and specific laboratory tests. The currently recommended two-tier test for serology is highly specific but falls short in sensitivity, especially in the early acute phase. We previously examined serially coll
PMID 26843487