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the 3rd letter of the Roman alphabet (同)c
(music) the keynote of the scale of C major
a general-purpose programing language closely associated with the UNIX operating system
the 5th letter of the Roman alphabet (同)e
the base of the natural system of logarithms; approximately equal to 2.718282...

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2014/11/26 22:33:36」(JST)

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CFU-E is a colony forming unit.

It arises from CFU-GEMM

mouse CFU-e colonies stained on Day 3 with Diaminobenzidine for hemoglobin

Understanding the murine CFU-e assay (analogous to human assay):

CFU-e is a stage of erythroid development between the BFU-e stage and the pro-erythroblast stage. CFU-e colony assay is designed to detect how many colony-forming-units of erythroid lineage there are in a hematopoietic tissue (bone marrow, spleen, or fetal liver), which may be reflective of the organism’s demand for oxygen delivery to the tissues or a hematopoietic disorder.

Early erythroid progenitors are found at a quite low frequency relative to later stages of erythroid differentiation, such as the pro-erythroblast and the basophilic erythroblast stages which can be detected by flowcytometry directly ex-vivo (Socolovsky et al. 2001 PMID 11719363). Furthermore, unlike for the pro-erythroblast and later stages of erythroid development, no truly reliable and unique positive flow-cytometric markers exist, though it is possible to use negative exclusion markers to deplete a cell population of other precursors and differentiated cells by cell sorting, thus greatly enriching it for the CFU-e activity (Prospective isolation and global gene expression analysis of the erythrocyte colony-forming unit, Terszowski G. et al. Blood 2005). CFU-e cells express Epo receptor, c-Kit (Stem cell factor receptor), transferrin receptor (CD71+), and are Ter119(glycophorin-A associated antigen)-negative. For the above reasons, the CFU-e assay, as inefficient and variable as it can often be, is still in use today.

Cells at the CFU-e stage express some erythropoietin receptor (EpoR), and thus can be induced to terminally differentiate in vitro in 2–3 days in the presence of only erythropoietin (Epo) (together with the basic contents of culture media: FBS, BSA in IMDM). Methylcellulose is a semisolid media additive that allows an investigator to stain (with diaminobenzidine reagent for hemoglobin) and then count individual colonies, each arising from a single plated progenitor that is at the CFU-e stage. By day 2 from the time of plating, each CFU-e colony will contain between 8 (minimum) and 64 hemoglobinized cells most of which are in their end-stage of erythroid differentiation. It is possible to see a small spectrum of hemoglobinization level and possibly cell size, indicating that some cells in the colony have achieved the end-stage faster than others.

Cell number in a colony is important because pro-erythroblast stage is also Epo-responsive (expresses Epo receptor), but the proliferative capacity of these cells is not as high, thus yielding a colony with fewer than 8 cells. Likewise, an earlier stage of erythroid differentiation may also yield colonies in Epo-only medium, but these colonies would likely be smaller and/or not hemoglobinized, since the stages before the CFU-e stage (MEP and BFU-e) require other factors (IL-3 etc.) and more time for growth that will also delay the terminal differentiation and hemoglobinization.

(via BFU-E,^[1] which stands for "erythroid burst-forming units"^[2]) and gives rise to proerythroblasts.

References

^ Wu H, Liu X, Jaenisch R, Lodish HF (October 1995). "Generation of committed erythroid BFU-E and CFU-E progenitors does not require erythropoietin or the erythropoietin receptor". Cell 83 (1): 59–67. doi:10.1016/0092-8674(95)90234-1. PMID 7553874.
^ Marley SB, Lewis JL, Goldman JM, Gordon MY (June 1996). "Abnormal kinetics of colony formation by erythroid burst-forming units (BFU-E) in chronic myeloid leukaemia". Br. J. Haematol. 93 (4): 878–83. doi:10.1046/j.1365-2141.1996.d01-1738.x. PMID 8703820.

External links

CFU-E at the US National Library of Medicine Medical Subject Headings (MeSH)

UpToDate Contents

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1. 赤血球産生調節 regulation of erythropoiesis
2. 貧血を有する成人患者に対するアプローチ approach to the adult patient with anemia
3. 造血および幹細胞の機能の概要 overview of hematopoiesis and stem cell function
4. Chapter 6J：エリスロポエチン chapter 6j erythropoietin
5. 赤血球産生低下による貧血 anemias due to decreased red cell production

English Journal

Effects by anthrax toxins on hematopoiesis: A key role for cytokines as mediators.

Rameshwar P, Wong EW, Connell ND.SourceDepartment of Medicine, UMDNJ-New Jersey Medical School, Newark, NJ 07103, United States.
Cytokine.Cytokine.2011 Nov 12. [Epub ahead of print]
An understanding of anthrax toxins on the emerging immune system and blood production are significant to medicine. This study examined the effects of anthrax toxin on hematopoiesis and determined roles for cytokines. Anthrax holotoxin toxin is three components: protective antigen (PA) binds to the t
PMID 22082805

Enucleation of human erythroblasts involves nonmuscle myosin IIB.

