English Journal

Human Colorectal Cancer Stage-dependent Global DNA Hypomethylation of Cancer-associated Fibroblasts.

Ling E, Ringel A, Sigal-Batikoff I, Abu-Freha N, Vaknine H, Friah W, Reshef A, Pinsk I, Fich A, Lamprecht S.
Anticancer research. 2016 09;36(9)4503-7.
Cancer-associated fibroblasts (CAFs) play an important role in tumor development and progression. The prevailing consensus favors the view that a specific epigenetic signature underpins the stable CAF phenotype. The aim of the present study was to assess global DNA methylation in CAFs during the ade
PMID 27630288

Is the contribution of cis and trans protonated 5-methylcytosine-SO3(-) isomers equal in the conversion to thymine-SO3(-) under bisulfite conditions? A theoretical perspective.

Jin L, Wang L, Zhang C, Wang W, Min S, Hu D.
Physical chemistry chemical physics : PCCP. 2014 Aug;16(30)16264-77.
Cytosine (Cyt) can be converted to 5-methylcytosine (5-MeCyt) in CpG sequences of DNA. Conventional bisulfite sequencing can discriminate Cyt from 5-MeCyt, however inappropriate conversion of 5-MeCyt to thymine and a failure to convert Cyt to uracil always occur when Cyt and 5-MeCyt are treated with
PMID 24974803

A new insight into the 5-carboxycytosine and 5-formylcytosine under typical bisulfite conditions: a deamination mechanism study.

Jin L, Wang W, Hu D, Lü J.
Physical chemistry chemical physics : PCCP. 2014 Feb;16(8)3573-85.
5-Methylcytosine (5-MeCyt) can be converted to 5-hydroxymethylcytosine (5-hmCyt) in mammalian DNA by the ten-eleven translocation enzymes. The conventional bisulfite sequencing cannot discriminate 5-hmCyt from 5-MeCyt, whereas the oxidation products of 5-hmCyt, 5-carboxycytosine (5-caCyt) and 5-form
PMID 24413472

Japanese Journal

山崎和幸,深沢芳隆,中村文彦,須山享三
日本畜産学会報 64(5), 512-517, 1993
… -MeCyt)を,牛および豚の肺および胸腺の加水分解物から分離調製する方法について検討した.これらの塩基は活性炭カラムおよび大口径シリカゲルカラムにより分離した.それぞれCytおよび5-MeCytは,カウンターイオンとしてSDSを用いた逆相系分取HPLCにより精製し,結晶として得た.一方で,これらの塩基の逆相HPLCによる分析法を開発し,定量を行なった.その結果,乾物1g当りのCytおよび5-MeCyt …
NAID 130000750304