Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references
MOPS (3-(N-morpholino)propanesulfonic acid) is a buffer introduced by Good et al. in the 1960s. It is a structural analog to MES.[1] Its chemical structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.
Contents
1Applications
2See also
3References
4External links
Applications
MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis.[2] Usage above 20 mM in mammalian cell culture work is not recommended.[3]
See also
CAPS
HEPPS
Tris
Common buffer compounds used in biology
References
^Good, Norman E.; Winget, G. Douglas; Winter, Wilhelmina; Connolly, Thomas N.; Izawa, Seikichi; Singh, Raizada M. M. (1966). "Hydrogen Ion Buffers for Biological Research". Biochemistry. 5 (2): 467–77. doi:10.1021/bi00866a011. PMID 5942950.
^Thomas, J; Hodes, ME (1981). "A new discontinuous buffer system for the electrophoresis of cationic proteins at near-neutral pH". Analytical Biochemistry. 118 (1): 194–6. doi:10.1016/0003-2697(81)90178-0. PMID 6278979.
^Eagle, H. (1971). "Buffer Combinations for Mammalian Cell Culture". Science. 174 (4008): 500–3. doi:10.1126/science.174.4008.500. PMID 5110427.
External links
Sigma Aldrich Buffer Calculator - Useful tool to calculate weight, volume, or concentration from molecular weight.
Recipe for MOPS buffer on OpenWetWare
English Journal
A new type of polyhedron-based metal-organic frameworks with interpenetrating cationic and anionic nets demonstrating ion exchange, adsorption and luminescent properties.
Zhang ZJ, Shi W, Niu Z, Li HH, Zhao B, Cheng P, Liao DZ, Yan SP.SourceDepartment of Chemistry, Nankai University, Tianjin 300071, P. R. China. shiwei@nankai.edu.cn pcheng@nankai.edu.cn.
Chemical communications (Cambridge, England).2011 Jun 14;47(22):6425-7. Epub 2011 May 9.
An interesting new MOF, built with interpenetrating cationic (MOF-A(+)) and anionic (MOF-B(-)) nets that do not require counter ions to balance charge, together with an architectural strategy focused on the use of MOPs as nodes and MOCs as spacers for the generation of 3D frameworks, is reported.
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