Ubukawa K, Guo YM, Takahashi M, Hirokawa M, Michishita Y, Nara M, Tagawa H, Takahashi N, Komatsuda A, Nunomura W, Takakuwa Y, Sawada K.SourceDepartment of Hematology, Nephrology, and Rheumatology, Akita University School of Medicine, Akita, Japan;
Blood.Blood.2011 Nov 2. [Epub ahead of print]
Mammalian erythroblasts undergo enucleation, a process thought to be similar to cytokinesis. Although an assemblage of actin, nonmuscle myosin II, and several other proteins is crucial for proper cytokinesis, the role of nonmuscle myosin II in enucleation remains unclear. In this study, we investiga
PMID 22049517

Japanese Journal

CXCR4 Gene Transfer Enhances The Distribution of Dermal Multipotent Stem Cells to Bone Marrow in Sublethally Irradiated Rats

ZONG Zhao-Wen,XIANG Qiang,CHENG Tian-Min,DONG Shi-Wu,SU Yong-Ping,LI Nan,RAN Xin-Ze,SHI Chun-Men,AI Guo-Ping
Journal of radiation research 50(3), 193-201, 2009-05-16
… 0.05), and the amount of CFU-F, CFU-E and CFU-GM were greater than that in group B and group C from day 14 after injury (P < …
NAID 10025911648

Effects of Four Si-Wu-Tang's Constituents and Their Combination on Irradiated Mice(Pharmacology)

LIANG Qian-De,GAO Yue,TAN Hong-Ling,GUO Ping,LI Ying-Fei,ZHOU Zhe,TAN Wei,MA Zeng-Chun,MA Bai-Ping,WANG Sheng-Qi
Biological & pharmaceutical bulletin 29(7), 1378-1382, 2006-07-01
… Similarly to SWT, oral administration of Fru, Pae, FA, TP and their combination, to some extent, all showed effects of increasing the number of peripheral leukocyte and increasing four types of progenitor cells in bone marrow, including colony-forming unit-granulocyte-macrophage (CFU-GM), colony-forming unit-mature erythroid (CFU-E), colony-forming unit-immature erythroid (BFU-E) and colony-forming unit-multipotential (CFU-mix). …
NAID 110005602305

Related Pictures

★リンクテーブル★

リンク元	「エリスロポエチン」「赤血球」「赤芽球コロニー形成単位」「赤芽球バースト形成細胞」「前赤芽球」
関連記事	「CF」「C」「CFU」「Es」「E」

「エリスロポエチン」

　　[★]

英: erythropoietin EPO EP Ep
同: 赤血球生成促進因子 erythropoiesis-stimulating factor、エリスロポイエチン、ヒトエリスロポエチン human erythropoietin
商: epoetin alfa, Epogen, Procrit

種類

分類

性状

糖タンパク質
34kDa

産生組織

腎臓の傍糸球体細胞

the interstitium of the renal parenchyma, outside the tubular basement membrane , mostly in the inner cortex and outer medulla. (WINTEROBE'S CLINICAL HEMATOLOGY. 209)

標的組織

分化赤血球系前駆細胞(CFU-E)

受容体

作用

後期赤芽球系前駆細胞の赤芽球分化
CFU-Eを前赤芽球に分化誘導する。

分泌の調整

EPO分泌↑

血液中の酸素分圧低下

失血、気圧減少、肺疾患、心疾患

エリスロポエチン産生腫瘍

小脳血管芽細胞種、肝癌、腎癌

EPO分泌↓

大量輸血

低酸素状態に対するエリスロポエチンによる代償機構の発現には数日かかる。2,3-DPGによる代償機構は2日。(SP.489,654)

分子機構

低酸素刺激で発現誘導される転写因子HIF(ヘテロダイマー：HIF-1α、HIF-1β)がエリスロポエチンの発現に関与

生合成

基準範囲

7.4～29.8mIU/mL

判断

高値

貧血、骨髄異形成症候群、再生不良性貧血、鉄欠乏性貧血、偽性多血症、二次性赤血球増加症、再生不良性貧血、骨髄異形成症候群、鉄欠乏性貧血、慢性疾患に伴う貧血の一部、偽性多血症、二次性赤血球増加症(組織の低酸素状態、EPO産生増加状態)

低値

貧血、多発性骨髄腫、慢性腎不全、膠原病、真性多血症、腎性貧血、慢性炎症性疾患、慢性感染症、慢性腎不全に伴う腎性貧血、真性多血症、多発性骨髄腫、慢性炎症性疾患(慢性感染症、膠原病など)に伴う貧血の一部

臨床関連

腎性貧血

腎不全で分泌↓

「赤血球」

　　[★]

