English Journal

Optochemical Control of Deoxyoligonucleotide Function via a Nucleobase-Caging Approach.

Liu Q, Deiters A.Author information Department of Chemistry, North Carolina State University , Raleigh, North Carolina 27695, United States.AbstractSynthetic oligonucleotides have been extensively applied tocontrol a wide range of biological processes such as gene expression, gene repair, DNA replication, and protein activity. Based on well-established sequence design rules that typically rely on Watson-Crick base pairing interactions researchers can readily program the function of these oligonucleotides. Therefore oligonucleotides provide a flexible platform for targeting a wide range of biological molecules, including DNA, RNA, and proteins. In addition, oligonucleotides are commonly used research tools in cell biology and developmental biology. However, a lack of conditional control methods has hampered the precise spatial and temporal regulation of oligonucleotide activity, which limits the application of these reagents to investigate complex biological questions. Nature controls biological function with a high level of spatial and temporal resolution and in order to elucidate the molecular mechanisms of biological processes, researchers need tools that allow for the perturbation of these processes with Nature's precision. Light represents an excellent external regulatory element since irradiation can be easily controlled spatially and temporally. Thus, researchers have developed several different methods to conditionally control oligonucleotide activity with light. One of the most versatile strategies is optochemical regulation through the installation and removal of photolabile caging groups on oligonucleotides. To produce switches that can control nucleic acid function with light, chemists introduce caging groups into the oligomer backbone or on specific nucleobases to block oligonucleotide function until the caging groups are removed by light exposure. In this Account, we focus on the application of caged nucleobases to the photoregulation of DNA function. Using this approach, we have both activated and deactivated gene expression optochemically at the transcriptional and translational level with spatial and temporal control. Specifically, we have used caged triplex-forming oligomers and DNA decoys to regulate transcription, and we have regulated translation with light-activated antisense agents. Moreover, we also discuss strategies that can trigger DNA enzymatic activity, DNA amplification, and DNA mutagenesis by light illumination. More recently, we have developed light-activated DNA logic operations, an advance that may lay the foundation for the optochemical control of complex DNA calculations.
Accounts of chemical research.Acc Chem Res.2014 Jan 21;47(1):45-55. doi: 10.1021/ar400036a. Epub 2013 Aug 28.
Synthetic oligonucleotides have been extensively applied tocontrol a wide range of biological processes such as gene expression, gene repair, DNA replication, and protein activity. Based on well-established sequence design rules that typically rely on Watson-Crick base pairing interactions researche
PMID 23981235

Identification of inhibitors of microRNA function from small molecule screens.

Connelly CM, Deiters A.Author information Department of Chemistry, North Carolina State University, Raleigh, NC, USA.AbstractAberrant expression of microRNAs (miRNAs) has been linked to many human diseases including cancer, immune disorders, heart disease, and viral infections. Thus, small molecule inhibitors of miRNAs have potential as new therapeutic agents, as probes for the elucidation of detailed mechanisms of miRNA function, and as tools for the discovery of new targets for the treatment of human diseases. In order to identify small molecule inhibitors of specific miRNAs, functional assays have been developed and applied to the screening of small molecule libraries. Here, we report the application of a luciferase-based reporter assay of miRNA miR-122 function to the discovery of small molecule miR-122 inhibitors.
Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2014;1095:147-56. doi: 10.1007/978-1-62703-703-7_12.
Aberrant expression of microRNAs (miRNAs) has been linked to many human diseases including cancer, immune disorders, heart disease, and viral infections. Thus, small molecule inhibitors of miRNAs have potential as new therapeutic agents, as probes for the elucidation of detailed mechanisms of miRNA
PMID 24166310

Cellular microRNA sensors based on luciferase reporters.

Connelly CM, Deiters A.Author information Department of Chemistry, North Carolina State University, Raleigh, NC, USA.AbstractRecently, microRNAs (miRNAs) have been linked to a variety of human diseases including cancer and viral infections. Small molecule modifiers of miRNAs could represent new therapeutic agents and be used as tools for elucidating the biological roles of miRNAs. In order to identify small molecule modifiers of miRNAs, functional assays for specific miRNAs must be developed and optimized. Here, we report the construction of a luciferase reporter assay for miRNA miR-122 function and the development of a stable Huh7 cell line that can be used for high-throughput screening of small molecule miR-122 inhibitors. The steps described here can be applied not only to Huh7 cells and miR-122 but also to virtually any cell line and miRNA combination.
Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2014;1095:135-46. doi: 10.1007/978-1-62703-703-7_11.
Recently, microRNAs (miRNAs) have been linked to a variety of human diseases including cancer and viral infections. Small molecule modifiers of miRNAs could represent new therapeutic agents and be used as tools for elucidating the biological roles of miRNAs. In order to identify small molecule modif
PMID 24166309

Japanese Journal

モルモット蝸牛ダイテルス細胞のK電流の電気生理学的特性

縄手彩子,君付隆,玉江昭裕,松本希,柴田修明,大橋充,小宗静男
Otology Japan 20(1), 1-6, 2010-02-25
内耳蝸牛のダイテルス細胞は、Kイオンリサイクルシステムの一部でありgap junctionを通じてＫイオンの流入、流出が行われる。今回、Kイオン動態の一部として重要と考えられるK チャネル特性を明らかにするために、ダイテルス細胞のK電流について研究を行った。ダイテルス細胞は酵素（trypsin）と機械的操作により単離し、形態学的特徴により同定可能であった。K電流はパッチクランプ法のconventi …
NAID 10026280628

A Simple Mixing Rule for the Deiters Equation of State : Prediction of Interaction Second Virial Coefficients and PVTx Properties of Binary Mixtures

Jalili Amir Hossein,Khodagholi Mohammad Ali,Nonahal Behrouz
Journal of chemical engineering of Japan 40(3), 203-212, 2007-03-01
… are estimated by means of the Deiters equation of state (EoS). … The binary interaction parameters for these combining rules are calculated by fitting the expression for the interaction second virial coefficient derived from the Deiters EoS to the experimental cross virial coefficient data. … values from the optimized binary interaction parameters and Deiters EoS are in good agreement with experimental data. …
NAID 10018902622

Firing Patterns of Rat Vestibulospinal Neurons during Quadrupedal Standing on a Pitching Platform

Fujiwara S.,Saito T.,Tian F. [他],YAMAGUCHI T.
The journal of physiological sciences : JPS 56(5), 389-392, 2006-10-01
NAID 10019380255

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