HpaII

• restriction enzyme HpaII

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2017/03/31 21:03:17」(JST)

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HpaII (IntEnz: EC 3.1.21.4) is a restriction enzyme obtained from the microorganism called Haemophilus parainfluenzae. It is a DNA restriction enzyme, therefore it has the ability to cut the DNA from certain region as demonstrated below. It has the ability to produce cohesive ends, which are rather useful in constructing plasmids.

Recognition sequence	Cut results
5' CCGG 3' GGCC	5' C CGG 3' GGC C

HpaII will not cut sites that have been methylated by SssI methyltransferase or HpaII methyltransferase. When the sites have been methylated by MspI methyltransferase, the enzyme will cut 300 times slower than unmethylated DNA and 50 times slower if the DNA is hemi-methylated. This feature is exploited for determination of the clonal origin of a mammalian female tumor through HUMARA assay.

References

English Journal

• A dual amplification fluorescent strategy for sensitive detection of DNA methyltransferase activity based on strand displacement amplification and DNAzyme amplification.

• Cui W1, Wang L2, Jiang W3.
• Biosensors & bioelectronics.Biosens Bioelectron.2016 Mar 15;77:650-5. doi: 10.1016/j.bios.2015.10.040. Epub 2015 Oct 22.
• DNA methyltransferase (MTase) plays a critical role in many biological processes and has been regarded as a predictive cancer biomarker and a therapeutic target in cancer treatment. Sensitive detection of DNA MTase activity is essential for early cancer diagnosis and therapeutics. Here, we developed
• PMID 26492469

• Clonality assessment of adenomatoid tumor supports its neoplastic nature.

• Wang W1, Zhu H2, Wang J1, Wang S1, Wang D3, Zhao J2, Zhu H4.
• Human pathology.Hum Pathol.2016 Feb;48:88-94. doi: 10.1016/j.humpath.2015.09.032. Epub 2015 Oct 23.
• Adenomatoid tumor is a relatively rare disease that predominantly involves male and female internal genital tracts. Although its clinical and pathologic features are well characterized, there is still controversy regarding its nature as a true neoplasm or a variant of mesothelial hyperplasia of a re
• PMID 26772404

• Haemophilia A and cardiovascular morbidity in a female SHAM syndrome carrier due to skewed X chromosome inactivation.

• Janczar S1, Kosinska J2, Ploski R2, Pastorczak A1, Wegner O1, Zalewska-Szewczyk B1, Paige AJ3, Borowiec M1, Mlynarski W4.
• European journal of medical genetics.Eur J Med Genet.2016 Jan;59(1):43-7. doi: 10.1016/j.ejmg.2015.12.004. Epub 2015 Dec 10.
• We have recently described a severe haemophilia A and moyamoya (SHAM) syndrome caused by Xq28 deletions encompassing F8 and the BRCC3 familial moyamoya gene. The phenotype includes haemophilia A, moyamoya angiopathy, dysmorphia and hypertension. The genetic analysis of the family of our SHAM patient
• PMID 26691666

Japanese Journal

• Genome-wide profiling of promoter methylation in human

• Oncogene 25(21), 3059-3064, 2006-05-18
• NAID 120000908395

• DNA Methylation Polymorphisms in Rice and Wild Rice Strains : Detection of Epigenetic Markers

• Breeding science 55(1), 57-63, 2005-03
• NAID 110001818712

• HpaII polymorphism in the atrial natriuretic peptide gene and hypertension

• Am J Hypertens 12, 524-527, 1999
• NAID 30008925346

Related Links

• HpaII (R0171) - New England Biolabs Japan

ニュー・イングランド・バイオラボ －NEBは制限酵素、エンドヌクレアーゼ、リコンビナント酵素、PCR用試薬、発現システム、マーカー、コンピテントセル、RNA研究用試薬、ポリメラーゼ、修飾酵素、核酸、細胞解析などの試薬を提供 ...

• HpaII | NEB

HpaII CutSmart® Buffer Legal and Disclaimers Legal and Disclaimers This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). While NEB develops and ...

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拡張検索	「制限酵素HpaII」「deoxyribonuclease HpaII」「restriction enzyme HpaII」

「制限酵素HpaII」

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restriction enzyme HpaII、deoxyribonuclease HpaII

「deoxyribonuclease HpaII」

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制限酵素HpaII

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restriction enzyme HpaII

「restriction enzyme HpaII」

　　[★]

制限酵素HpaII

関

deoxyribonuclease HpaII