英: erythrocyte (Z), red blood cell (Z), RBC
関: 血液、貧血、血液像

赤血球の形状

直径：7.62±0.61μm　→　7-8μm
厚さ：1.0-2.0μm
容積：90μm³
表面積：130μm²
比重：1.097

分化

造血幹細胞→骨髄系前駆細胞(CFU-GEMM)→未分化赤血球系前駆細胞(BFU-E)→分化赤血球系前駆細胞(CFU-E)→前赤芽球→塩基性赤芽球→多染性赤芽球→正染性赤芽球→網状赤血球→赤血球

基準値

2007前期解剖学授業プリント
♂：420万 - 570万 (/μl)　　495万±75万 x 10^6 (/μl)
♀：380万 - 550万 (/μl)　　465万±85万 x 10^6 (/μl)
-2007前期生理学授業プリント
♂：450万 - 610万 /μl
♀：380万 - 530万 /μl
-PT.233
♂：500万 /mm3
♀：450万 /mm3
-異常値の出るメカニズム第5版 p.79
男：400万-700万/ul
女：350万-600万/ul
-HIM
男：430–560 x 10^4/μl
女：400–520 x 10^4/μl

寿命

100-120日　→　4ヶ月

赤血球の増加

テストステロン、低酸素、コルチゾール、エリスロポエチン

「赤芽球コロニー形成単位」

　　[★]

英: colony-forming unit-erythroid colony-forming units-erythroid colony forming unit erythroid CFU-E, erythroid colony forming unit
同: 赤芽球コロニー形成細胞 erythroid colony-forming cell、分化赤血球系前駆細胞 (血液細胞アトラス)
関: 赤血球

BFU-E<-CFU-E->塩基性赤芽球

造血系前細胞
前赤芽球

分化

造血幹細胞		造血幹細胞
多能性前駆細胞		骨髄系前駆細胞 CFU-GEMM
造血前駆細胞		未分化赤血球前駆細胞 BFU-E
		分化赤血球前駆細胞 CFU-E
分化		前赤芽球
		好塩基性赤芽球
		多染性赤芽球
		正染性赤芽球
	(血液へ)	網赤血球
		赤血球

-CFU-E

「赤芽球バースト形成細胞」

　　[★]

英: burst-forming unit-erythroid burst forming unit erythroid, BFU-E
同: 前期赤芽球系前駆細胞、赤芽球バースト形成単位、分化赤血球系前駆細胞 (血液細胞アトラス)
関: 赤芽球

分化

造血幹細胞		造血幹細胞
多能性前駆細胞		骨髄系前駆細胞 CFU-GEMM
造血前駆細胞		未分化赤血球前駆細胞 BFU-E
		分化赤血球前駆細胞 CFU-E
分化		前赤芽球
		好塩基性赤芽球
		多染性赤芽球
		正染性赤芽球
	(血液へ)	網赤血球
		赤血球

「前赤芽球」

　　[★]

英: proerythroblast
関: 赤血球、血球

CFU-E<-前赤芽球->好塩基性赤芽球

分化

造血幹細胞→骨髄系幹細胞→前赤芽球→好塩基性赤芽球→多染性赤芽球→正染性赤芽球→網状赤血球→赤血球

特徴

核小体（＋）

「CF」

　　[★]

冠状動脈血流冠動脈血流 coronary flow
補体結合反応試験 complement fixation test
嚢胞性線維症 cystic fibrosis
ロイコボリン leucovorin calcium
登上線維 climbing fiber climbing fibre
楔状束 cuneate fasciculus
冠血流 coronary flow
大腸ファイバースコープ colon fiberscope、大腸内視鏡、下部消化管内視鏡

「C」

　　[★]

炭素 carbon
シトシン cytosine
システイン cysteine
シチジン
セルシウス度摂氏 degrees Celsius degree C
産婦人科領域で「子宮外頚子宮外頚部 exocervix」を表現するための略語

「CFU」

　　[★] コロニー形成単位

同: colony-forming units

「Es」

　　[★] アインスタイニウム

関: einsteinium

「E」

　　[★]

[pmid7553874-1] Wu H, Liu X, Jaenisch R, Lodish HF (October 1995). "Generation of committed erythroid BFU-E and CFU-E progenitors does not require erythropoietin or the erythropoietin receptor". Cell 83 (1): 59–67. doi:10.1016/0092-8674(95)90234-1. PMID 7553874.

[pmid8703820-2] Marley SB, Lewis JL, Goldman JM, Gordon MY (June 1996). "Abnormal kinetics of colony formation by erythroid burst-forming units (BFU-E) in chronic myeloid leukaemia". Br. J. Haematol. 93 (4): 878–83. doi:10.1046/j.1365-2141.1996.d01-1738.x. PMID 8703820.

匿名

検索

案内

CFU-E